Purification and characterization of an estrogen-inducible membrane glycoprotein. Evidence that it is a transferrin receptor.

A number of N-linked membrane glycoproteins are induced during chick oviduct differentiation. We have purified a major estrogen-inducible glycoprotein (Mr = 91,000) to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparison of partial NH2-terminal sequence data with membrane glycoproteins having similar Mr showed a limited homology with human and murine transferrin receptors. We observed that oviduct membranes contain estrogen-inducible transferrin receptor activity (Kd = 2-8 x 10(-8) M). Analytical purification of the putative receptor on an ovotransferrin-Affi-Gel affinity column and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis reveals a protein of Mr, 180,000, which contains two disulfide-linked subunits of Mr 91,000. The receptor reacts very strongly with antibodies prepared against the 91-kDa glycoprotein on Western blots. Western blot analysis confirms that the 91-kDa glycoprotein is induced by estrogen. The protein has 2% total carbohydrate with Man, GlcNAc, Gal, GalNAc, and NeuAc in a molar ratio of 6:4:2:1:1. The protein contains at least one O-linked moiety. Analysis of the O-linked moiety by glycosidase digestions and gel filtration indicates there are sialo tetra- and trisaccharides and a neutral disaccharide(s). Labeled N-linked glycopeptides were prepared by pronase digestion, beta-elimination, and 3H-acetylation. The N-linked oligosaccharides include high mannose and complex neutral nonbisected biantennary types in an approximate ratio of 3:1 as determined by serial lectin affinity chromatography.

• Publication year

  1988

• Venue

  Journal of Biological Chemistry

• Publication date

  1988-12-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)37401-5PMID 3198616
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Characterization of the transferrin receptor in tunicamycin-treated A431 cells.

  1988cited by this paper
• Insulin-like growth factor II receptor as a multifunctional binding protein

  1987cited by this paper
• The physiology of transferrin and transferrin receptors.

  1987cited by this paper
• Identification of the intermolecular disulfide bonds of the human transferrin receptor and its lipid‐attachment site.

  1987cited by this paper
• Improved purification of the human placental transferrin receptor and a novel immunoradiometric assay for receptor protein.

  1986cited by this paper
• Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17 alpha-ethinyl estradiol.

  1986cited by this paper
• Identification and characterization of the chicken transferrin receptor.

  1985cited by this paper
• Regulation of dolichyl phosphate-mediated protein glycosylation: estrogen effects on glucosyl transfers in oviduct membranes.

  1985cited by this paper
• Assembly of asparagine-linked oligosaccharides.

  1985cited by this paper
• Regulation of epidermal growth factor receptor by estrogen.

  1985cited by this paper
• Gene transfer, expression, and molecular cloning of the human transferrin receptor gene.

  1984cited by this paper
• Promoter elements of genes coding for proteins and modulation of transcription by estrogens and progesterone.

  1984cited by this paper
• A recommendation for visualizing disulfide bonding by one-dimensional sodium dodecyl sulfate--polyacrylamide gel electrophoresis.

  1984cited by this paper
• Transferrin receptors: structure and function.

  1984cited by this paper
• Yeast RNA polymerase II genes: isolation with antibody probes.

  1983cited by this paper
• Structural studies of the sugar chains of hen ovomucoid. Evidence indicating that they are formed mainly by the alternate biosynthetic pathway of asparagine-linked sugar chains.

  1983cited by this paper
• Visualization of lipoprotein receptors by ligand blotting.

  1983cited by this paper
• Placental transferrin receptor. Evaluation of the presence of endogenous ligand on specific binding.

  1983cited by this paper
• Stimulation of lipid-linked oligosaccharide assembly during oviduct differentiation.

  1983cited by this paper
• A dot-immunobinding assay for monoclonal and other antibodies.

  1982cited by this paper
• Fractionation of asparagine-linked oligosaccharides by serial lectin-Agarose affinity chromatography. A rapid, sensitive, and specific technique.

  1982cited by this paper
• Structural features of the cell surface receptor for transferrin that is recognized by the monoclonal antibody OKT9.

  1982cited by this paper
• Estrogen-induced changes in chick oviduct membrane glycoproteins.

  1982cited by this paper
• Fluorometric analysis of amino sugars and derivatized neutral sugars.

  1982cited by this paper
• Increased transfer of oligosaccharide from oligosaccharide pyrophosphoryl dolichol to protein acceptors upon estrogen-induced chick oviduct differentiation.

  1981cited by this paper
• Radioimmunochemical measurement of the transferrin receptor in human trophoblast and reticulocyte membranes with a specific anti-receptor antibody.

  1981cited by this paper
• The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant.

  1981cited by this paper
• Structural requirements for the binding of oligosaccharides and glycopeptides to immobilized wheat germ agglutinin.

  1981cited by this paper
• Sequestration of 125I-labeled beta nerve growth factor by sympathetic neurons.

  1980cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Effect of estrogen on gene expression in chicken oviduct: evidence for transcriptional control of ovalbumin gene.

  1979cited by this paper
• Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.

  1979cited by this paper
• Structures of the carbohydrate moiety of ovalbumin glycopeptide III and the difference in specificity of endo-beta-N-acetylglucosaminidases CII and H.

  1977cited by this paper
• Increase in the lipid intermediate pathway of protein glycosylation during hen oviduct differentiation.

  1977cited by this paper
• Assay of proteins in the presence of interfering materials.

  1976cited by this paper
• Approaches to the standardization of serum unsaturated iron-binding capacity.

  1976cited by this paper
• Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.

  1975cited by this paper
• High-resolution preparative SDS-polyacrylamide gel electrophoresis: fluorescent visualization and electrophoretic elution-concentration of protein bands.

  1975cited by this paper
• Structure of the O-glycosidically linked carbohydrate units of fetuin.

  1974cited by this paper
• Endo-beta-N-acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from Diplococcus pneumoniae.

  1974cited by this paper
• Membrane glycoproteins. I. Enzymatic synthesis of mannosyl phosphoryl polyisoprenol and its role as a mannosyl donor in glycoprotein synthesis.

  1973cited by this paper
• Fluorescent monitoring of SDS gel electrophoresis.

  1971cited by this paper
• The complete amino acid sequence of bovine alpha-lactalbumin.

  1970cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• INTERACTION OF ESTROGEN AND PROGESTERONE IN CHICK OVIDUCT DEVELOPMENT

  1969cited by this paper
• Spectroscopic determination of tryptophan and tyrosine in proteins.

  1967cited by this paper
• The thiobarbituric acid assay of sialic acids.

  1959cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Estropause, Sex Hormones and Metal Homeostasis in the Mouse Brain

  2022cites this paper
• Molecular evolution of the transferrin family and associated receptors.

  2012cites this paper
• Physiological roles of ovotransferrin.

  2012cites this paper
• Transient Expression of Iron Transport Proteins in the Capillary of the Developing Rat Brain

  2010cites this paper
• A comparative study of the antiproliferative activity of iron chelators PIH, SIH and their light-activated caged derivatives in skin cells

  2010cites this paper
• Heterogeneity of Glia in the Retina and Optic Nerve of Birds and Mammals

  2010cites this paper
• The expression of transferrin binding protein in the turtle nervous system.

  2009cites this paper
• Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription

  2008cites this paper
• Expression of Transferrin Binding Protein in the Capillaries of the Brain in the Developing Chick Embryo

  2008influential citation
• Developmental Expression of Transferrin Binding Protein in Oligodendrocyte Lineage Cells of the Embryonic Chick Spinal Cord

  2006cites this paper
• Altered cellular distribution of iron in rat cerebral cortex during the oestrous cycle

  2004cites this paper
• Heat shock protein 108 mRNA expression during chicken retina development.

  2003cites this paper
• Evolution of heat shock protein and immunity.

  2003cites this paper
• The iron metabolism of neoplastic cells: alterations that facilitate proliferation?

  2002cites this paper
• Glycan‐dependent signaling: O‐linked N‐acetylglucosamine

  2001cites this paper
• Role of iron in estrogen-induced cancer.

  2001cites this paper
• Oligodendroglia in the avian retina: Immunocytochemical demonstration in the adult bird

  2001cites this paper
• Estrogen Modulates HNF-3 beta mRNA Levels in the Developing Chick Oviduct

  2000cites this paper
• Transferrin binding protein is expressed by oligodendrocytes in the avian retina.

  1999cites this paper
• Expression of neuron-specific enolase, chromogranin A, synaptophysin and Leu-7 in lung cancer cell lines.

  1998cites this paper
• Localization of transferrin binding protein in relation to iron, ferritin, and transferrin receptors in the chicken cerebellum.

  1998cites this paper
• Associate Editor: D. Shugar The 90-kDa Molecular Chaperone Family: Structure, Function, and Clinical Applications. A Comprehensive Review

  1998cites this paper
• An estrogen inducible 104 kDa chaperone glycoprotein binds ferric iron containing proteins: a possible role in intracellular iron trafficking

  1997cites this paper
• Binding specificity of avian heat shock protein 108.

  1997cites this paper
• Distribution of transferrin binding protein immunoreactivity in the chicken central and peripheral nervous systems

  1997cites this paper
• An immunohistochemical localization of transferrin binding protein in the chicken respiratory tract.

  1996cites this paper
• Immunocytochemical study with an anti-transferrin binding protein serum: a marker for avian oligodendrocytes.

  1995cites this paper
• A chicken transferrin binding protein is heat shock protein 108.

  1994cites this paper
• The estrogen-inducible transferrin receptor-like membrane glycoprotein is related to stress-regulated proteins.

  1994cites this paper
• Ovotransferrin and ovotransferrin receptor expression during chondrogenesis and endochondral bone formation in developing chick embryo

  1994cites this paper
• Identification of the O-linked glycosylation site of the human transferrin receptor.

  1992cites this paper
• Studies of the mechanism of iron transport across the blood‐brain barrier

  1992cites this paper
• Differential tissue localization of oviduct and erythroid transferrin receptors.

  1991cites this paper
• The ontogeny of transferrin receptors in the embryonic chick retina: an immunohistochemical study.

  1991cites this paper
• Studies on the mechanism of action of oral contraceptives with regard to fibrinolytic variables.

  1990cites this paper
• Human transferrin receptor contains O-linked oligosaccharides.

  1990cites this paper
• The chicken oviduct and embryonic red blood cell transferrin receptors are distinct molecules.

  1990cites this paper