Prevalence of Lyme borrelia in Ixodes persulcatus ticks from an area with a confirmed case of Lyme disease.

In this study, the prevalence of Borrelia infections in Ixodes ticks from a site in Hokkaido, Japan, with confirmed cases of Lyme disease was determined by a PCR method capable of detecting and differentiating between strains of pathogenic Borrelia, with particular emphasis on Borrelia garinii (B. garinii) and Borrelia afzelli (B. afzelli), using tick-derived DNA extracts as template. A total of 338 ticks, inclusive of 284 Ixodes persulcatus (I. persulcatus), were collected by flagging vegetation in mid-spring. Ninety-eight (34.5%) of I. persulcatus tested positive for Borrelia species DNA, whereas the overall prevalence of Borrelia species in Ixodes ovatus and Haemaphysalis longicornis ticks was 19.5 and 7.7%, respectively. PCR-RFLP and sequence analysis of Borrelia rrf(5S)-rrl(23S) intergenic spacer DNA amplicons indicated that they originated from three different Borrelia species namely, B. garinii, B. afzelii and B. japonica. Among the I. persulcatus species, which is a known vector of human borreliosis, 86 were mono-infected with B. garinii, 2 ticks were mono-infected with B. afzelii and whereas 12 ticks had dual infections. Most significant, 11 of the I. persulcatus ticks were coinfected with Anaplasma phagocytophilum and B. garinii. The difference between the number of obtained and expected co-infections was significant (χ(2)=4.32, P=0.038).

• Publication year

  2013

• Venue

  Journal of Veterinary Medical Science

• Publication date

  2013-02-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1292/JVMS.12-0211PMID 23047332
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Serological Survey of Five Zoonoses, Scrub Typhus, Japanese Spotted Fever, Tularemia, Lyme Disease, and Q Fever, in Feral Raccoons (Procyon lotor) in Japan

  2011cited by this paper
• Lyme disease: Part I. Advances and perspectives.

  2011cited by this paper
• Molecular detection of Anaplasma phagocytophilum in cattle and Ixodes persulcatus ticks.

  2011cited by this paper
• Establishment of a laboratory colony of taiga tick Ixodes persulcatus for tick-borne pathogen transmission studies.

  2008cited by this paper
• First Molecular Detection of Borrelia afzelii in Clinical Samples in Korea

  2007cited by this paper
• Attachment Duration Required for Rhipicephalus appendiculatus to Transmit Theileria parva to the Host

  2007cited by this paper
• Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection Enhances Chemokine, Cytokine, and Matrix Metalloprotease Expression by Human Brain Microvascular Endothelial Cells

  2007cited by this paper
• Coinfections Acquired from Ixodes Ticks

  2006cited by this paper
• Coinfection with Anaplasma phagocytophilum Alters Borrelia burgdorferi Population Distribution in C3H/HeN Mice

  2005cited by this paper
• Anaplasma phagocytophilum in White-tailed Deer

  2005cited by this paper
• The emergence of Lyme disease.

  2004cited by this paper
• Terrestrial distribution of the Lyme borreliosis agent Borrelia burgdorferi sensu lato in East Asia.

  2004cited by this paper
• Prevalence of Anaplasma phagocytophila and Borrelia burgdorferi in Ixodes persulcatus ticks from northeastern China.

  2003cited by this paper
• Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

  2003cited by this paper
• Mitochondrial Sequence Variation in Carios capensis (Neumann), a Parasite of Seabirds, Collected on Torishima Island in Japan

  2003cited by this paper
• Genetic Variants of Ehrlichia phagocytophila, Rhode Island and Connecticut

  2002cited by this paper
• Disease-specific diagnosis of coinfecting tickborne zoonoses: babesiosis, human granulocytic ehrlichiosis, and Lyme disease.

  2002cited by this paper
• Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in Northern Colorado.

  2000cited by this paper
• Simultaneous human granulocytic ehrlichiosis and Lyme borreliosis.

  1997cited by this paper
• Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

  1996cited by this paper
• Mixed infection of different Borrelia species among Apodemus speciosus mice in Hokkaido, Japan

  1995cited by this paper
• Susceptibility of Ixodes persulcatus and I. ovatus (Acari: Ixodidae) to Lyme disease spirochetes isolated from humans in Japan.

  1994cited by this paper
• Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons.

  1994cited by this paper
• Characterization of Borrelia burgdorferi isolated from Ixodes persulcatus and Ixodes ovatus ticks in Japan.

  1992cited by this paper
• Prevalence of Lyme borreliosis spirochetes in ixodid ticks of Japan, with special reference to a new potential vector, Ixodes ovatus (Acari: Ixodidae).

  1992cited by this paper
• Detection of Borrelia burgdorferi in ixodid ticks collected from the Chichibu mountainous region of Central Honshu, Japan

  1992cited by this paper
• Lyme disease in Japan and its possible incriminated tick vector, Ixodes persulcatus.

  1987influential reference

• Differences in Tick Infestation Intensity by Season, Sex, Age Class, and Body Region of Feral Raccoons (Procyon lotor) in the Miura Peninsula, Japan

  2024cites this paper
• Prevalence of three important tick-borne pathogens in ticks and humans in Shaanxi Province, Northwest China.

  2024cites this paper
• Molecular detection of Borrelia burgdorferi (sensu lato) and Rickettsia spp. in hard ticks distributed in Tokachi District, eastern Hokkaido, Japan

  2021influential citation
• Reconstruction of mitochondrial genomes from raw sequencing data provides insights on the phylogeny of Ixodes ticks and cautions for species misidentification.

  2021cites this paper
• Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group

  2021cites this paper
• Large erythema migrans lesion in Lyme disease

  2020cites this paper
• Study on the Epidemiological Features of Lyme Disease in Korea between 2011 and 2018

  2019cites this paper
• Borreliella

  2019cites this paper
• TRENDS IN EPIDEMIOLOGICAL ASPECTS OF LYME DISEASE WITH DOMESTIC AND EXOTIC INFECTIONS IN REPUBLIC OF KOREA, 2011-2017

  2019cites this paper
• Prevalence and Identification of Borrelia burgdorferi Sensu Lato Genospecies in Ticks from Northeastern China

  2019influential citation
• Failure of the Asian longhorned tick, Haemaphysalis longicornis, to serve as an experimental vector of the Lyme disease spirochete, Borrelia burgdorferi sensu stricto

  2019cites this paper
• Clinical Evidence of Lyme Disease in Dogs and Disease Awareness Among Students and Veterinarians in Veracruz, Mexico

  2019cites this paper
• A case of Lyme disease in a Japanese woman

  2018cites this paper
• The role of sika deer in the transmission of Borrelia spp. in Hokkaido, Japan 北海道における Borrelia spp. の伝播に対する 鹿の役割

  2018cites this paper
• Correlation between chronic arthritis patients confirmed with questionnaire and serologic test of Lyme disease

  2018cites this paper
• Multilocus sequence analysis of Borrelia burgdorferi sensu lato isolates from Western Siberia, Russia and Northern Mongolia.

  2018cites this paper
• Is there a Lyme-like disease in Australia? Summary of the findings to date

  2016influential citation
• Host-Vector-Pathogen Interactions of Tick-Borne Diseases in Hokkaido Wildlife

  2016cites this paper
• Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze.

  2016cites this paper
• Perfil inibitório e antigenicidade da cistatina JpIocys2a de Ixodes ovatus

  2015cites this paper
• Serological survey of Borrelia infection of dogs in Sapporo, Japan, where Borrelia garinii infection was previously detected

  2015cites this paper
• Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response

  2015cites this paper
• Epidemiological features and clinical manifestations of Lyme borreliosis in Korea during the period 2005-2012.

  2015cites this paper
• Prevalence of Borrelia burgdorferi in Adult Female Ticks (Ixodes scapularis), Wisconsin 2010–2013

  2015cites this paper
• An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes.

  2015cites this paper
• RUNNING HEAD: SEROLOGICAL SURVEY OF BORRELIA IN DOGS

  2015cites this paper
• A relapsing fever group Borrelia sp. similar to Borrelia lonestari found among wild sika deer (Cervus nippon yesoensis) and Haemaphysalis spp. ticks in Hokkaido, Japan.

  2014cites this paper