Immunological methods for mapping protein distributions on polytene chromosomes.

Publisher Summary This chapter focuses on immunological methods for mapping protein distributions on polytene chromosomes. The reproducible banding pattern of the polytene chromosomes has been used to map genes relative to chromosomal rearrangements, to map cloned DNAs to specific bands, to probe chromatin structure, and to map sites of protein accumulation. Polytene chromosomes are created through a process known as endoreduplication, in which multiple rounds of DNA replication occur without the concomitant strand separation and subsequent nuclear and cell division characteristic of normal mitoses. The characteristic polytene-binding pattern has been useful for mapping DNA and chromatin structure. Antibodies have been used to map regions of heterochromatization and atypical DNA structure such as non-B and Z-DNA. Antibody staining of polytene chromosomes has provided evidence for the in vivo association of transcription factors with the genes they regulate and the in vivo association of proteins with other proteins of related function.

• Publication year

  1994

• Venue

  Methods in Cell Biology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0091-679X(08)60923-1PMID 7707963
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The polytene chromosomes in the fat body nuclei ofDrosophila melanogaster

  2005cited by this paper
• The proteins of polytene chromosomes of Drosophila hydei

  2004cited by this paper
• Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome

  2004cited by this paper
• In situ immunofluorescent visualization of chromosomal transcripts in polytene chromosomes

  2004cited by this paper
• Regulation of the sex-specific binding of the maleless dosage compensation protein to the male X chromosome in Drosophila.

  1993cited by this paper
• Related chromosome binding sites for zeste, suppressors of zeste and Polycomb group proteins in Drosophila and their dependence on Enhancer of zeste function.

  1993cited by this paper
• The Polycomb group gene Posterior Sex Combs encodes a chromosomal protein.

  1993cited by this paper
• Single-minded regulation of genes in the embryonic midline of the Drosophila central nervous system.

  1993cited by this paper
• The reaction with polytene chromosomes of antibodies raised against Drosophila E75A protein.

  1993cited by this paper
• Immunochemical probing of DNA structure with monoclonal antibody to OsO4/2,2'-bipyridine adduct.

  1993cited by this paper
• The polyhomeotic gene of Drosophila encodes a chromatin protein that shares polytene chromosome-binding sites with Polycomb.

  1992cited by this paper
• Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster.

  1992cited by this paper
• The Drosophila nuclear protein Bx42, which is found in many puffs on polytene chromosomes, is highly charged

  1992cited by this paper
• The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2.

  1992cited by this paper
• Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites

  1992cited by this paper
• Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster.

  1992cited by this paper
• Dorsoventral development of the Drosophila embryo is controlled by a cascade of transcriptional regulators.

  1992cited by this paper
• The maternally expressed Drosophila gene encoding the chromatin‐binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1.

  1991cited by this paper
• Drosophila snRNP associated protein P11 which specifically binds to heat shock puff 93D reveals strong homology with hnRNP core protein A1.

  1991cited by this paper
• The regulation of the cell cycle during Drosophila embryogenesis: the transition to polyteny.

  1991cited by this paper
• Genomic targets of the serendipity beta and delta zinc finger proteins and their respective DNA recognition sites.

  1991cited by this paper
• Distribution of chromosomal proteins in polytene chromosomes of Drosophila.

  1991cited by this paper
• The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila.

  1991cited by this paper
• The Polycomb protein shares a homologous domain with a heterochromatin-associated protein of Drosophila.

  1991cited by this paper
• Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin.

  1991cited by this paper
• A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila.

  1991cited by this paper
• Direct interaction of the Polycomb protein with Antennapedia regulatory sequences in polytene chromosomes of Drosophila melanogaster.

  1991cited by this paper
• Molecular interactions within the ecdysone regulatory hierarchy: DNA binding properties of the Drosophila ecdysone-inducible E74A protein.

  1990cited by this paper
• Chromosome structure at interfaces between major chromatin types: Alpha‐ and Beta‐heterochromatin

  1990cited by this paper
• Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster.

  1990cited by this paper
• The closely related Drosophila sry beta and sry delta zinc finger proteins show differential embryonic expression and distinct patterns of binding sites on polytene chromosomes.

  1990cited by this paper
• Two puff-specific proteins bind within the 2.5 kb upstream region of theDrosophila melanogaster Sgs-4 gene

  1990cited by this paper
• In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster

  1989cited by this paper
• Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila

  1989cited by this paper
• Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila.

  1989cited by this paper
• Isolation and distribution of a Drosophila protein preferentially associated with active regions of the genome

  1989cited by this paper
• Developmental expression of the Drosophila zeste gene and localization of zeste protein on polytene chromosomes.

  1988cited by this paper
• Expression of the Sex combs reduced protein in Drosophila larvae.

  1988cited by this paper
• Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method

  1988cited by this paper
• Multiple forms and cellular localization of Drosophila DNA topoisomerase II.

  1986cited by this paper
• Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene

  1986cited by this paper
• Locations of Z-DNA in polytene chromosomes

  1985cited by this paper
• Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70.

  1984cited by this paper
• Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.

  1984cited by this paper
• Nonpackaging and packaging proteins of hnRNA in Drosophila melanogaster.

  1983cited by this paper
• Left-handed Z-DNA in bands of acid-fixed polytene chromosomes.

  1983cited by this paper
• Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

  1981cited by this paper
• Distribution studies on polytene chromosomes using antibodies directed against hnRNP

  1981cited by this paper
• Immunological identification of a chromocenter-associated protein in polytene chromosomes of Drosophila.

  1980cited by this paper
• Monoclonal antibodies against chromosomal proteins of Drosophila melanogaster

  1980cited by this paper
• A protein released by DNAase I digestion of drosophila nuclei is preferentially associated with puffs.

  1978cited by this paper
• RNA Polymerase B (or II) in heat induced puffs of Drosophila polytene chromosomes

  1978cited by this paper
• Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster.

  1977cited by this paper
• Distribution patterns of three subfractions of drosophila nonhistone chromosomal proteins: possible correlations with gene activity.

  1977cited by this paper
• Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence

  1976cited by this paper
• A method for determination of the in situ distribution of chromosomal proteins.

  1976cited by this paper
• Locations of chromosomal proteins in polytene chromosomes.

  1976cited by this paper
• Structural repeating units in chromatin. I. Evidence for their general occurrence.

  1976cited by this paper
• The effect of heat shock on RNA synthesis in Drosophila tissues.

  1976cited by this paper
• Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs.

  1974cited by this paper
• Immunofluorescent labeling of chromosomes with antisera to histones and histone fractions.

  1972cited by this paper
• A NEW MAP OF THE SECOND CHROMOSOMEA Revised Map of the Right Limb of the Second Chromosome of Drosophila Melanogaster

  1939cited by this paper
• A REVISED MAP OF THE SALIVARY GLAND X-CHROMOSOME of Drosophila Melanogaster

  1938cited by this paper
• SALIVARY CHROMOSOME MAPSWith a Key to the Banding of the Chromosomes of Drosophila Melanogaster

  1935cited by this paper

• High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.

  2020cites this paper
• Diverse patterns of genomic targeting by transcriptional regulators in Drosophila melanogaster

  2014cites this paper
• Drosophila melanogaster in Diverse patterns of genomic targeting by transcriptional regulators Material Supplemental

  2014cites this paper
• Organ-specific gene expression: the bHLH protein Sage provides tissue specificity to Drosophila FoxA

  2013cites this paper
• Genome-Wide Analysis Reveals a Major Role in Cell Fate Maintenance and an Unexpected Role in Endoreduplication for the Drosophila FoxA Gene Fork Head

  2011cites this paper
• Staying alive: Dalmatian mediated blocking of apoptosis is essential for tissue maintenance

  2010cites this paper
• Functional interactions between the Moses corepressor and DHR78 nuclear receptor regulate growth in Drosophila.

  2007cites this paper
• The Drosophila ramshackle gene encodes a chromatin-associated protein required for cell morphology in the developing eye.

  2006cites this paper
• DSP1, a Drosophila HMG protein, is involved in spatiotemporal expression of the homoeotic gene Sex combs reduced

  2005cites this paper
• Genetic and cytological analysis of Drosophila chromatin-remodeling factors.

  2004cites this paper
• Hairy Transcriptional Repression Targets and Cofactor Recruitment in Drosophila

  2004cites this paper
• Differential expression of the Drosophila BX-C in polytene chromosomes in cells of larval fat bodies: a cytological approach to identifying in vivo targets of the homeotic Ubx, Abd-A and Abd-B proteins

  2003cites this paper
• Localizing transcription factors on chromatin by immunofluorescence.

  2002cites this paper
• Bonus, a Drosophila homolog of TIF1 proteins, interacts with nuclear receptors and can inhibit betaFTZ-F1-dependent transcription.

  2001cites this paper
• The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family.

  2001cites this paper
• Covalent Modification of the Transcriptional Repressor Tramtrack by the Ubiquitin-Related Protein Smt3 inDrosophila Flies

  2000cites this paper
• Regulation of invasive cell behavior by taiman, a Drosophila protein related to AIB1, a steroid receptor coactivator amplified in breast cancer.

  2000cites this paper
• Covalent Modification of the Transcriptional Repressor Tramtrack by the Ubiquitin-Related Protein Smt3 in Drosophila Flies

  1999cites this paper
• The Zw5 protein, a component of the scs chromatin domain boundary, is able to block enhancer-promoter interaction.

  1999cites this paper
• The DHR78 nuclear receptor is required for ecdysteroid signaling during the onset of Drosophila metamorphosis.

  1998cites this paper
• DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster

  1998cites this paper