Lactate dehydrogenase of Mugil sp. (Mugilidae, Perciformes). Lack of electrokinetic, thermostability and kinetic differences among individuals with different number of scales

O numero de escamas em series laterais (SNS) de exemplares de Mugil sp. (Mugilidae, Perciformes) coletados na regiao estuarino-lagunar de Cananeia, Estado de Sao Paulo, varia de 33 a 39. A fim de tentar determinar se exemplares com diferentes SNS corresponderiam a diferentes especies ou populacoes de tainhas, foram analisadas as propriedades eletrocineticas, cineticas e de termoestabilidade da sua lactato desidrogenase (LDH). A exemplo de muitos teleosteos, a LDH de Mugil sp. mostrou-se codificada por 3 locos genicos: LDH-A*, LDH-B* e LDH-C*. Metodo de diluicoes seriadas aplicado a 10 diferentes tecidos dessa especie sugeriu um padrao bidirecionalmente divergente de expressao desses locos da LDH. Nao foi observada nenhuma associacao entre padrao eletroforetico, inativacao termica, respostas cineticas e diferentes SNS. Os valores de Km (pyr) aparente aqui obtidos mostraram-se similares aos obtidos por outros autores para musculo e coracao, bem como aos de suas isoformas purificadas. O efeito do NaCl nos valores de Km e Vmax de Mugil sp. (SNS de 35 e 39) indica que esse sal se comporta como um inibidor competitivo, ja que decresce a afinidade enzima-substrato. Assim, os comportamentos eletrocineticos e de termoestabilidade, valores de Km e Vmax e os efeitos do NaCl sobre esses nao nos permitem caracterizar as tainhas capturadas na regiao estuarino-lagunar de Cananeia, com amplitudes de SNS de 33 a 39, como diferentes especies ou populacoes.

• Publication year

  2000

• Venue

  Genetics and Molecular Biology

• Publication date

  2000-03-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1590/S1415-47572000000100026
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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