Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging.

A laboratory system for exposing aerosol particles to ozone and rapidly measuring the subsequent changes in their single-particle fluorescence is reported. The system consists of a rotating drum chamber and a single-particle fluorescence spectrometer (SPFS) utilizing excitation at 263 nm. Measurements made with this system show preliminary results on the ultra-violet laser-induced-fluorescence (UV-LIF) spectra of single aerosolized particles of Yersinia rohdei, and of MS2 (bacteriophage) exposed to ozone. When bioparticles are exposed in the chamber the fluorescence emission peak around 330 nm: i) decreases in intensity relative to that of the 400-550 nm band; and ii) shifts slightly toward shorter-wavelengths (consistent with further drying of the particles). In these experiments, changes were observed at exposures below the US Environmental Protection Agency (EPA) limits for ozone.

• Publication year

  2012

• Venue

  Optics Express

• Publication date

  2012-12-31

• Fields of study

  Medicine, Materials Science, Chemistry, Environmental Science

• Identifiers
  DOI 10.1364/OE.20.029867PMID 23388813
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Primary biological aerosol particles in the atmosphere: a review

  2012cited by this paper
• Sources of Bacteria in Outdoor Air across Cities in the Midwestern United States

  2011cited by this paper
• Fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric processing

  2011cited by this paper
• Gas uptake and chemical aging of semisolid organic aerosol particles

  2011cited by this paper
• Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements.

  2011cited by this paper
• Improved Method for the Evaluation of Real-Time Biological Aerosol Detection Technologies

  2011cited by this paper
• Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences

  2011cited by this paper
• Aerosol survival of Staphylococcus epidermidis.

  2011cited by this paper
• Fluorescence spectra of atmospheric aerosol particles measured using one or two excitation wavelengths: comparison of classification schemes employing different emission and scattering results.

  2010cited by this paper
• Development of a novel real-time pollen-sorting counter using species-specific pollen autofluorescence

  2010cited by this paper
• Measurements and comparison of primary biological aerosol above and below a tropical forest canopy using a dual channel fluorescence spectrometer

  2010cited by this paper
• Bioaerosol analysis with Raman chemical imaging microspectroscopy.

  2009cited by this paper
• Characterization of Airborne Microbial Communities at a High-Elevation Site and Their Potential To Act as Atmospheric Ice Nuclei

  2009cited by this paper
• Optical Techniques for Detecting and Identifying Biological-Warfare Agents

  2009cited by this paper
• Fluorescence cross sections of bioaerosols and suspended biological agents.

  2009cited by this paper
• Particle-fluorescence spectrometer for real-time single-particle measurements of atmospheric organic carbon and biological aerosol.

  2009cited by this paper
• High diversity of fungi in air particulate matter

  2009cited by this paper
• LIF bio-aerosol threat triggers: then and now

  2009cited by this paper
• Fluorescent biological aerosol particle concentrations and size distributions measured with an Ultraviolet Aerodynamic Particle Sizer (UV-APS) in Central Europe

  2009cited by this paper
• Reduction by gaseous ozone of Salmonella and microbial flora associated with fresh-cut cantaloupe.

  2008cited by this paper
• The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation

  2008cited by this paper
• Single‐particle laser‐induced‐fluorescence spectra of biological and other organic‐carbon aerosols in the atmosphere: Measurements at New Haven, Connecticut, and Las Cruces, New Mexico

  2007cited by this paper
• Effect of ozone and open air factor against aerosolized Micrococcus luteus.

  2007cited by this paper
• Performance assessment of UVAPS: Influence of fungal spore age and air exposure

  2007cited by this paper
• Aging of organic aerosol: bridging the gap between laboratory and field studies.

  2007cited by this paper
• under a Creative Commons License. Atmospheric Chemistry

  2007cited by this paper
• Atmospheric organic and bio-aerosols as cloud condensation nuclei (CCN): A review

  2006cited by this paper
• Single particle multichannel bio-aerosol fluorescence sensor.

  2005cited by this paper
• Interhemispheric transport of viable fungi and bacteria from Africa to the Caribbean with soil dust

  2005cited by this paper
• Diurnal variations in the hygroscopic growth cycles of ambient aerosol populations

  2005cited by this paper
• Multiple UV wavelength excitation and fluorescence of bioaerosols

  2004cited by this paper
• Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland, USA: measurement and classification of single particles containing organic carbon

  2004cited by this paper
• Pollen Chemosensitivity to Ozone and Peroxides

  2004cited by this paper
• Autofluorescence of Plant Secreting Cells as a Biosensor and Bioindicator Reaction

  2003cited by this paper
• Performance evaluation of the UVAPS: influence of physiological age of airborne bacteria and bacterial stress

  2003cited by this paper
• Aerosol Characteristics in a Subway Environment

  2003cited by this paper
• Single-Particle Fluorescence Spectrometer for Ambient Aerosols

  2003cited by this paper
• Interaction of ozone and negative air ions to control micro‐organisms

  2002cited by this paper
• Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity.

  2001cited by this paper
• Dynamics of photon-induced degradation and fluorescence in riboflavin microparticles

  2001cited by this paper
• Inactivation Kinetics of Foodborne Spoilage and Pathogenic Bacteria by Ozone

  2000cited by this paper
• Electrospray mass and tandem mass spectrometry identification of ozone oxidation products of amino acids and small peptides

  2000cited by this paper
• Bio-aerosol fluorescence sensor

  1999cited by this paper
• Size and Fluorescence Measurements for Field Detection of Biological Aerosols

  1999cited by this paper
• Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence.

  1997cited by this paper
• Reaction of ozone with protein tryptophans: band III, serum albumin, and cytochrome C.

  1997cited by this paper
• Comparison of the Effects of Ozone on the Modification of Amino Acid Residues in Glutamine Synthetase and Bovine Serum Albumin (*)

  1996cited by this paper
• Aerosol-fluorescence spectrum analyzer: real-time measurement of emission spectra of airborne biological particles.

  1995cited by this paper
• The cascade mechanism to explain ozone toxicity: the role of lipid ozonation products.

  1995cited by this paper
• A kinetic model for the competitive reactions of ozone with amino acid residues in proteins in reverse micelles.

  1993cited by this paper
• How far does ozone penetrate into the pulmonary air/tissue boundary before it reacts?

  1992cited by this paper
• Use of the method of tryptophan fluorescence to characterize disruptions of the structure of ozonized proteins

  1988cited by this paper
• The optimum rate of drum rotation for aerosol aging

  1987cited by this paper
• Changes induced by ozone and ultraviolet light in type I collagen. Bovine Achilles tendon collagen versus rat tail tendon collagen.

  1985cited by this paper
• Spectral-fluorescent investigation of the action of ozone on aromatic amino acids

  1982cited by this paper
• Reaction of ozone with nicotinamide and its derivatives.

  1974cited by this paper
• Comparative toxicity of various ozonized olefins to bacteria suspended in air

  1970cited by this paper
• Reaction of ozone with amino acids and proteins.

  1969cited by this paper
• Effect of Aerosol Age on the Infectivity of Airborne Pasteurella tularensis for Macaca mulatta and Man

  1966cited by this paper
• The use of a rotating drum for the study of aerosols over extended periods of time.

  1958cited by this paper
• Atmospheric Chemistry and Physics the Fluorescence Properties of Aerosol Larger than 0.8 Μm in Urban and Tropical Rainforest Locations

  year unknowncited by this paper

• Meteorological and Air Quality Effects on Bioaerosol Detection Using WIBS-NEO and IBAC-2 in Dublin City

  2026cites this paper
• The Impact of Environmental Aging Processing on Bioaerosol Detection Using Circular Intensity Differential Scattering (CIDS)

  2025cites this paper
• Alteration of the health effects of bioaerosols by chemical modification in the atmosphere: A review

  2023cites this paper
• Environmental sampling for disease surveillance: Recent advances and recommendations for best practice

  2023cites this paper
• Advances in Spectral Techniques for Detection of Pathogenic Microorganisms

  2022cites this paper
• Captive Aerosol Growth and Evolution (CAGE) chamber system to investigate particle growth due to secondary aerosol formation

  2021cites this paper
• Atmospheric aging processes of bioaerosols under laboratory-controlled conditions: A review

  2021cites this paper
• Bioaerosol property and viability affected by various Environmental factors

  2020cites this paper
• Bioaerosol field measurements: Challenges and perspectives in outdoor studies

  2020cites this paper
• Natural sources and experimental generation of bioaerosols: Challenges and perspectives

  2020cites this paper
• Laboratory study of bioaerosols: Traditional test systems, modern approaches, and environmental control

  2020cites this paper
• Changes of fluorescence spectra and viability from aging aerosolized E. coli cells under various laboratory-controlled conditions in an advanced rotating drum

  2019influential citation
• Experimental Characterization of Aerosol Suspension in a Rotating Drum

  2019cites this paper
• Experimental Characterization of Aerosol Suspension in a Rotating 1 Drum 2 3

  2018cites this paper
• The interaction of pollution, meteorology and bioaerosols : implications on human health

  2018cites this paper
• Online Techniques for Quantification and Characterization of Biological Aerosols

  2017cites this paper
• Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

  2017cites this paper
• Aerobiology: Experimental Considerations, Observations, and Future Tools

  2017cites this paper
• Characterizing Responses of Primary Biological Aerosols to Oxidative Atmospheric Conditions

  2016cites this paper
• Bioaerosols in the Earth system: Climate, health, and ecosystem interactions

  2016cites this paper
• Atmospheric chemistry of bioaerosols: heterogeneous and multiphase reactions with atmospheric oxidants and other trace gases

  2016cites this paper
• Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

  2015cites this paper
• Fluorescence spectra and biological activity of aerosolized bacillus spores and MS2 bacteriophage exposed to ozone at different relative humidities in a rotating drum

  2015cites this paper
• Ground‐based measurement of fluorescent aerosol particles in Tokyo in the spring of 2013: Potential impacts of nonbiological materials on autofluorescence measurements of airborne particles

  2015cites this paper
• Continuous observation of ion beam induced luminescence spectra from organic standard targets

  2015cites this paper
• Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

  2015cites this paper
• Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory.

  2014cites this paper
• Design of an environmentally controlled rotating chamber for bioaerosol aging studies

  2014cites this paper
• Bioaerosol Detection with Fluorescence Spectroscopy

  2014cites this paper
• Effects of ozone and relative humidity on fluorescence spectra of octapeptide bioaerosol particles

  2014cites this paper
• A laboratory exposure system to study the effects of aging on super-micron aerosol particles

  2014cites this paper
• Characterisation of bioaerosol emissions from a Colorado pine forest : results from the BEACHON-RoMBAS experiment

  2014cites this paper
• Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria.

  2013cites this paper
• Variation in the fluorescence intensity of thermally-exposed bacterial bioaerosols

  2013cites this paper