Purification and properties of the inducible enzyme cyanase.

No abstract is available for this paper.

• Publication year

  1980

• Venue

  Biochemistry

• Publication date

  1980-06-24

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00554A010PMID 6994799
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cyanate modification of essential lysyl residues in the catalytic subunit of tobacco ribulosebisphosphate carboxylase.

  1978cited by this paper
• The measurement of partial specific volumes and sedimentation coefficients by sucrose density centrifugation.

  1978cited by this paper
• Isolation and properties of the rabbit skeletal muscle protein inhibitor of adenosine 3',5'-monophosphate dependent protein kinases.

  1977cited by this paper
• Hydrolysis of cyanate catalyzed by guinea pig and rat tissue extracts.

  1977cited by this paper
• Complete amino acid analysis of proteins from a single hydrolysate.

  1976cited by this paper
• A view of the current status of antisickling therapy

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• A thermochemical study of the hydrolysis of urea by urease.

  1975cited by this paper
• Cyanate modification of essential lysyl residues of the diphosphopyridine nucleotide-specific isocitrate dehydrogenase of pig heart.

  1975cited by this paper
• A THERMOCHEMICAL STUDY OF THE HYDROLYSIS OF UREA BY UREASE

  1975cited by this paper
• Reversible reaction of cyanate with a reactive sulfhydryl group at the glutamine binding site of carbamyl phosphate synthetase.

  1975cited by this paper
• Functional groups of diphosphopyridine nucleotide linked isocitrate dehydrogenase from bovine heart. I. Studies of an active amino group by amidination, arylation, acetylation, and carbamylation.

  1974cited by this paper
• Use of narrow-bore columns in amino acid analysis

  1973cited by this paper
• Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

  1972cited by this paper
• [37] Reaction of protein sulfhydryl groups with Ellman's reagent.

  1972cited by this paper
• Biosynthesis of the purines. XXXII. Effect of albizziin and other reagents on the activity of formylglycinamide ribonucleotide amidotransferase.

  1969cited by this paper
• Jack bean urease (EC 3.5.1.5). Demonstration of a carbamoyl-transfer reaction and inhibition by hydroxamic acids.

  1969cited by this paper
• Reversible inactivation of papain by cyanate.

  1967cited by this paper
• Studies of the esterase activity and the anion inhibition of bovine zinc and cobalt carbonic anhydrases.

  1967cited by this paper
• Spectroscopic determination of tryptophan and tyrosine in proteins.

  1967cited by this paper
• Some properties of the induced enzyme cyanase.

  1965cited by this paper
• The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

  1965cited by this paper
• DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS *

  1964cited by this paper
• DECOMPOSITION OF CARBAMYLPHOSPHATE IN AQUEOUS SOLUTIONS.

  1964cited by this paper
• DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.

  1964cited by this paper
• On the determination of cystine as cysteic acid.

  1963cited by this paper
• [117] Chromatographic determination of amino acids by the use of automatic recording equipment

  1963cited by this paper
• A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

  1961cited by this paper
• CHEMICAL AND ENZYMATIC SYNTHESIS OF CARBAMYL PHOSPHATE.

  1960cited by this paper
• The synthesis of the induced enzyme, ''cyanase'', in E. coli.

  1960cited by this paper
• On the mechanism of urease action.

  1959cited by this paper
• Simple Microdetermination of Kjeldahl Nitrogen in Biological Materials

  1958cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper
• Cyanase, a new enzyme catalysing the hydrolysis of cyanate

  1948cited by this paper
• A NEW DIRECT NESSLERIZATION MICRO-KJELDAHL METHOD AND A MODIFICATION OF THE NESSLER-FOLIN REAGENT FOR AMMONIA

  1924cited by this paper

• Detoxifying Cyanides Using Cyanase Enzyme Complexes Composed of Carbonic Anhydrase via Irreversible Covalent Bonds.

  2024cites this paper
• Main group cyanides: from hydrogen cyanide to cyanido-complexes

  2022cites this paper
• Heterologous Expression of Cyanobacterial Cyanase Gene (CYN) in Microalga Chlamydomonas reinhardtii for Bioremediation of Cyanide Pollution

  2022cites this paper
• Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation

  2021cites this paper
• Efficiency of transgenic Chlamydomonas reinhardtii for removing of toxicity of cyanide compound

  2021cites this paper
• Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation

  2021cites this paper
• Isolation of cyanide‐degrading bacteria and molecular characterization of its cyanide‐degrading nitrilase

  2020cites this paper
• Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster

  2019cites this paper
• Overexpression of Cyanase in Chlamydomonas reinhardtii: a Promising Approach for Biodegradation of Cyanate in Aquatic Systems

  2019cites this paper
• Cyclization mechanism catalyzed by an ATP‐grasp enzyme essential for d‐cycloserine biosynthesis

  2019cites this paper
• Enzymatic cyanide detoxification by partially purified cyanide dihydratase obtained from Serratia marcescens strain AQ07.

  2019influential citation
• Expression of a novel recombinant cyanate hydratase (rTl-Cyn) in Pichia pastoris, characteristics and applicability in the detoxification of cyanate.

  2017cites this paper
• Expression of the cyanobacterial enzyme cyanase increases cyanate metabolism and cyanate tolerance in Arabidopsis

  2017cites this paper
• Structural and Functional Studies of Proteins from the Agricultural Pests Tetranychus Urticae and Aspergillus Fumigatus

  2017cites this paper
• Structural Characterization of a Eukaryotic Cyanase from Tetranychus urticae.

  2017cites this paper
• Characterization of an autotrophic bioreactor microbial consortium degrading thiocyanate

  2017cites this paper
• New insights into the genetic and metabolic diversity of thiocyanate-degrading microbial consortia

  2015cites this paper
• New insights into the genetic and metabolic diversity of thiocyanate-degrading 1 consortia

  2015cites this paper
• New insights into the genetic and metabolic diversity of thiocyanate-degrading 1 consortia

  2015cites this paper
• The role of horizontally transferred genes in the xenobiotic adaptations of the spider mite Tetranychus urticae

  2015cites this paper
• Enzymatic study of cyanide utilizing Pseudomonas species isolated from contaminated soil

  2013cites this paper
• Cyanamide in plant metabolism

  2013cites this paper
• Distribution and expression of the cyanate acquisition potential among cyanobacterial populations in oligotrophic marine waters

  2013influential citation
• A horizontally transferred cyanase gene in the spider mite Tetranychus urticae is involved in cyanate metabolism and is differentially expressed upon host plant change.

  2012cites this paper
• Biochemical and Structural Properties of Cyanases from Arabidopsis thaliana and Oryza sativa

  2011cites this paper
• Characterization of Cyanate Metabolism in Marine Synechococcus and Prochlorococcus spp

  2010influential citation
• Nitrite Transport Activity of the ABC-Type Cyanate Transporter of the Cyanobacterium Synechococcus elongatus

  2009cites this paper
• A cyanase is transcriptionally regulated by arginine and involved in cyanate decomposition in Sordaria macrospora.

  2008cites this paper
• Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an Enzyme That Is Not Essential for Cyanide Assimilation

  2008cites this paper
• Thiocyanate hydrolase, the primary enzyme initiating thiocyanate degradation in the novel obligately chemolithoautotrophic halophilic sulfur-oxidizing bacterium Thiohalophilus thiocyanoxidans.

  2007cites this paper
• Involvement of the cynABDS Operon and the CO2-Concentrating Mechanism in the Light-Dependent Transport and Metabolism of Cyanate by Cyanobacteria

  2006influential citation
• Bacterial Degradation of Cyanide and Its Metal Complexes under Alkaline Conditions

  2005cites this paper
• Alkaline cyanide biodegradation by Pseudomonas pseudoalcaligenes CECT5344.

  2005cites this paper
• THE BIOGEOCHEMISTRY OF CALCRETE FORMING PROCESSES

  2004cites this paper
• Flow-injection spectrophotometric determination of cyanate in bioremediation processes by use of immobilised inducible cyanase

  2003cites this paper
• Microbial Thiocyanate Utilization under Highly Alkaline Conditions

  2001influential citation
• Prokaryotic carbonic anhydrases.

  2000cites this paper
• Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site.

  2000cites this paper
• Bacterial carbonic anhydrases.

  2000cites this paper
• Sodium cyanate alters glutathione homeostasis in rodent brain: relationship to neurodegenerative diseases in protein-deficient malnourished populations in Africa.

  1999cites this paper
• Fate and transformation of thiocyanate and cyanate under methanogenic conditions

  1998cites this paper
• Biodegradation of cyanides, cyanates and thiocyanates to ammonia and carbon dioxide by immobilized cells of Pseudomonas putida

  1998cites this paper
• A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources

  1998influential citation
• Aerobic biodegradation of thiocyanate

  1997cites this paper
• Cell-free extract(s) of Pseudomonas putida catalyzes the conversion of cyanides, cyanates, thiocyanates, formamide, and cyanide-containing mine waters into ammonia

  1996cites this paper
• Cyanide degradation by an Escherichia coli strain.

  1996cites this paper
• Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate

  1995cites this paper
• Purification and properties of two malic enzyme activities in liver mitochondria of Squalus acanthias (spiny dogfish)

  1995cites this paper
• Photosynthetic metabolism of cyanate by the cyanobacterium Synechococcus UTEX 625

  1994influential citation
• Reaction of the N-terminal methionine residues in cyanase with diethylpyrocarbonate.

  1994cites this paper
• Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation

  1994cites this paper
• Formation of intersubunit disulfide bonds and properties of the single histidine and cysteine residues in each subunit relative to the decameric structure of cyanase.

  1994cites this paper
• Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase

  1994cites this paper
• Sustaining Protein Synthesis in the Absence of Rapid Cell Division: An Investigation of Plasmid‐Encoded Protein Expression in Escherichia coli during Very Slow Growth

  1993cites this paper
• Expression and purification of the cynR regulatory gene product: CynR is a DNA-binding protein

  1993cites this paper
• A physiological role for cyanate-induced carbonic anhydrase in Escherichia coli

  1993cites this paper
• Biochemischer Abbau von Cyanamid und Dicyandiamid

  1992cites this paper
• Carbonic anhydrase in Escherichia coli. A product of the cyn operon.

  1992cites this paper
• The inhibition of rat liver threonine dehydratase by carbamoyl-phosphate. The formation of carbamoylpyridoxal 5'-phosphate.

  1991cites this paper
• Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene.

  1991cites this paper
• The cyanase operon and cyanate metabolism.

  1990cites this paper
• Cyanase-mediated utilization of cyanate in Pseudomonas fluorescens NCIB 11764

  1989cites this paper
• Enzyme nomenclature. Recommendations 1984. Supplement 2: corrections and additions.

  1989cites this paper
• Determination of protein expression and plasmid copy number from cloned genes in Escherichia coli by flow injection analysis using an enzyme indicator vector

  1989cites this paper
• Identification and characterization of a cyanate permease in Escherichia coli K-12

  1989cites this paper
• Cyanide oxygenase and cyanase activities of Pseudomonas fluorescens NCIMB 11764

  1989cites this paper
• Studies on metabolic pathways of cyanate in rats.

  1988cites this paper
• Characterization of the cyn operon in Escherichia coli K12.

  1988cites this paper
• Reversible dissociation of active octamer of cyanase to inactive dimer promoted by alteration of the sulfhydryl group.

  1988cites this paper
• Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12

  1987cites this paper
• Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase

  1987influential citation
• Bicarbonate is a recycling substrate for cyanase.

  1987cites this paper
• Preliminary X-ray crystallographic study of cyanase from Escherichia coli.

  1987cites this paper
• Interaction of mono- and dianions with cyanase: evidence for apparent half-site binding.

  1987cites this paper
• Isolation and characterization of Escherichia coli mutants lacking inducible cyanase.

  1987cites this paper
• Structural properties of cyanase. Denaturation, renaturation, and role of sulfhydryls and oligomeric structure in catalytic activity.

  1987cites this paper
• Kinetic properties of cyanase.

  1986cites this paper
• Molar absorptivity and A1cm1% values for proteins at selected wavelengths of the visible and ultraviolet regions. XXIV

  1984cites this paper
• The amino acid sequence of Escherichia coli cyanase.

  1983cites this paper
• The conversion of cyanide to ammonia by extracts of a strain of Pseudomonas fluorescens that utilizes cyanide as a source of nitrogen for growth

  1983cites this paper
• Enzyme Nomenclature. Recommendations 1978. Supplement 4: corrections and additions.

  1983cites this paper