The F-actin bundler α-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast

The highly dynamic bundling activity of fission yeast α-actinin SpAin1 was biochemically characterized, and its importance for contractile ring formation in vivo was tested. Investigation of a mutant with higher bundling activity, SpAin1(216E), revealed that dynamic SpAin1-mediated bundling is crucial for proper ring assembly and constriction.

• Publication year

  2016

• Venue

  Molecular Biology of the Cell

• Publication date

  2016-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E16-01-0010PMID 27075176PMCID 4884072
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Characterisation of Schizosaccharomyces pombe α-actinin

  2016influential reference
• Mechanistic insights into the anchorage of the contractile ring by anillin and Mid1.

  2015cited by this paper
• Dynamic network morphology and tension buildup in a 3D model of cytokinetic ring assembly.

  2014cited by this paper
• Mechanism of cytokinetic contractile ring constriction in fission yeast.

  2014influential reference
• Ena/VASP Enabled is a highly processive actin polymerase tailored to self-assemble parallel-bundled F-actin networks with Fascin

  2014influential reference
• An analysis of splicing, actin-binding properties, heterodimerization and molecular interactions of the non-muscle α-actinins.

  2013influential reference
• Formins at a glance

  2013cited by this paper
• In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics

  2013influential reference
• Integrin-dependent force transmission to the extracellular matrix by α-actinin triggers adhesion maturation

  2013cited by this paper
• α-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly

  2012influential reference
• Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast

  2012cited by this paper
• Contractile‐ring assembly in fission yeast cytokinesis: Recent advances and new perspectives

  2012cited by this paper
• Hierarchical self-organization of cytoskeletal active networks

  2012cited by this paper
• α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction

  2012cited by this paper
• Model of myosin node aggregation into a contractile ring: the effect of local alignment

  2011cited by this paper
• Actin Filament Bundling by Fimbrin Is Important for Endocytosis, Cytokinesis, and Polarization in Fission Yeast*

  2011cited by this paper
• Dividing the Spoils of Growth and the Cell Cycle: The Fission Yeast as a Model for the Study of Cytokinesis

  2011cited by this paper
• Assembly and architecture of precursor nodes during fission yeast cytokinesis

  2011cited by this paper
• Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring

  2011cited by this paper
• α-Actinin-4 Is Essential for Maintaining the Spreading, Motility and Contractility of Fibroblasts

  2010cited by this paper
• Kinetics of myosin node aggregation into a contractile ring.

  2010cited by this paper
• Cytokinesis and the contractile ring in fission yeast: towards a systems-level understanding.

  2010cited by this paper
• Actin Cross-link Assembly and Disassembly Mechanics for α-Actinin and Fascin*

  2010influential reference
• Opening of tandem calponin homology domains regulates their affinity for F-actin

  2010cited by this paper
• Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis.

  2010influential reference
• Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast.

  2010influential reference
• Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile-ring assembly during fission yeast cytokinesis.

  2009cited by this paper
• Role of tropomyosin in formin-mediated contractile ring assembly in fission yeast.

  2009cited by this paper
• The Cytokinesis Formins from the Nematode Worm and Fission Yeast Differentially Mediate Actin Filament Assembly*

  2008cited by this paper
• Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast

  2008influential reference
• α-Actinin structure and regulation

  2008cited by this paper
• Dynamic viscoelasticity of actin cross-linked with wild-type and disease-causing mutant alpha-actinin-4.

  2008influential reference
• Alpha-actinin is required for tightly regulated remodeling of the actin cortical network during cytokinesis.

  2007cited by this paper
• Disease-associated mutant α-actinin-4 reveals a mechanism for regulating its F-actin-binding affinity

  2007cited by this paper
• α-Actinin-4 Is Required for Normal Podocyte Adhesion*

  2007cited by this paper
• Novel structures for alpha-actinin:F-actin interactions and their implications for actin-membrane attachment and tension sensing in the cytoskeleton.

  2007cited by this paper
• Collective Langevin dynamics of flexible cytoskeletal fibers

  2007cited by this paper
• A Comparative and Phylogenetic Analysis of the α-Actinin Rod Domain

  2007cited by this paper
• Three-dimensional arrangement of F-actin in the contractile ring of fission yeast

  2007cited by this paper
• A comparative and phylogenetic analysis of the alpha-actinin rod domain.

  2007cited by this paper
• Stress fibers are generated by two distinct actin assembly mechanisms in motile cells

  2006cited by this paper
• Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast

  2006cited by this paper
• Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin.

  2006cited by this paper
• Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding models.

  2006influential reference
• Characterization of Entamoeba histolytica alpha-actinin.

  2006influential reference
• In vitro assembly of filopodia-like bundles.

  2006cited by this paper
• Characterization of Entamoeba histolytica α-actinin2

  2006cited by this paper
• Mutational and Biological Analysis of alpha-actinin-4 in focal segmental glomerulosclerosis.

  2005cited by this paper
• Spectrin, alpha-actinin, and dystrophin.

  2005cited by this paper
• Actinin-4 increases cell motility and promotes lymph node metastasis of colorectal cancer.

  2005cited by this paper
• Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy.

  2005cited by this paper
• Phosphoinositide binding regulates alpha-actinin dynamics: mechanism for modulating cytoskeletal remodeling.

  2005cited by this paper
• Molecular evolution and structure of alpha-actinin.

  2004cited by this paper
• The many faces of filamin: A versatile molecular scaffold for cell motility and signalling

  2004cited by this paper
• A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region.

  2004cited by this paper
• Molecular Evolution and Structure of α-Actinin

  2004cited by this paper
• UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II

  2004cited by this paper
• Ena/VASP proteins: regulators of the actin cytoskeleton and cell migration.

  2003cited by this paper
• Polarized actin bundles formed by human fascin‐1: their sliding and disassembly on myosin II and myosin V in vitro

  2003cited by this paper
• The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin

  2003cited by this paper
• Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.

  2003cited by this paper
• Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins

  2002cited by this paper
• The three-dimensional structure of alpha-actinin obtained by cryoelectron microscopy suggests a model for Ca(2+)-dependent actin binding.

  2001influential reference
• Crystal Structure of the alpha-Actinin Rod: Four Spectrin Repeats Forming a Thight Dimer.

  2001cited by this paper
• Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis.

  2001influential reference
• Profilin binding to poly-L-proline and actin monomers along with ability to catalyze actin nucleotide exchange is required for viability of fission yeast.

  2001cited by this paper
• Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.

  2001cited by this paper
• Fluorescently-labeled fimbrin decorates a dynamic actin filament network in live plant cells

  2001cited by this paper
• Isoforms of α-Actinin from Cardiac, Smooth, and Skeletal Muscle Form Polar Arrays of Actin Filaments

  2000cited by this paper
• Parallel actin bundles and their multiple actin-bundling proteins.

  2000cited by this paper
• Mutations in ACTN4, encoding α-actinin-4, cause familial focal segmental glomerulosclerosis

  2000cited by this paper
• Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis.

  2000cited by this paper
• Structure of the alpha-actinin rod: molecular basis for cross-linking of actin filaments.

  1999cited by this paper
• CRYSTAL STRUCTURE OF TWO CENTRAL SPECTRIN-LIKE REPEATS FROM ALPHA-ACTININ

  1999cited by this paper
• Dynamic Cross-linking by α-Actinin Determines the Mechanical Properties of Actin Filament Networks*

  1998cited by this paper
• Crystal structure of a calponin homology domain

  1997cited by this paper
• Understanding molecular simulation: from algorithms to applications

  1996cited by this paper
• Actin-bundling proteins.

  1994cited by this paper
• Affinity of alpha-actinin for actin determines the structure and mechanical properties of actin filament gels.

  1993influential reference
• Modular organization of actin crosslinking proteins.

  1991cited by this paper
• Modular organization of actin crosslinking proteins.

  1991cited by this paper
• Bundling of actin filaments by alpha-actinin depends on its molecular length

  1990cited by this paper
• The structure and function of alpha-actinin.

  1989cited by this paper
• Calcium-sensitive non-muscle alpha-actinin contains EF-hand structures and highly conserved regions.

  1987influential reference
• The structure and function of alpha-actinin.

  1987cited by this paper
• The structure and function of α-actinin

  1987cited by this paper
• Stress fiber and cleavage furrow formation in living cells microinjected with fluorescently labeled alpha-actinin.

  1987cited by this paper
• Calcium‐sensitive non‐muscle α‐actinin contains EF‐hand structures and highly conserved regions

  1987cited by this paper
• Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage

  1985cited by this paper
• Alpha-actinin localization in the cleavage furrow during cytokinesis

  1978cited by this paper
• The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

  1971cited by this paper

• Fast actin disassembly and fimbrin mechanosensitivity support rapid turnover during clathrin-mediated endocytosis

  2024cites this paper
• Tuning the contractility and deformation modes of active actin-based assemblies in vitro: from 2D active networks to liquid crystal drops

  2024cites this paper
• Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition

  2024cites this paper
• Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.

  2024cites this paper
• Mechanical positive feedback and biochemical negative feedback combine to generate complex contractile oscillations in cytokinesis

  2023cites this paper
• Actin-binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin II

  2022cites this paper
• Wrangling Actin Assemblies: Actin Ring Dynamics during Cell Wound Repair

  2022cites this paper
• Actin turnover protects the cytokinetic contractile ring from structural instability

  2022cites this paper
• 3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration

  2021cites this paper
• Loss of Septation Initiation Network (SIN) kinases blocks tissue invasion and unlocks echinocandin cidal activity against Aspergillus fumigatus

  2021cites this paper
• Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.

  2021cites this paper
• Contractile ring constriction and septation in fission yeast are integrated mutually stabilizing processes

  2021cites this paper
• Actin cytoskeleton network self-organization through competition and cooperation

  2021cites this paper
• Molecular Mechanism for the Actin-Binding Domain of Alpha-Actinin Ain1 Elucidated by Molecular Dynamics Simulations and Mutagenesis Experiments.

  2020cites this paper
• Pseudomonas aeruginosa exoenzyme Y directly bundles actin filaments

  2020cites this paper
• Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin II

  2020cites this paper
• The Scaffold Proteins Paxillin B and α-Actinin Regulate Septation in Aspergillus nidulans via Control of Actin Ring Contraction

  2020cites this paper
• Mechanics of the cellular actin cortex: From signalling to shape change.

  2020cites this paper
• F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation.

  2020cites this paper
• Cell cycle-dependent phosphorylation of IQGAP is involved in assembly and stability of the contractile ring in fission yeast.

  2020cites this paper
• Actin bundle architecture and mechanics regulate myosin II force generation

  2020cites this paper
• Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin II Running title: Rng2CHD inhibits acto-myosin II movement

  2020cites this paper
• Bond type and discretization of non-muscle myosin II are critical for simulated contractile dynamics

  2019cites this paper
• Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli

  2019cites this paper
• Astral Microtubules Regulate Formation of the Cytokinetic Cleavage Furrow in Fission Yeast

  2019cites this paper
• Molecular form and function of the cytokinetic ring

  2019cites this paper
• Cooperation between tropomyosin and α-actinin inhibits fimbrin association with actin filament networks in fission yeast

  2019cites this paper
• Visualizing dynamic actin cross‐linking processes driven by the actin‐binding protein anillin

  2019cites this paper
• Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast

  2019cites this paper
• Empowering statistical methods for cellular and molecular biologists

  2019cites this paper
• Network Contractility during Cytokinesis—From Molecular to Global Views

  2019cites this paper
• Mechanical and kinetic factors drive sorting of F-actin cross-linkers on bundles

  2018cites this paper
• Ena/VASP processive elongation is modulated by avidity on actin filaments bundled by the filopodia cross-linker fascin

  2018cites this paper
• Alpha-actinin of the chlorarchiniophyte Bigelowiella natans

  2018cites this paper
• Unite to divide – how models and biological experimentation have come together to reveal mechanisms of cytokinesis

  2018cites this paper
• Mechanisms of contractile ring tension production and constriction

  2018cites this paper
• Regulation and Assembly of Actomyosin Contractile Rings in Cytokinesis and Cell Repair

  2018cites this paper
• Involvement of the septation initiation network in events during cytokinesis in fission yeast

  2018cites this paper
• Tuning molecular motor transport through cytoskeletal filament network organization

  2018cites this paper
• Mathematically Modeling the Mechanics of Cell Division

  2018cites this paper
• Mechanical Regulation in Cell Division and in Neurotransmitter Release

  2018cites this paper
• Cross-linkers both drive and brake cytoskeletal remodeling and furrowing in cytokinesis

  2017influential citation
• A node organization in the actomyosin contractile ring generates tension and aids stability

  2017cites this paper
• Molecular dissection of the actin-binding ability of the fission yeast &agr;-actinin, Ain1, in vitro and in vivo

  2017cites this paper
• Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins

  2017cites this paper
• Nanoscale architecture of the Schizosaccharomyces pombe contractile ring

  2017cites this paper
• Tropomyosin and α-actinin cooperation inhibits fimbrin association with actin filament networks in fission yeast

  2017cites this paper
• A Genetic Screen for Fission Yeast Gene Deletion Mutants Exhibiting Hypersensitivity to Latrunculin A

  2016cites this paper