Aberrant Methylation of an Imprinted Gene U2af1-rs1(SP2) Caused by Its Own Transgene*

Genomic imprinting refers to the parental allele-specific expression of genes. The precise mechanism underlying this phenomenon, which may involve DNA methylation, is not yet known. U2af1-rs1(SP2) is an imprinted gene expressed from the paternal allele and is methylated on the maternal allele. Here we report an artificial system in which expression and methylation of the endogenous imprinted gene U2af1-rs1 can be affected by interaction with its own transgene in the testis. We suggest that there is a mechanism in male gametogenesis by which the U2af1-rs1 gene is kept unmethylated to be expressed in the offspring in addition to a mechanism in female gametogenesis by which the U2af1-rs1 gene is methylated and is not expressed in the offspring.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-04-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.272.14.9120PMID 9083040
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene

  1995cited by this paper
• Cloning and mapping of the U2af1-rs2 gene with a high transmission distortion in interspecific backcross progeny.

  1995cited by this paper
• DNA methylation and genomic imprinting.

  1994cited by this paper
• Imprinting: a gamete's point of view.

  1994cited by this paper
• New insights reveal complex mechanisms involved in genomic imprinting.

  1994cited by this paper
• Parental imprinting of autosomal mammalian genes.

  1994cited by this paper
• Impressions of imprints.

  1994cited by this paper
• Position-independent, high-level, and correct regional expression of the rat aldolase C gene in the central nervous system of transgenic mice.

  1994cited by this paper
• A new imprinted gene cloned by a methylation-sensitive genome scanning method.

  1993cited by this paper
• A genomic scanning method for higher organisms using restriction sites as landmarks.

  1991cited by this paper
• Differential imprinting and expression of maternal and paternal genomes.

  1988cited by this paper
• The molecular basis of the sparse fur mouse mutation.

  1987cited by this paper
• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

  1987cited by this paper

• Novel insights into gene silencing mechanisms in Zea mays and Arabidopsis thaliana

  2016cites this paper
• Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications

  2016cites this paper
• Cis-acting determinants of paramutation.

  2015cites this paper
• Phenotypic outcomes of imprinted gene models in mice: elucidation of pre- and postnatal functions of imprinted genes.

  2014cites this paper
• Genomic imprinting: A general overview

  2013cites this paper
• Molecular Genetics of Genomic Imprinting

  2011cites this paper
• Characterization of a novel fluorescent reporter of genomic imprinting in the mouse

  2010cites this paper
• Characterization of the murine Angelman syndrome imprinting center

  2010cites this paper
• Characterization of an ectopic beta-globin LCR: Good neighbors and a distant friend

  2009cites this paper
• Antisense transcription occurs at the promoter of a mouse imprinted gene, commd1, on the repressed paternal allele.

  2009cites this paper
• Comparative Genomic Approach Toward Species-Specific Imprinting

  2009cites this paper
• Paramutation: Heritable in trans effects

  2009cites this paper
• Epigenetics, an emerging discipline with broad implications.

  2008cites this paper
• Transvection mediated by the translocated cyclin D1 locus in mantle cell lymphoma

  2008cites this paper
• UvA-DARE ( Digital Academic Repository ) Chromatin looping and epigenetic regulation at the maize b 1 locus

  2008cites this paper
• UvA-DARE ( Digital Academic Repository ) Chromatin looping and epigenetic regulation at the maize b 1 locus

  2008cites this paper
• Molecular biology and genetics / Biologie et génétique moléculaires Epigenetics, an emerging discipline with broad implications

  2008cites this paper
• Paramutation-like effects at the mouse scapinin (Phactr3) locus.

  2008cites this paper
• A Screen for Retrotransposed Imprinted Genes Reveals an Association between X Chromosome Homology and Maternal Germ-Line Methylation

  2006cites this paper
• Genomic imprinting in Dicer1-hypomorphic mice

  2006influential citation
• Genomic Imprinting, Molecular Genetics of

  2006cites this paper
• Molecular Characterization of the mop2, a Gene Required for Epigenetic Silencing

  2006cites this paper
• Paramutation: an encounter leaving a lasting impression.

  2005cites this paper
• Maternal disruption of Ube3a leads to increased expression of Ube3a-ATS in trans

  2005cites this paper
• Chromatin conversations: mechanisms and implications of paramutation

  2004cites this paper
• Genomic imprinting and its effects on genes and chromosomes in mammals.

  2004cites this paper
• Genomic imprinting: could the chromatin structure be the driving force?

  2003cites this paper
• Disruption of the genomic imprint in trans with homologous recombination at Snrpn in ES cells

  2003cites this paper
• Trans allele methylation and paramutation-like effects in mice

  2003cites this paper
• Allele-specific expression analysis by RNA-FISH demonstrates preferential maternal expression of UBE3A and imprint maintenance within 15q11- q13 duplications.

  2002cites this paper
• Anx7 Is Required for Nutritional Control of Gene Expression in Mouse Pancreatic Islets of Langerhans

  2002cites this paper
• An X‐linked GFP transgene reveals unexpected paternal X‐chromosome activity in trophoblastic giant cells of the mouse placenta

  2001cites this paper
• DNA Methylation Is Linked to Deacetylation of Histone H3, but Not H4, on the Imprinted Genes Snrpnand U2af1-rs1

  2001cites this paper
• The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation.

  2000cites this paper
• Genomic imprinting and Beckwith-Wiedemann syndrome.

  2000cites this paper
• Allelic trans-sensing and imprinting.

  1999cites this paper
• Genomic imprinting in mammals: an interplay between chromatin and DNA methylation?

  1999cites this paper
• Cytosine methylation and the unequal developmental potentials of the oocyte and sperm genomes.

  1998cites this paper
• Imprinting mechanisms in mammals.

  1998cites this paper
• Chromatin complexes as aperiodic microcrystalline arrays that regulate genome organisation and expression.

  1998cites this paper
• Gametogenesis '98: Cytosine Methylation and the Unequal Developmental Potential of the Oocyte and Sperm Genomes

  1998cites this paper
• Cosuppression comes to the animals.

  1997cites this paper
• Transactivation of Igf2 in a mouse model of Beckwith–Wiedemann syndrome

  1997cites this paper
• Loss of the maternal H19 gene induces changes in Igf2 methylation in both cis and trans.

  1997cites this paper