Escherichia coli CorA Periplasmic Domain Functions as a Homotetramer to Bind Substrate*

CorA is a primary Mg2+ transporter in bacteria, which also mediates influx of Ni2+ and Co2+. Topological studies suggested that it could be divided into a large soluble periplasmic domain (PPD) and three membrane-spanning α-helixes. In the present study, glutathione S-transferase (GST) fusion Escherichia coli CorA PPD was purified by GST affinity chromatography, and PPD was obtained by on-column thrombin digestion. Size-exclusion chromatography indicated that purified PPD exists as a homotetramer. Single particle electron microscopy analysis of PPD and two-dimensional crystals of GST-PPD indicated that E. coli CorA PPD is a pyramid-like homotetramer with a central cavity. Comparison of the CD spectra of full-length CorA and PPD also suggested that PPD has similar secondary structure to the full-length CorA. Dissociation constants for CorA and PPD with their substrates, determined by dose-dependent fluorescence quench of ligands, suggested that purified PPD retains its substrate binding ability as native CorA. The CorA PPD structure described here may provide structural information for the E. coli CorA functional oligomeric state.

• Publication year

  2006

• Venue

  Journal of Biological Chemistry

• Publication date

  2006-09-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.M602342200PMID 16835234
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Crystallization and preliminary X-ray diffraction analysis of the magnesium transporter CorA.

  2006cited by this paper
• Crystal structure of the CorA Mg2+ transporter

  2005cited by this paper
• The CorA Mg2+ Transporter Is a Homotetramer

  2004cited by this paper
• Genes for magnesium transport.

  2003cited by this paper
• Crystal structures of fusion proteins with large‐affinity tags

  2003cited by this paper
• DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli.

  2003cited by this paper
• Two-dimensional assembly of pentameric rabbit C-reactive proteins on lipid monolayers.

  2001cited by this paper
• Role of magnesium in genomic stability.

  2001cited by this paper
• Magnesium. An update on physiological, clinical and analytical aspects.

  2000cited by this paper
• Cation Hexaammines Are Selective and Potent Inhibitors of the CorA Magnesium Transport System*

  2000cited by this paper
• Membrane transport : a practical approach

  2000cited by this paper
• Regulation of cellular magnesium.

  2000cited by this paper
• The CorA Mg2+ Transport Protein of Salmonella typhimurium

  1999cited by this paper
• Pentameric two-dimensional crystallization of rabbit C-reactive protein on lipid monolayers.

  1999cited by this paper
• EMAN: semiautomated software for high-resolution single-particle reconstructions.

  1999cited by this paper
• Magnesium and the Role of mgtC in Growth of Salmonella typhimurium

  1998cited by this paper
• The CorA Mg2+ Transport Protein of Salmonella typhimurium

  1998cited by this paper
• Microbial magnesium transport: unusual transporters searching for identity

  1998cited by this paper
• The CorA Magnesium Transporter Gene Family

  1998cited by this paper
• Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.

  1996cited by this paper
• MRC image processing programs.

  1996cited by this paper
• Magnesium transport in Salmonella typhimurium: mgtA encodes a P-type ATPase and is regulated by Mg2+ in a manner similar to that of the mgtB P-type ATPase

  1995cited by this paper
• Cloning of the mgtE Mg2+ transporter from Providencia stuartii and the distribution of mgtE in gram-negative and gram-positive bacteria

  1995cited by this paper
• Distribution of the CorA Mg2+ transport system in gram-negative bacteria

  1995cited by this paper
• Cloning and characterization of MgtE, a putative new class of Mg2+ transporter from Bacillus firmus OF4

  1995cited by this paper
• Magnesium transport systems: genetics and protein structure (a review).

  1994cited by this paper
• Analysis of electron microscope images and electron diffraction patterns of thin crystals of phi 29 connectors in ice.

  1994cited by this paper
• Molecular aspects of Mg2+ transport systems

  1993cited by this paper
• Sequence and topology of the CorA magnesium transport systems of Salmonella typhimurium and Escherichia coli. Identification of a new class of transport protein.

  1993cited by this paper
• Molecular aspects of Mg2+ transport systems.

  1993cited by this paper
• Cell magnesium transport and homeostasis: role of intracellular compartments.

  1993cited by this paper
• MgtA and MgtB: Prokaryotic P-type ATPases that mediate Mg2+ influx

  1992cited by this paper
• Magnesium transport in Salmonella typhimurium: 28Mg2+ transport by the CorA, MgtA, and MgtB systems

  1989cited by this paper
• Magnesium ions in cardiac function. Regulator of ion channels and second messengers.

  1989cited by this paper
• Magnesium as a regulatory cation: criteria and evaluation.

  1987cited by this paper
• Magnesium transport in Salmonella typhimurium: characterization of magnesium influx and cloning of a transport gene

  1986cited by this paper
• Magnesium and cell cycle control: an update.

  1986cited by this paper
• Two-dimensional crystallization technique for imaging macromolecules, with application to antigen–antibody–complement complexes

  1983cited by this paper
• Magnesium transport in Escherichia coli. Inhibition by cobaltous ion.

  1971cited by this paper
• Materials and Methods Figs. S1 to S3 References and Notes Global Topology Analysis of the Escherichia Coli Inner Membrane Proteome

  year unknowncited by this paper

• Pioneers: Academic Career in Cryo-EM Structural Biology.

  2026cites this paper
• Novel QTL for Low Seed Cadmium Accumulation in Soybean

  2022cites this paper
• Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis.

  2021cites this paper
• Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli.

  2017cites this paper
• High-throughput laboratory evolution of Escherichia coli under multiple stress environments

  2017cites this paper
• Iron Uptake And Accumulation Is A Target Of Nickel Toxicity During The Lag Phase In Escherichia Coli

  2016cites this paper
• The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions

  2015cites this paper
• The structure and regulation of magnesium selective ion channels.

  2013cites this paper
• Discriminating among metals

  2013cites this paper
• The role of nickel in the survival of the basidiomycete fungus Cryptococcus neoformans

  2012cites this paper
• Co2+ Selectivity of Thermotoga maritima CorA and Its Inability to Regulate Mg2+ Homeostasis Present a New Class of CorA Proteins*

  2011cites this paper
• In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p

  2011cites this paper
• Molecular identification of ancient and modern mammalian magnesium transporters.

  2010cites this paper
• Biochemistry of Magnesium

  2009cites this paper
• Interaction of beta-amyloid(1-40) peptide with pairs of metal ions: An electrospray ion trap mass spectrometric model study.

  2009cites this paper
• Understanding structure and function of membrane protein transporters

  2008cites this paper
• Characterization of a CorA Mg2+ transport channel from Methanococcus jannaschii using a Thermofluor-based stability assay

  2008influential citation
• AtMGT7: An Arabidopsis gene encoding a low-affinity magnesium transporter.

  2008cites this paper
• The CorA family: Structure and function revisited

  2007cites this paper