On the basis of morphological differences, three subspecies of Black-tailed Godwit (Limosa limosa) have been recognized (L. l. limosa, L. l. islandica and L. l. melanuroides). In previous studies mitochondrial DNA (mtDNA) sequence data showed minimal genetic divergence between the three subspecies and an absence of sub-structuring within L. l. limosa. Here, population genetic structure and phylogeographic patterns have been analyzed using COI, HVR1 and HVR2 mtDNA sequence data as well as 12 microsatellite loci (nuDNA). The nuDNA data suggest genetic differentiation between L. l. limosa from Sweden and The Netherlands, between L. l. limosa and L. l. islandica, but not between L. l. limosa and L. l. melanuroides. However, the mtDNA data were not consistent with the nuDNA pattern. mtDNA did support a split between L. l. melanuroides and L. l. limosa/L. l. islandica and also demonstrated two L. l. limosa haplotype clusters that were not geographically isolated. This genetic structure can be explained by a scenario of isolation of L. l. melanuroides from L. l. limosa in Beringia during the Last Glacial Maximum. During the Pleistocene separation of L. l. islandica from L. l. limosa occurred, followed by colonization of Iceland by the L. l. islandica during the Holocene. Within L. l. limosa founder events, followed by population expansion, took place during the Holocene also. According to the patterns observed in both markers together and their geographic separation, we propose that the three traditional subspecies indeed represent three separate genetic units.
Patterns in Nuclear and Mitochondrial DNA Reveal Historical and Recent Isolation in the Black-Tailed Godwit (Limosa limosa)
K. Trimbos,C. Doorenweerd,K. Kraaijeveld,C. Musters,N. M. Groen,P. de Knijff,T. Piersma,G. D. de Snoo
Published 2014 in PLoS ONE
ABSTRACT
PUBLICATION RECORD
- Publication year
2014
- Venue
PLoS ONE
- Publication date
2014-01-09
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
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CLAIMS
CONCEPTS
- beringia
A high-latitude land region invoked as the geographic setting for historical isolation in the proposed scenario.
- black-tailed godwit (limosa limosa)
A wader species that is the focal taxon examined for population structure and phylogeography in this paper.
Aliases: Black-tailed Godwit, Limosa limosa
- haplotype clusters
Groups of related mtDNA haplotypes identified within L. l. limosa in the sequence data.
Aliases: L. l. limosa haplotype clusters
- holocene
The post-glacial epoch used as the time frame for later diversification and colonization events in the proposed scenario.
- last glacial maximum
The late Pleistocene climatic interval used as the time frame for the proposed isolation of lineages.
Aliases: LGM
- l. l. islandica
An Iceland-associated subspecies of Black-tailed Godwit included in the comparisons among lineages.
Aliases: Limosa limosa islandica
- l. l. limosa
A nominate subspecies of Black-tailed Godwit included in the comparisons among regional populations.
Aliases: Limosa limosa limosa
- l. l. melanuroides
An East Asian/Australasian subspecies of Black-tailed Godwit included in the comparisons among lineages.
Aliases: Limosa limosa melanuroides
- microsatellite loci
A set of 12 nuclear DNA markers used here to test population genetic structure.
Aliases: nuDNA microsatellite loci, microsatellites
- mtdna sequence data
Mitochondrial DNA sequence markers used here to assess phylogeographic patterns across subspecies.
Aliases: mitochondrial DNA sequence data, mtDNA
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