The bZIP Transcription Factor Fgap1 Mediates Oxidative Stress Response and Trichothecene Biosynthesis But Not Virulence in Fusarium graminearum

Redox sensing is of primary importance for fungi to cope with oxidant compounds found in their environment. Plant pathogens are particularly subject to the oxidative burst during the primary steps of infection. In the budding yeast Saccharomyces cerevisiae, it is the transcription factor Yap1 that mediates the response to oxidative stress via activation of genes coding for detoxification enzymes. In the cereal pathogen Fusarium graminearum, Fgap1 a homologue of Yap1 was identified and its role was investigated. During infection, this pathogen produces mycotoxins belonging to the trichothecenes family that accumulate in the grains. The global regulation of toxin biosynthesis is not completely understood. However, it is now clearly established that an oxidative stress activates the production of toxins by F. graminearum. The involvement of Fgap1 in this activation was investigated. A deleted mutant and a strain expressing a truncated constitutive form of Fgap1 were constructed. None of the mutants was affected in pathogenicity. The deleted mutant showed higher level of trichothecenes production associated with overexpression of Tri genes. Moreover activation of toxin accumulation in response to oxidative stress was no longer observed. Regarding the mutant with the truncated constitutive form of Fgap1, toxin production was strongly reduced. Expression of oxidative stress response genes was not activated in the deleted mutant and expression of the gene encoding the mitochondrial superoxide dismutase MnSOD1 was up-regulated in the mutant with the truncated constitutive form of Fgap1. Our results demonstrate that Fgap1 plays a key role in the link between oxidative stress response and F. graminearum secondary metabolism.

• Publication year

  2013

• Venue

  PLoS ONE

• Publication date

  2013-12-12

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0083377PMID 24349499PMCID 3861502
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Coupling of transcriptional response to oxidative stress and secondary metabolism regulation in filamentous fungi

  2015cited by this paper
• The ATF/CREB transcription factor Atf1 is essential for full virulence, deoxynivalenol production, and stress tolerance in the cereal pathogen Fusarium graminearum.

  2013cited by this paper
• On the trail of a cereal killer: recent advances in Fusarium graminearum pathogenomics and host resistance.

  2012cited by this paper
• Aoyap1 regulates OTA synthesis by controlling cell redox balance in Aspergillus ochraceus

  2012influential reference
• ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 49

  2011cited by this paper
• The bZIP Transcription Factor MoAP1 Mediates the Oxidative Stress Response and Is Critical for Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae

  2011influential reference
• Trichothecenes: From Simple to Complex Mycotoxins

  2011influential reference
• Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease.

  2011cited by this paper
• Response of germinating barley seeds to Fusarium graminearum: The first molecular insight into Fusarium seedling blight.

  2011cited by this paper
• Mitochondrial quality control in aging and lifespan control of the fungal aging model Podospora anserina.

  2011cited by this paper
• Regulation of trichothecene biosynthesis in Fusarium: recent advances and new insights

  2011cited by this paper
• Truncated Afyap1 Attenuates Antifungal Susceptibility of Aspergillus fumigatus to Voriconazole and Confers Adaptation of the Fungus to Oxidative Stress

  2010cited by this paper
• The Yap family and its role in stress response

  2010cited by this paper
• High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains.

  2010cited by this paper
• Nutrient profiling reveals potent inducers of trichothecene biosynthesis in Fusarium graminearum.

  2009cited by this paper
• Fusarium response to oxidative stress by H2O2 is trichothecene chemotype-dependent.

  2009cited by this paper
• Nonsense and Missense Mutations in FCY2 and FCY1 Genes Are Responsible for Flucytosine Resistance and Flucytosine-Fluconazole Cross-Resistance in Clinical Isolates of Candida lusitaniae

  2009cited by this paper
• Does botrytis cinerea Ignore H(2)O(2)-induced oxidative stress during infection? Characterization of botrytis activator protein 1.

  2009influential reference
• Developing kernel and rachis node induce the trichothecene pathway of Fusarium graminearum during wheat head infection.

  2009cited by this paper
• Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium

  2009cited by this paper
• Coordinate control of oxidative stress tolerance, vegetative growth, and fungal pathogenicity via the AP1 pathway in the rough lemon pathotype of Alternaria alternata.

  2009cited by this paper
• Global gene regulation by Fusarium transcription factors Tri6 and Tri10 reveals adaptations for toxin biosynthesis

  2009cited by this paper
• Roles of reactive oxygen species in interactions between plants and pathogens

  2008cited by this paper
• Modulation of Antioxidant Defense in Aspergillus parasiticus Is Involved in Aflatoxin Biosynthesis: a Role for the ApyapA Gene

  2008cited by this paper
• Exogenous H2O2 and catalase treatments interfere with Tri genes expression in liquid cultures of Fusarium graminearum

  2007cited by this paper
• Molecular and Genetic Studies of Fusarium Trichothecene Biosynthesis: Pathways, Genes, and Evolution

  2007cited by this paper
• Oxidative stress in Schizosaccharomyces pombe: different H2O2 levels, different response pathways

  2006cited by this paper
• Accumulation of deoxynivalenol and its 15-acetylated form is significantly modulated by oxidative stress in liquid cultures of Fusarium graminearum.

  2006cited by this paper
• The Cryptococcus neoformans Catalase Gene Family and Its Role in Antioxidant Defense

  2006cited by this paper
• Overproduction of a single protein, Pc-Pex11p, results in 2-fold enhanced penicillin production by Penicillium chrysogenum.

  2005cited by this paper
• Effects of trichothecene mycotoxins on eukaryotic cells: A review

  2005cited by this paper
• Fundamental contribution of beta-oxidation to polyketide mycotoxin production in planta.

  2005cited by this paper
• Identification of proteins induced or upregulated by Fusarium head blight infection in the spikes of hexaploid wheat (Triticum aestivum).

  2005cited by this paper
• Activation of an AP1-Like Transcription Factor of the Maize Pathogen Cochliobolus heterostrophus in Response to Oxidative Stress and Plant Signals

  2005cited by this paper
• Fusarium head blight: epidemiological origin of the effects of cultural practices on head blight attacks and the production of mycotoxins by Fusarium in wheat grains

  2004cited by this paper
• Heading for disaster: Fusarium graminearum on cereal crops.

  2004cited by this paper
• The Gpmk1 MAP kinase of Fusarium graminearum regulates the induction of specific secreted enzymes

  2004cited by this paper
• Yeast activator proteins and stress response: an overview

  2004cited by this paper
• Multiple Sequence Alignment Using ClustalW and ClustalX

  2003cited by this paper
• Regulation of the Transcriptional Response to Oxidative Stress in Fungi: Similarities and Differences

  2003cited by this paper
• Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

  2002cited by this paper
• The Molecular Biology Database Collection: 2002 update

  2002cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001influential reference
• The bZip Transcription Factor Cap1p Is Involved in Multidrug Resistance and Oxidative Stress Response inCandida albicans

  1999cited by this paper
• Yap1 and Skn7 Control Two Specialized Oxidative Stress Response Regulons in Yeast*

  1999cited by this paper
• Crm1p mediates regulated nuclear export of a yeast AP‐1‐like transcription factor

  1998cited by this paper
• Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.

  1998cited by this paper
• Oxidative stress responses of the yeast Saccharomyces cerevisiae

  1998cited by this paper
• Regulation of yAP‐1 nuclear localization in response to oxidative stress

  1997cited by this paper
• Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene.

  1995cited by this paper
• bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamily

  1995cited by this paper
• Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance.

  1993cited by this paper
• Isolation of telomeric DNA from the filamentous fungus Podospora anserina and construction of a self-replicating linear plasmid showing high transformation frequency.

  1993cited by this paper
• Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase.

  1990cited by this paper
• Basic local alignment search tool.

  1990influential reference
• Molecular transformation of Fusarium solani with an antibiotic resistance marker having no fungal DNA homology

  1989cited by this paper
• Macroconidium Formation in Submerged Cultures by a Nonsporulating Strain of Gibberella Zeae

  1965cited by this paper

• MrBZIP, a MrWETA-interacting transcription factor, mediates oxidative and heat stress tolerance in conidia of Metarhizium robertsii.

  2026cites this paper
• Role of enniatins and deoxynivalenol in interspecific growth and competition in vitro among the principal causal agents of Fusarium head blight

  2026cites this paper
• MeJA inhibits fungal growth and DON toxin production by interfering with the cAMP-PKA signaling pathway in the wheat scab fungus Fusarium graminearum

  2025cites this paper
• Recent advances in the biosynthetic mechanisms, regulation, and detoxification strategies of deoxynivalenol in Fusarium graminearum

  2025cites this paper
• Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11’s Impact on Fusarium TR4 Pathogenicity

  2025cites this paper
• Antioxidant Systems of Plant Pathogenic Fungi: Functions in Oxidative Stress Response and Their Regulatory Mechanisms

  2024cites this paper
• Characterisation of sorghum cultivated in Hungary: potential health risk resulting from the occurrence of toxigenic fungi and effect of endogenous lipophilic antioxidants on mycotoxin production

  2024cites this paper
• The Antifungal and Inhibitory Effects of Massoia Essential Oil and C10 Massoia Lactone on Mycotoxin Production in Fusarium graminearum KACC 41047

  2024cites this paper
• Dual RNA-sequencing of Fusarium head blight resistance in winter wheat

  2024cites this paper
• Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot.

  2023cites this paper
• Global Transcriptomic Changes Elicited by sodB Deletion and Menadione Exposure in Aspergillus nidulans

  2023cites this paper
• PeAP1-mediated oxidative stress response plays an important role in the growth and pathogenicity of Penicillium expansum

  2023cites this paper
• Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem

  2023cites this paper
• Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle

  2023cites this paper
• Role of AtYap1 in the reactive oxygen species regulation of lovastatin production in Aspergillus terreus

  2023cites this paper
• Magnaporthe oryzae Transcription Factor MoBZIP3 Regulates Appressorium Turgor Pressure Formation during Pathogenesis

  2022cites this paper
• Genomic footprints related with adaptation and fumonisins production in Fusarium proliferatum

  2022cites this paper
• Microbe Related Chemical Signalling and Its Application in Agriculture

  2022cites this paper
• Insights into the Underlying Mechanism of Ochratoxin A Production in Aspergillus niger CBS 513.88 Using Different Carbon Sources

  2022cites this paper
• Research on the Molecular Interaction Mechanism between Plants and Pathogenic Fungi

  2022cites this paper
• The aflatoxin inhibitors capsaicin and piperine from Capsicum chinense and Piper nigrum fruits modulate the antioxidant system in Aspergillus parasiticus

  2022cites this paper
• Genome-Wide Identification and Expression Analysis of the Basic Leucine Zipper (bZIP) Transcription Factor Gene Family in Fusarium graminearum

  2022cites this paper
• bZIP transcription factors PcYap1 and PcRsmA link oxidative stress response to secondary metabolism and development in Penicillium chrysogenum

  2022cites this paper
• Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins

  2022cites this paper
• Transcriptome analysis of Magnaporthe oryzae reveals the role of histidine phosphotransferase in pathogenesis and stress adaptation

  2022cites this paper
• Bioprospecting Phenols as Inhibitors of Trichothecene-Producing Fusarium: Sustainable Approaches to the Management of Wheat Pathogens

  2022cites this paper
• Transcriptome analysis and functional validation identify a putative bZIP transcription factor, Fpkapc, that regulates development, stress responses, and virulence in Fusarium pseudograminearum.

  2022cites this paper
• Transcription factor control of virulence in phytopathogenic fungi

  2021cites this paper
• A Putative C2H2 Transcription Factor CgTF6, Controlled by CgTF1, Negatively Regulates Chaetoglobosin A Biosynthesis in Chaetomium globosum

  2021cites this paper
• Biosynthesis, evolution and ecology of microbial terpenoids.

  2021cites this paper
• The bZIP Transcription Factor LtAP1 Modulates Oxidative Stress Tolerance and Virulence in the Peach Gummosis Fungus Lasiodiplodia theobromae

  2021cites this paper
• Wheat–Fusarium graminearum Interactions Under Sitobion avenae Influence: From Nutrients and Hormone Signals

  2021cites this paper
• The NADPH Oxidase A of Verticillium dahliae Is Essential for Pathogenicity, Normal Development, and Stress Tolerance, and It Interacts with Yap1 to Regulate Redox Homeostasis

  2021cites this paper
• Surviving the odds: From perception to survival of fungal phytopathogens under host-generated oxidative burst

  2021cites this paper
• MfOfd1 is crucial for stress responses and virulence in the peach brown rot fungus Monilinia fructicola

  2020cites this paper
• Yap1 homologs mediate more than the redox regulation of the antioxidant response in filamentous fungi.

  2020cites this paper
• Restricting mycotoxins without killing the producers: a new paradigm in nano-fungal interactions

  2020cites this paper
• The bZIP Transcription Factor AflRsmA Regulates Aflatoxin B1 Biosynthesis, Oxidative Stress Response and Sclerotium Formation in Aspergillus flavus

  2020cites this paper
• CaASR1 promotes SA- but represses JA-dependent signaling to enhance resistance of Capsicum annuum to bacterial wilt by modulating CabZIP63.

  2020cites this paper
• Analysis of TabZIP15 transcription factor from Trichoderma asperellum ACCC30536 and its function under pathogenic toxin stress

  2020cites this paper
• Effect of H2A.Z deletion is rescued by compensatory mutations in Fusarium graminearum

  2020cites this paper
• Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by Aspergillus westerdijkiae and Penicillium verrucosum

  2020cites this paper
• Investigating the Efficiency of Hydroxycinnamic Acids to Inhibit the Production of Enniatins by Fusarium avenaceum and Modulate the Expression of Enniatins Biosynthetic Genes

  2020cites this paper
• Use of Ecologically-and Evolutionary Relevant Transcriptomic Data to Infer Functions of Fungal Pathogen Gene Orthologs Important for Limiting Fungal Stresses Caused by Interacting Host Plants and Bacteria

  2020cites this paper
• Oxidant-Sensing Pathways in the Responses of Fungal Pathogens to Chemical Stress Signals

  2019cites this paper
• Insights into VdCmr1-mediated protection against high temperature stress and UV irradiation in Verticillium dahliae.

  2019cites this paper
• Genetic regulation of aflatoxin, ochratoxin A, trichothecene, and fumonisin biosynthesis: A review

  2019cites this paper
• Revisiting the Standard Soil Test: The Potential of Macro- and Microbiological Measures to Improve Agricultural Health and Productivity

  2019cites this paper
• Using mushroom-forming fungi in preventing and reducing mycotoxins in cereal products

  2019cites this paper
• The bZIP transcription factor Afap1 mediates the oxidative stress response and aflatoxin biosynthesis in Aspergillus flavus.

  2019cites this paper
• Fusarium graminearum Trichothecene Mycotoxins: Biosynthesis, Regulation, and Management.

  2019cites this paper
• Activation of Aflatoxin Biosynthesis Alleviates Total ROS in Aspergillus parasiticus

  2018cites this paper
• Novel osmotic stress control strategy for improved pneumocandin B0 production in Glarea lozoyensis combined with a mechanistic analysis at the transcriptome level

  2018cites this paper
• The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum.

  2018cites this paper
• Cooperative interactions between seed-borne bacterial and air-borne fungal pathogens on rice

  2018cites this paper
• The bZIP transcriptional factor activator protein-1 regulates Metarhizium rileyi morphology and mediates microsclerotia formation

  2018cites this paper
• FuNTAP: A Fusarium graminearum Protein-Protein Interaction Network to Study Deoxynivalenol Biosynthesis Pathways

  2018cites this paper
• Reactive oxygen species metabolism and plant-fungal interactions.

  2018cites this paper
• Genome-wide functional characterization of putative peroxidases in the head blight fungus Fusarium graminearum.

  2018influential citation
• The role of transcription factor in wheat defense against pathogen and its prospect in breeding 1

  2018cites this paper
• Mycotoxin Biosynthesis and Central Metabolism Are Two Interlinked Pathways in Fusarium graminearum, as Demonstrated by the Extensive Metabolic Changes Induced by Caffeic Acid Exposure

  2018cites this paper
• Adaptation to the Host Environment by Plant-Pathogenic Fungi.

  2017cites this paper
• YAP1 homologue-mediated redox sensing is crucial for a successful infection by Monilinia fructicola.

  2017influential citation
• Citrate-Coated Silver Nanoparticles Growth-Independently Inhibit Aflatoxin Synthesis in Aspergillus parasiticus.

  2017cites this paper
• Functional characterization of two bZIP transcription factors in Verticillium dahliae.

  2017cites this paper
• Morphological changes and increase of resistance to oxidative stress by overexpression of the LebZIP2 gene in Nicotiana benthamiana

  2016cites this paper
• Is the Fgap1 mediated response to oxidative stress chemotype dependent in Fusarium graminearum?

  2016influential citation
• bZIP transcription factor CgAP1 is essential for oxidative stress tolerance and full virulence of the poplar anthracnose fungus Colletotrichum gloeosporioides.

  2016cites this paper
• Multifaceted impact of trichothecene metabolites on plant-microbe interactions and human health

  2016cites this paper
• Regulation and Role of Fungal Secondary Metabolites.

  2016cites this paper
• Base génétique et potentiel d’évolution de la pathogénicité de Fusarium graminearum, bio-agresseur fongique des céréales

  2016cites this paper
• Pathogenicity and trichothecenes production of Fusarium culmorum strains causing head blight on wheat and evaluation of resistance of the varieties cultivated in Algeria

  2016cites this paper
• Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence

  2016cites this paper
• A cytoplasmic Cu-Zn superoxide dismutase SOD1 contributes to hyphal growth and virulence of Fusarium graminearum.

  2016cites this paper
• Basic Leucine Zipper (bZIP) Domain Transcription Factor MBZ1 Regulates Cell Wall Integrity, Spore Adherence, and Virulence in Metarhizium robertsii *

  2015cites this paper
• Autoregulation of ZEB2 expression for zearalenone production in Fusarium graminearum

  2015cites this paper
• Translating biosynthetic gene clusters into fungal armor and weaponry.

  2015cites this paper
• Real-time imaging of hydrogen peroxide dynamics in vegetative and pathogenic hyphae of Fusarium graminearum

  2015cites this paper
• Plant phenolic compounds and oxidative stress: integrated signals in fungal–plant interactions

  2015cites this paper
• The bZIP transcription factor PfZipA regulates secondary metabolism and oxidative stress response in the plant endophytic fungus Pestalotiopsis fici.

  2015cites this paper
• Unraveling incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics

  2015cites this paper
• Glucosinolate-derived isothiocyanates impact mitochondrial function in fungal cells and elicit an oxidative stress response necessary for growth recovery

  2015cites this paper
• The AreA transcription factor mediates the regulation of deoxynivalenol (DON) synthesis by ammonium and cyclic adenosine monophosphate (cAMP) signalling in Fusarium graminearum.

  2015cites this paper
• Mycoremediation with mycotoxin producers: a critical perspective

  2015cites this paper
• Mycotoxins are a component of Fusarium graminearum stress-response system

  2015cites this paper
• System-wide characterization of bZIP transcription factor proteins involved in infection-related morphogenesis of Magnaporthe oryzae.

  2015cites this paper
• Transcriptome analysis of wheat inoculated with Fusarium graminearum

  2015cites this paper
• FgSKN7 and FgATF1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in Fusarium graminearum.

  2015cites this paper
• ROS and 9-oxylipins are correlated with deoxynivalenol accumulation in the germinating caryopses of Triticum aestivum after Fusarium graminearum infection

  2014cites this paper
• Mécanismes moléculaires impliqués dans la modulation de la production de trichothécènes de type B par Fusarium graminearum en réponse au stress oxydant

  2013cites this paper