A study of the literature reveals the existence of comparatively few communications related to this particular field. The earliest work appears to be that of Macallum (I), dealing with the inorganic constituents of the blood of vertebrates and invertebrates. Other investigations somewhat closely related were made by Lipschiitz (a), on the metabolism of fishes during starvation; Botazzi (3), on the peritoneal fluid and blood of sea animals; Jona (4), on freezing point values of the blood and body fluids of certain mammals, fish, and crustacea; Okuda (5), dealing with the quantitative determination of creatinine and creatine in some fishes, mollusks, and crustacea; and Fandard and Rant (6), on sugar in the blood of the sea turtle. The results embodied in t,hese communications are greatly limited, however. The earlier workers were handicapped by the lack of accurate methods, and hence could not extend their studies to any great length. In later years the only work which has a comparative value when studied with that of the present paper is that of Denis (7), and of Wilson and Adolph (8), dealing with the determination of certain nitrogenous substances in the blood of fresh and salt water fishes; e.g., shark, ray, mackerel, carp, and a few others. In view of the general lack of information concerning the composition of the blood of invertebrate animals, it is evident that any substantial addition to our knowledge in this field
I. A CHEMICAL STUDY OF THE BLOOD OF SEVERAL INVERTEBRATE ANIMALS
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