Permissive sites and topology of an outer membrane protein with a reporter epitope

No abstract is available for this paper.

• Publication year

  1991

• Venue

  Journal of Bacteriology

• Publication date

  1991-01-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1128/jb.173.1.262-275.1991PMID 1702781PMCID PMC207183
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Export and one-step purification from Escherichia coli of a MalE-CD4 hybrid protein that neutralizes HIV in vitro.

  1990cited by this paper
• Analysis of protein localization by use of gene fusions with complementary properties

  1990cited by this paper
• Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria.

  1990cited by this paper
• Alkaline phosphatase fusions: sensors of subcellular location

  1990cited by this paper
• Surface topology of the Escherichia coli K-12 ferric enterobactin receptor

  1990cited by this paper
• Creation of targets for proteolytic cleavage in the LamB protein of E coli K12 by genetic insertion of foreign sequences: implications for topological studies.

  1990cited by this paper
• In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.

  1990cited by this paper
• Crystallization of genetically engineered active maltose-binding proteins, including an immunogenic viral epitope insertion.

  1990cited by this paper
• Induction of virus-neutralizing antibodies by bacteria expressing the C3 poliovirus epitope in the periplasm. The route of immunization influences the isotypic distribution and the biologic activity of the antipoliovirus antibodies.

  1990cited by this paper
• Critical role of neighbouring sequences on the immunogenicity of the C3 poliovirus neutralization epitope expressed at the surface of recombinant bacteria.

  1990cited by this paper
• Introduction of guest peptides into Escherichia coli alkaline phosphatase. Excision and purification of a dynorphin analogue from an active chimeric protein.

  1990cited by this paper
• Immune response to cholera toxin epitope inserted in Salmonella flagellin.

  1989cited by this paper
• Antibodies against synthetic peptides and the topology of LamB, an outer membrane protein from Escherichia coli K12.

  1989cited by this paper
• Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate.

  1988cited by this paper
• Genetic approaches to the study and use of proteins: random point mutations and random linker insertions

  1988cited by this paper
• Maltose transport and starch binding in phage-resistant point mutants of maltoporin. Functional and topological implications.

  1988influential reference
• Genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure

  1988cited by this paper
• Use of outer membrane protein PhoE as a carrier for the transport of a foreign antigenic determinant to the cell surface of Escherichia coli K-12.

  1987cited by this paper
• Silent and functional changes in the periplasmic maltose-binding protein of Escherichia coli K12. I. Transport of maltose.

  1987cited by this paper
• Bacteriophage lambda receptor site on the Escherichia coli K-12 LamB protein

  1987cited by this paper
• Identification and characterization of a continuous neutralization epitope (C3) present on type 1 poliovirus.

  1987cited by this paper
• A vector for the construction of translational fusions to TEM beta-lactamase and the analysis of protein export signals and membrane protein topology.

  1986cited by this paper
• Mutations affecting antigenic determinants of an outer membrane protein of Escherichia coli.

  1986cited by this paper
• Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12

  1986cited by this paper
• Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface.

  1986influential reference
• Sequencing of supercoiled plasmid DNA

  1986cited by this paper
• A genetic approach to analyzing membrane protein topology.

  1986cited by this paper
• Isolation of different bacteriophages using the LamB protein for adsorption on Escherichia coli K-12

  1985cited by this paper
• Molecular basis of bacterial outer membrane permeability.

  1985cited by this paper
• Screening for lamB missense mutations which alter all lambda receptor activities in Escherichia coli K12.

  1984cited by this paper
• Linker Tailing: Unphosphorylated Linker Oligonucleotides for Joining DNA Termini

  1984cited by this paper
• Linker mutagenesis in the gene of an outer membrane protein of escherichia coli, LamB

  1984cited by this paper
• Further sequence analysis of the phage lambda receptor site. Possible implications for the organization of the lamB protein in Escherichia coli K12.

  1984cited by this paper
• The use of a monoclonal antibody and of DNA recombinant technology for the localization of a poliovirus neutralization epitope in viral capsid polypeptide VP1.

  1984cited by this paper
• Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule

  1984cited by this paper
• Topology of phage lambda receptor protein. Mapping targets of proteolytic cleavage in relation to binding sites for phage or monoclonal antibodies.

  1984cited by this paper
• Assembly pathway of newly synthesized LamB protein an outer membrane protein of Escherichia coli K-12.

  1984cited by this paper
• Genetic mapping of antigenic determinants on a membrane protein.

  1983cited by this paper
• Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

  1983cited by this paper
• Mechanisms of protein localization

  1983cited by this paper
• Molecular weights and subunit structure of LamB proteins.

  1982cited by this paper
• The receptor protein of phage lambda: purification, characterization and preliminary electrical studies in planar lipid bilayers.

  1982cited by this paper
• In vivo and in vitro functional alterations of the bacteriophage lambda receptor in lamB missense mutants of Escherichia coli K-12

  1981cited by this paper
• Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12.

  1981cited by this paper
• Interaction of the lamB protein with the peptidoglycan layer in Escherichia coli K12.

  1980cited by this paper
• In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

  1978cited by this paper

• Tools for genome-level recoding of in vivo and in vitro production systems

  2017cites this paper
• CCTOP: a Consensus Constrained TOPology prediction web server

  2015cites this paper
• Cytosolic Selection Systems To Study Protein Stability

  2014cites this paper
• Expediting topology data gathering for the TOPDB database

  2014cites this paper
• the Outer Membrane Protein LamB Glycoprotein-Derived Peptides Inserted in Mediated byTrypanosoma cruzi Surface Adhesion of Escherichia coli to HeLa Cells

  2013cites this paper
• Development of allosteric biosensors for the diagnosis of infectious diseases

  2008cites this paper
• Creating hybrid proteins by insertion of exogenous peptides into permissive sites of a class A β‐lactamase

  2008cites this paper
• Insertional protein engineering for analytical molecular sensing

  2006cites this paper
• Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein.

  2004cites this paper
• Determination of Surface-Exposed, Functional Domains of Gonococcal Transferrin-Binding Protein A

  2004cites this paper
• Molecular interaction between bacteriophage and the gram-negative cell envelope

  2004cites this paper
• A new strategy for the site-specific modification of proteins in vivo.

  2003cites this paper
• Maltodextrin transport through lamb.

  2003cites this paper
• Transposable dual reporters for studying the structure-function relationships in membrane proteins: permissive sites in R. prowazekii ATP/ADP translocase.

  2002cites this paper
• Display of proteins on bacteria.

  2002cites this paper
• Plasmid maintenance and recombinant cell fitness explored in bacterial colonies

  2001cites this paper
• Phage selection for site-specific incorporation of unnatural amino acids into proteins in vivo.

  2001cites this paper
• In vivo and in vitro studies of transmembrane β‐strand deletion, insertion or substitution mutants of the Escherichia coli K‐12 maltoporin

  2000cites this paper
• The biogenesis and assembly of bacterial membrane proteins.

  2000cites this paper
• Novel Topological Features of FhaC, the Outer Membrane Transporter Involved in the Secretion of the Bordetella pertussis Filamentous Hemagglutinin*

  2000cites this paper
• Epitope tagging: general method for tracking recombinant proteins.

  2000cites this paper
• The major subunit ClpG of Escherichia coli CS31A fibrillae as an expression vector for different combinations of two TGEV coronavirus epitopes

  1999cites this paper
• Structural and Functional Roles of the Surface-Exposed Loops of the β-Barrel Membrane Protein OmpA fromEscherichia coli

  1999cites this paper
• Adhesion of Escherichia coli to HeLa Cells Mediated byTrypanosoma cruzi Surface Glycoprotein-Derived Peptides Inserted in the Outer Membrane Protein LamB

  1999cites this paper
• Whole cell- and protein-based biosensors for the detection of bioavailable heavy metals in environmental samples

  1999cites this paper
• Membrane assembly of the Escherichia coli outer membrane protein OmpA: exploring sequence constraints on transmembrane beta-strands.

  1999cites this paper
• Metalloadsorption by Escherichia coliCells Displaying Yeast and Mammalian Metallothioneins Anchored to the Outer Membrane Protein LamB

  1998cites this paper
• Engineering of solvent‐exposed loops in Escherichia coli β‐galactosidase

  1998cites this paper
• Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein.

  1998cites this paper
• Immunogenicity of IpaC-Hybrid Proteins Expressed in the Shigella flexneri 2a Vaccine Candidate SC602

  1998cites this paper
• Insertion Mutagenesis of the Ferric Pyoverdine Receptor FpvA ofPseudomonas aeruginosa: Identification of Permissive Sites and a Region Important for Ligand Binding

  1998cites this paper
• Engineering of solvent-exposed loops in Escherichia coli beta-galactosidase.

  1998cites this paper
• Angel Cebolla and Membrane Protein LamB Metallothioneins Anchored to the Outer Displaying Yeast and Mammalian Cells Escherichia coli

  1998cites this paper
• Distinct mechanisms of antibody‐mediated enzymatic reactivation in β‐galactosidase molecular sensors

  1998cites this paper
• Parameters affecting the immunogenicity of recombinant T cell epitopes inserted into hybrid proteins.

  1997cites this paper
• Topology of the outer membrane phospholipase A of Salmonella typhimurium

  1997cites this paper
• Insertion mutagenesis of the lac repressor and its implications for structure-function analysis

  1997cites this paper
• Two‐dimensional structure of the Opc invasin from Neisseria meningitidis

  1997cites this paper
• Porins of Haemophilus influenzae Type b Mutated in Loop 3 and in Loop 4*

  1997cites this paper
• Modulating the immunological properties of a linear B-cell epitope by insertion into permissive sites of the MalE protein.

  1996cites this paper
• In vivo membrane assembly of split variants of the E.coli outer membrane protein OmpA.

  1996cites this paper
• Analysis of the Erwinia chrysanthemi ferrichrysobactin receptor gene: resemblance to the Escherichia coli fepA-fes bidirectional promoter region and homology with hydroxamate receptors

  1996cites this paper
• X-ray model . epitope tagging and a comparison with the Topology of the membrane protein LamB

  1996cites this paper
• Chimeric proteins.

  1996cites this paper
• Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model

  1996cites this paper
• Enhanced metalloadsorption of bacterial cells displaying poly-His peptides

  1996cites this paper
• Epitope Insertions Define Functional and Topological Features of the Escherichia coli Ferric Enterobactin Receptor (*)

  1995cites this paper
• Biologically displayed random peptides as reagents in mapping protein-protein interactions

  1995cites this paper
• Topological analysis of the Escherichia coli ferrichrome-iron receptor by using monoclonal antibodies

  1995cites this paper
• Live bacterial vectors for mucosal immunization

  1995cites this paper
• The Salmonella ompC gene: structure and use as a carrier for heterologous sequences.

  1995cites this paper
• Antigenic determinants of the OmpC porin from Salmonella typhimurium

  1995cites this paper
• Pseudomonas aeruginosa outer membrane protein OprF as an expression vector for foreign epitopes: the effects of positioning and length on the antigenicity of the epitope.

  1995cites this paper
• An intelligent channel (and more)

  1995cites this paper
• Intracellular processing and presentation of T cell epitopes, expressed by recombinant Escherichia coli and Salmonella typhimurium, to human T cells

  1995cites this paper
• Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution

  1995cites this paper
• Topological analysis of the human beta 2-adrenergic receptor expressed in Escherichia coli.

  1994cites this paper
• Membrane topology and assembly of the outer membrane protein OmpA of Escherichia coli K12

  1994cites this paper
• A role for residue 151 of LamB in bacteriophage lambda adsorption: possible steric effect of amino acid substitutions

  1994cites this paper
• Molecular characterization of the major outer-membrane protein OprF from plant root-colonizing Pseudomonas fluorescens.

  1994cites this paper
• A model of maltodextrin transport through the sugar‐specific porin, LamB, based on deletion analysis.

  1994cites this paper
• Permissive linker insertion sites in the outer membrane protein of 987P fimbriae of Escherichia coli

  1994cites this paper
• Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range

  1994cites this paper
• Molecular and cellular parameters controlling the immunogenicity of foreign B- or T-cell epitopes expressed by recombinant vectors.

  1994cites this paper
• Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12

  1994cites this paper
• Immunogenicity of viral B- and T-cell epitopes expressed in recombinant bacterial proteins.

  1994cites this paper
• Chapter 17 The porin superfamily: diversity and common features

  1994cites this paper
• Chapter 18 Outer membrane proteins of Escherichia coli: mechanism of sorting and regulation of synthesis

  1994cites this paper
• Experimental Determination of the Topography of Membrane Proteins: Lessons from the Nicotinic Acetylcholine Receptor, a Multisubunit Polytopic Protein

  1994cites this paper
• Processing of defined T-cell epitopes after phagocytosis of intact bacteria by macrophages.

  1993cites this paper
• Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida.

  1993cites this paper
• Immunological relatedness of the LamB proteins among members of Enterobacteriaceae.

  1993cites this paper
• Properties of bacteriorhodopsin derivatives constructed by insertion of an exogenous epitope into extra‐membrane loops.

  1993cites this paper
• Compartmentalization of defined epitopes expressed in Escherichia coli has only a minor influence on efficiency of phagocytic processing for presentation by class I and class II major histocompatibility complex molecules to T cells

  1993influential citation
• Linker‐insertion mutagenesis of Pseudomonas aeruginosa outer membrane protein OprF

  1993cites this paper
• The ompS gene of Vibrio cholerae encodes a growth‐phase‐dependent maltoporin

  1993cites this paper
• Insertion derivatives containing segments of up to 16 amino acids identify surface- and periplasm-exposed regions of the FhuA outer membrane receptor of Escherichia coli K-12

  1993cites this paper
• Prediction of membrane‐spanning β‐strands and its application to maltoporin

  1993cites this paper
• Construction and first characterization of two reciprocal hybrids between LamB from Escherichia coli K12 and Klebsiella pneumoniae.

  1993cites this paper
• Immune responses to hybrid maltose-binding proteins.

  1993cites this paper
• Phagocytic processing of bacterial antigens for class I MHC presentation to T cells

  1993cites this paper
• Gene-fusion techniques for determining membrane-protein topology

  1993cites this paper
• Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins.

  1993cites this paper
• THE MALTOSE B REGION IN SALMONELLA TYPHIMURIUM, ESCHERICHIA COLI AND OTHER ENTEROBACTERIACEAE

  1993cites this paper
• Immunodominance of a recombinant T-cell epitope depends on its molecular environment.

  1993cites this paper
• Molecular determinants of channel function.

  1992cites this paper
• Targeting of Drugs 3

  1992cites this paper
• A recombinant cell line expressing a form of the Y13-259 anti-p21ras antibody which binds protein A and may be produced as ascites.

  1992cites this paper
• Molecular and cellular targeting in the expression of foreign polypeptides in bacteria

  1992cites this paper
• Analysis of the Pseudomonas aeruginosa major outer membrane protein OprF by use of truncated OprF derivatives and monoclonal antibodies

  1992cites this paper
• Insertional mutagenesis of Bordetella pertussis adenylate cyclase.

  1992cites this paper
• Bacterial Vectors to Target and/or Purify Polypeptides

  1992cites this paper
• DNA sequence analysis of the lamB gene from Klebsiella pneumoniae: implications for the topology and the pore functions in maltoporin

  1992cites this paper
• Transport and anchoring of beta-lactamase to the external surface of Escherichia coli.

  1992cites this paper
• The cellular location of a foreign B cell epitope expressed by recombinant bacteria determines its T cell-independent or T cell-dependent characteristics.

  1991cites this paper
• Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aro A strain of Salmonella typhimurium; their application as potential immunogens.

  1991cites this paper
• Deletions or duplications in the BtuB protein affect its level in the outer membrane of Escherichia coli

  1991influential citation
• Bacterial vectors to target and/or purify polypeptides: their use in immunological studies.

  1991cites this paper
• Expression of foreign polypeptides at the Escherichia coli cell surface.

  1991cites this paper