Immunofluorescence in the study of Marek's disease: detection of antibody.

H. Purchase,G. H. Burgoyne

Published 1970 in American Journal of Veterinary Research

ABSTRACT

Indirect fluorescent antibody (fa) and agar gel precipitin (agp) tests were used to detect antibody in serums of chickens which were exposed to Marek's disease (md) virus. There was a 92% agreement between the results of the fa and those of the agp tests on 418 serums from various sources. By the fa test, maternal antibody in young chickens from md-exposed dams was detected after antibody was no longer detectable by the agp test, and acquired antibody in contact-exposed chickens was detected earlier than by the agp test. The fa test was 10 to more than 320 times more sensitive than the agp test; also, some serums which had a high titer of antibody demonstrable by the fa test did not produce precipitation in the agp test.

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