Hydrophobin signal sequence mediates efficient secretion of recombinant proteins in Pichia pastoris

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Applied Microbiology and Biotechnology

• Publication date

  2011-04-12

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1007/s00253-011-3246-yPMID 21484207
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• RT-PCR Protocols - Methods in Molecular Biology

  2011cited by this paper
• Expression and characterization of a Grifola frondosa hydrophobin in Pichia pastoris.

  2010influential reference
• Structural analysis of hydrophobins.

  2008cited by this paper
• Pichia Protocols

  2007cited by this paper
• Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria.

  2006cited by this paper
• Comparison of different signal peptides for secretion of heterologous proteins in fission yeast.

  2005cited by this paper
• Viral Preprotoxin Signal Sequence Allows Efficient Secretion of Green Fluorescent Protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

  2004cited by this paper
• Improved prediction of signal peptides: SignalP 3.0.

  2004influential reference
• Hydrophobins: multipurpose proteins.

  2001cited by this paper
• Heterologous protein expression in the methylotrophic yeast Pichia pastoris.

  2000influential reference
• Recombinant protein expression in Pichia pastoris

  2000cited by this paper
• A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris

  1998cited by this paper
• The green fluorescent protein.

  1998cited by this paper
• Overexpression of Human Procarboxypeptidase A2 in Pichia pastoris and Detailed Characterization of Its Activation Pathway*

  1998cited by this paper
• Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei--cloning and characterization of the hfb2 gene.

  1997influential reference
• Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.

  1997cited by this paper
• Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris.

  1997cited by this paper
• Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.

  1997cited by this paper
• SHORT COMMUNICATION Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites

  1997influential reference
• Genetic and biochemical characterization of the Trichoderma reesei hydrophobin HFBI.

  1996cited by this paper
• GREEN FLUORESCENT PROTEIN

  1995cited by this paper
• Foreign gene expression in yeast: a review

  1992cited by this paper
• Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

  1982cited by this paper
• Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.

  1982cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• AlphaFold modeling uncovers global structural features of class I and class II fungal hydrophobins

  2025cites this paper
• Protein secretion and associated stress in industrially employed filamentous fungi

  2024cites this paper
• Alternative secretory signal sequences for recombinant protein production in Pichia pastoris.

  2023cites this paper
• Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33.

  2023cites this paper
• Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.

  2022cites this paper
• Potential of the Signal Peptide Derived from the PAS_chr3_0030 Gene Product for Secretory Expression of Valuable Enzymes in Pichia pastoris

  2022cites this paper
• At least three families of hyphosphere small secreted cysteine‐rich proteins can optimize surface properties to a moderately hydrophilic state suitable for fungal attachment

  2021cites this paper
• Soluble Expression and Efficient Purification of Recombinant Class I Hydrophobin DewA

  2021cites this paper
• Extracellular vesicles carry cellulases in the industrial fungus Trichoderma reesei

  2019cites this paper
• Established and Upcoming Yeast Expression Systems.

  2019cites this paper
• Comparison of Yeasts as Hosts for Recombinant Protein Production

  2018cites this paper
• High-level expression and purification of a molluskan endoglucanase from Ampullaria crossean in Pichia pastoris.

  2017cites this paper
• Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

  2017cites this paper
• Yeast Expression Systems: Current Status and Future Prospects

  2017cites this paper
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  2017cites this paper
• Fungal Biotechnology for Industrial Enzyme Production: Focus on (Hemi)cellulase Production Strategies, Advances and Challenges

  2016cites this paper
• Differential secretion pathways of proteins fused to the Escherichia coli maltose binding protein (MBP) in Pichia pastoris.

  2016cites this paper
• Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

  2016cites this paper
• Development of an expression system for heterologous protein expression in fungus

  2016cites this paper
• Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

  2016cites this paper
• Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

  2015cites this paper
• Improved extracellular endo-1,4-β-mannosidase activity of recombinant Pichia pastoris by optimizing signal peptide

  2015cites this paper
• Signal peptides from filamentous fungi efficiently mediate the secretion of recombinant proteins in Kluyveromyces lactis

  2015cites this paper
• Improved extracellular endo-1,4-β-mannosidase activity of recombinant Pichia pastoris by optimizing signal peptide

  2015cites this paper
• Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization.

  2015cites this paper
• Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

  2015cites this paper
• In-silico determination of Pichia pastoris signal peptides for extracellular recombinant protein production.

  2015cites this paper
• Promoter and signal sequence from filamentous fungus can drive recombinant protein production in the yeast Kluyveromyces lactis.

  2014cites this paper
• Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

  2014cites this paper
• Yeast Metabolic Engineering

  2014cites this paper
• Efficient secretion of inulin fructotransferase in Pichia pastoris using the formaldehyde dehydrogenase 1 promoter

  2014cites this paper
• Pichia pastoris: a workhorse for recombinant protein production

  2014cites this paper
• Strains and molecular tools for recombinant protein production in Pichia pastoris.

  2014cites this paper
• The Secretory Pathway in the Filamentous Fungus Trichoderma

  2014cites this paper
• Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins.

  2013cites this paper
• Recombinant tagging system using ribosomal frameshifting to monitor protein expression

  2013cites this paper
• Pichia pastoris: protein production host and model organism for biomedical research.

  2013cites this paper
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  2012cites this paper
• Factors affecting the specific productivity of Pichia pastoris

  2012cites this paper
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  2012cites this paper
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  2012cites this paper