Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen

In general, a whole virion serves as a simple vaccine antigen and often essential material for the analysis of immune responses against virus infection. However, to work with highly contagious pathogens, it is necessary to take precautions against laboratory-acquired infection. We have learned many lessons from the recent outbreak of severe acute respiratory syndrome (SARS). In order to develop an effective vaccine and diagnostic tools, we prepared UV-inactivated SARS coronavirus on a large scale under the strict Biosafety Level 3 (BSL3) regulation. Our protocol for large-scale preparation of UV-inactivated SARS-CoV including virus expansion, titration, inactivation, and ultracentrifugation is applicable to any newly emerging virus we might encounter in the future.

• Publication year

  2007

• Venue

  Methods in molecular biology

• Publication date

  2007-11-28

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/978-1-59745-181-9_11PMID 19057880PMCID 7122600
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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