CTX-M-Type Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Cases of Bovine Mastitis in Japan

N. Saishu,H. Ozaki,T. Murase

Published 2014 in Journal of Veterinary Medical Science

ABSTRACT

ABSTRACT Three Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamase (ESBL) were obtained from three dairy cows with clinical mastitis in two farms in western Japan. Two of the 3 isolates from cows in different farms were able to transfer plasmids carrying the blaCTX-M-2 gene to Escherichia coli recipient. Pulsed-field gel electrophoresis (PFGE) patterns of the 2 isolates were different from each other, although restricted-fragment patterns of the two conjugative plasmids were similar to each other. Additionally, PCR-based replicon typing revealed that both the plasmids belonged to type Inc.T. These results suggest that ESBL-encoding genes can be distributed in bacteria on dairy farms through the plasmids.

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