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Isolation, characterization and in vitro anticancer activity of an aqueous galactomannan from the seed of Sesbania cannabina.

Mengyi Zhou,Lei Yang,Shilong Yang,Fengyi Zhao,Li Xu,Q. Yong

Published 2018 in International Journal of Biological Macromolecules

ABSTRACT

As a pioneer plant, Sesbania cannabina is cultivated on a large scale for saline-alkali land improvement in China. Here, a native galactomannan (GalM) and a series of its hydrolysates (GalM40, GalM50 and GalM65) were obtained from S. cannabina seed by water extraction, β-mannanase hydrolysate and ethanol precipitation. As elucidated by HPAEC, NMR, FT-IR and HPGPC, GalM was characterized as a β-1,4-linked d-mannose polymer with single α-1,6-linked d-galactose side chain in a 2.4:1M ratio, and had a molecular weight of 1.42×106Da. MTT assay showed that these four fractions had significant inhibitory effect on A549, Hela, HepG2 and MCF-7 in a dose-dependent manner, and that GalM40 with second-highest MW (1.47×104Da) possessed higher activity amongst those fractions. Such strong inhibition effect on the growth of human cancer cells might derive from its ability to increase caspase-12 expression, as revealed from immunohistochemical analysis. In sum, this paper characterizes the molecular structure of S. cannabina galactomannan with anticancer activity, and this new knowledge will provide a basis for its further structure-properties research and ultimately, develop new possibilities for its use in high-value applications, such as functional food.

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