Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68

The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.

• Publication year

  2014

• Venue

  Brazilian Journal of Microbiology

• Publication date

  2014-08-29

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1590/S1517-83822014000200010PMID 25242926PMCID 4166267
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• All the content of the journal, except where otherwise noted, is licensed under a Creative

  2014cited by this paper
• Statistical approach to optimization of fermentative production of oil and arachidonic acid from Mortierella alpine CBS 754.68

  2012cited by this paper
• Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina.

  2009cited by this paper
• BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Identification of a novel fatty acid elongase with a wide substrate specificity from arachidonic acid-producing fungus Mortierella alpina 1S-4

  2009influential reference
• [Production of functional lipids by microorganisms: arachidonic acid and related polyunsaturated fatty acids].

  2009cited by this paper
• Susceptibility to peroxidation of the major mycelial lipids of Cunninghamella echinulata

  2008cited by this paper
• Multiple isoforms of malic enzyme in the oleaginous fungus, Mortierella alpina.

  2008cited by this paper
• Metabolic flux analysis on arachidonic acid fermentation

  2007cited by this paper
• Phenolic compounds, organic acids profiles and antioxidative properties of beefsteak fungus (Fistulina hepatica).

  2007cited by this paper
• Metabolic flux analysis on arachidonic acid fermentation

  2007cited by this paper
• Sclerotial metamorphosis in filamentous fungi is induced by oxidative stress.

  2006cited by this paper
• Optimization of arachidonic acid production by fed-batch culture of Mortierella alpina based on dynamic analysis

  2006cited by this paper
• Lipids of selected molds grown for production of n−3 and n−6 polyunsaturated fatty acids

  2006cited by this paper
• Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina.

  2005cited by this paper
• Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple‐limited media

  2004cited by this paper
• Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.

  2004cited by this paper
• Growth-Coupled Lipid Synthesis in Mortierella alpina LPM 301, a Producer of Arachidonic Acid

  2002cited by this paper
• Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel

  2002cited by this paper
• Wine Phenolics

  2002cited by this paper
• Evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of the 18:3 to 20:3 elongase.

  2000cited by this paper
• The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi.

  1999cited by this paper
• Production of high yields of arachidonic acid in a fed-batch system by Mortierella alpina ATCC 32222

  1997cited by this paper
• Synthesis of γ-linolenic acid by mucoraceous fungi utilizing exogenous fatty acids

  1997cited by this paper
• Production of γ‐Linolenic Acid by the Fungus Cunninghamella echinulata CCRC 31840

  1996cited by this paper
• Production of γ-linolenic acid by the fungus Mucor rouxii in fed-batch and continuous culture

  1989cited by this paper
• Effect of culture conditions on mycelial growth and production of γ-linolenic acid by the fungus Mortierella ramanniana

  1988cited by this paper
• Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis.

  1966cited by this paper
• Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

  1959cited by this paper
• A simple method for the isolation and purification of total lipides from animal tissues.

  1957cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Interrelationship among substrate utilization, metabolic productions, and housekeeping-related gene expression levels in Mortierella alpine CBS 754.68.

  2024cites this paper
• Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production.

  2022cites this paper
• Optimization of culture conditions for enhanced Dunaliella salina productions in mixotrophic culture

  2021cites this paper
• Statistical Approach for Production of Lipid from a Newly-isolated Wickerhamomyces Siamensis SAKSG Strain from Trout Fish

  2018cites this paper
• Effect of low-cost substrate on the fatty acid profiles of Mortierella alpina CBS 754.68 and Wickerhamomyces siamensis SAKSG

  2018cites this paper
• Production of cross-kingdom oxylipins by pathogenic fungi: An update on their role in development and pathogenicity

  2016cites this paper