Phylogenetic inferences of Nepenthes species in Peninsular Malaysia revealed by chloroplast (trnL intron) and nuclear (ITS) DNA sequences

BackgroundThe chloroplastic trnL intron and the nuclear internal transcribed spacer (ITS) region were sequenced for 11 Nepenthes species recorded in Peninsular Malaysia to examine their phylogenetic relationship and to evaluate the usage of trnL intron and ITS sequences for phylogenetic reconstruction of this genus.ResultsPhylogeny reconstruction was carried out using neighbor-joining, maximum parsimony and Bayesian analyses. All the trees revealed two major clusters, a lowland group consisting of N. ampullaria, N. mirabilis, N. gracilis and N. rafflesiana, and another containing both intermediately distributed species (N. albomarginata and N. benstonei) and four highland species (N. sanguinea, N. macfarlanei, N. ramispina and N. alba).ConclusionsThe trnL intron and ITS sequences proved to provide phylogenetic informative characters for deriving a phylogeny of Nepenthes species in Peninsular Malaysia. To our knowledge, this is the first molecular phylogenetic study of Nepenthes species occurring along an altitudinal gradient in Peninsular Malaysia.

• Publication year

  2017

• Venue

  BMC Research Notes

• Publication date

  2017-01-26

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s13104-017-2379-1PMID 28126013PMCID 5270352
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Pitcher Plants of the Old World

  2009influential reference
• Genetic diversity and geographical structure of the pitcher plant Nepenthes vieillardii in New Caledonia: A chloroplast DNA haplotype analysis.

  2008influential reference
• Introduction of a Nuclear Marker for Phylogenetic Analysis of Nepenthaceae

  2006influential reference
• Comparative analysis of a translocated copy of the trnK intron in carnivorous family Nepenthaceae.

  2006cited by this paper
• Morphology and nrITS phylogeny of the genus Pinguicula L. (Lentibulariaceae), with special attention to embryo evolution.

  2006cited by this paper
• The Nepenthaceae of the Netherlands Indies

  2006cited by this paper
• PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)]

  2004cited by this paper
• Variation of Pitcher Morphology within Nepenthes vieillardii Hook. f. (Nepenthaceae) in New Caledonia

  2004cited by this paper
• PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10

  2002cited by this paper
• Bayesian Inference of Phylogeny and Its Impact on Evolutionary Biology

  2001cited by this paper
• Inferring the history of the polyploid Silene aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequences.

  2001cited by this paper
• MRBAYES: Bayesian inference of phylogenetic trees

  2001cited by this paper
• Nepenthes of Sumatra and Peninsular Malaysia

  2001influential reference
• Molecular Phylogeny of Nepenthaceae Based on Cladistic Analysis of Plastid trnK Intron Sequence Data

  2001cited by this paper
• PAUP* Phylogenetic analysis using parsimony (*and other methods) Version 4

  2000cited by this paper
• MODELTEST: testing the model of DNA substitution

  1998cited by this paper
• A skeletal revision of Nepenthes (Nepenthaceae)

  1997influential reference
• Bayesian phylogenetic inference using DNA sequences: a Markov Chain Monte Carlo Method.

  1997cited by this paper
• Nepenthes of Borneo

  1997cited by this paper
• Pitcher-plants of Borneo

  1996cited by this paper
• Universal primers for amplification of three non-coding regions of chloroplast DNA

  1991cited by this paper
• Ribosomal DNA variation within and among individuals ofLisianthius (Gentianaceae) populations

  1990cited by this paper

• Studi Filogenetik Kantung Semar (Nepenthes spp.) Berdasarkan Sekuens Gen Nep2 dan trnK di Paparan Sunda: Studi in Silico

  2023influential citation
• DNA Barcoding, Phylogenetic Analysis and Secondary Structure Predictions of Nepenthes ampullaria, Nepenthes gracilis and Nepenthes rafflesiana

  2023cites this paper
• Brief Documentation of Terrestrial Ecosystem in Kubah, Bako, and Santubong National Park, Sarawak

  2022cites this paper
• UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

  2021cites this paper
• Introgression is widespread in the radiation of carnivorous Nepenthes pitcher plants.

  2021cites this paper
• Nepenthes malayensis (Nepenthaceae), a new species of carnivorous pitcher plant from Peninsular Malaysia

  2020influential citation
• Nepenthes latiffiana and N. domei (Nepenthaceae), two new species of pitcher plants from Terengganu, Peninsular Malaysia

  2020cites this paper
• Genome skimming provides well resolved plastid and nuclear phylogenies, showing patterns of deep reticulate evolution in the tropical carnivorous plant genus Nepenthes (Caryophyllales)

  2019cites this paper
• A phylogenomic analysis of Nepenthes (Nepenthaceae)

  2019cites this paper
• Identification and Cluster Analysis of Pitcher Plant (Nepenthes spp.) from South Sumatera Indonesia

  2018cites this paper
• DNA barcoding of the genus Nepenthes (Pitcher plant): a preliminary assessment towards its identification

  2018cites this paper
• Using the EDIT Platform for Cybertaxonomy to prepare and publish a treatment for the Caryophyllales Network: an online synthesis of the Nepenthaceae

  2018cites this paper