Evaluation of a Quantitative DNA Methylation Analysis Technique using Methylation-Sensitive/Dependent Restriction Enzymes and Real-Time PCR

No abstract is available for this paper.

• Publication year

  2006

• Venue

  Epigenetics

• Publication date

  2006-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4161/epi.1.3.3392PMID 17965615
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparative analyses of genomic imprinting and CpG island-methylation in mouse Murr1 and human MURR1 loci revealed a putative imprinting control region in mice.

  2006cited by this paper
• Accurate quantification of DNA methylation using combined bisulfite restriction analysis coupled with the Agilent 2100 Bioanalyzer platform

  2006cited by this paper
• DNA methylation and human disease

  2005cited by this paper
• Methods of epigenetic analysis.

  2004cited by this paper
• A NotI-EcoRV promoter library for studies of genetic and epigenetic alterations in mouse models of human malignancies.

  2004cited by this paper
• A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q.

  2004cited by this paper
• A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus.

  2004cited by this paper
• Genomic imprinting.

  2004cited by this paper
• X-chromosome inactivation: counting, choice and initiation

  2001cited by this paper
• Bisulfite genomic sequencing: systematic investigation of critical experimental parameters

  2001cited by this paper
• CpG-island methylation in aging and cancer.

  2000cited by this paper
• Methyl-specific DNA binding by McrBC, a modification-dependent restriction enzyme.

  2000cited by this paper
• CpG-island methylation in aging and cancer.

  2000cited by this paper
• Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction.

  1999cited by this paper
• Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene.

  1998cited by this paper
• Structure of the imprinted mouse Snrpn gene and establishment of its parental-specific methylation pattern.

  1997cited by this paper
• Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE).

  1997cited by this paper
• Inactive allele-specific methylation and chromatin structure of the imprinted gene U2af1-rs1 on mouse chromosome 11.

  1996cited by this paper
• A paternal–specific methylation imprint marks the alleles of the mouse H19 gene

  1995cited by this paper
• An expanded system of restriction landmark genomic scanning (RLGS Ver. 1.8)

  1995cited by this paper
• Genomic imprinting.

  1988cited by this paper
• Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization

  1977cited by this paper
• World Journal of Emergency Surgery Open Access Experiences with Waterjet Hydrosurgery System in Wound Debridement

  year unknowncited by this paper

• Circulating tumour DNA methylation: unlocking a new chapter in cancer diagnosis and treatment.

  2025cites this paper
• Methyltransferase Detection Based on Target-Responsive Transcription Amplification of Light-Up Aptamer.

  2025cites this paper
• Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.

  2024cites this paper
• Epigenetic modifications of cfDNA in liquid biopsy for the cancer care continuum

  2024cites this paper
• Investigating FGFR2 gene as a blood-based epigenetic biomarker in gastric cancer

  2024influential citation
• Identification of a Transmembrane Protein Involved in Shear Stress Signaling and Hepatocarcinogenesis After a Sustained Virological Response to Hepatitis C Virus

  2023cites this paper
• Advanced Omics Techniques for Understanding Cochlear Genome, Epigenome, and Transcriptome in Health and Disease

  2023cites this paper
• Epigenetic Diversity Underlying Seasonal and Annual Variations in Brown Planthopper (BPH) Populations as Revealed by Methylation- sensitive Restriction Assay

  2023cites this paper
• Rockfish: A transformer-based model for accurate 5-methylcytosine prediction from nanopore sequencing

  2022cites this paper
• Identification of Epigenetic Interactions between MicroRNA-30c-5p and DNA Methyltransferases in Neuropathic Pain

  2022cites this paper
• L1 chimeric transcripts are expressed in healthy brain and their deregulation in glioma follows that of their host locus

  2022cites this paper
• Male-specific coordinated changes in expression of miRNA genes, but not other genes within the DLK1-DIO3 locus in multiple sclerosis.

  2022cites this paper
• Comparative DNA Methylome of Phytoplasma Associated Retrograde Metamorphosis in Sesame (Sesamum indicum L.)

  2022cites this paper
• Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.

  2022cites this paper
• DOK7 CpG hypermethylation in blood leukocytes as an epigenetic biomarker for acquired tamoxifen resistant in breast cancer

  2022cites this paper
• A Comparative Overview of Epigenomic Profiling Methods

  2021cites this paper
• Liquid Biopsy of Methylation Biomarkers in Cell-Free DNA.

  2021cites this paper
• Identification of differentially methylated HpaII sites by NGS analysis of HpaII-digested libraries.

  2020cites this paper
• Adverse outcome pathways for ionizing radiation and breast cancer involve direct and indirect DNA damage, oxidative stress, inflammation, genomic instability, and interaction with hormonal regulation of the breast

  2020cites this paper
• Methylation-Sensitive Restriction Enzyme Quantitative Polymerase Chain Reaction Enables Rapid, Accurate, and Precise Detection of Methylation Status of the Regulatory T Cell (Treg)-Specific Demethylation Region in Primary Human Tregs

  2020cites this paper
• Detection of LINE-1 hypomethylation in cfDNA of Esophageal Adenocarcinoma Patients

  2020cites this paper
• Induced pluripotent stem cell reprogramming‐associated methylation at the GABRA2 promoter and chr4p12 GABAA subunit gene expression in the context of alcohol use disorder

  2020cites this paper
• A three-gene methylation marker panel for the nodal metastatic risk assessment of muscle-invasive bladder cancer

  2019cites this paper
• GRSF1 is an age-related regulator of senescence

  2019cites this paper
• Methylation levels of IGF2 and KCNQ1 in spermatozoa from infertile men are associated with sperm DNA damage

  2019cites this paper
• Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM

  2018cites this paper
• Exogenous ABA and endogenous ABA affects ‘Kyoho’ grape berry coloration in different pathway

  2018cites this paper
• Detection of DNA Methylation by Dnmt3a Methyltransferase using Methyl-Dependent Restriction Endonucleases

  2018cites this paper
• Investigating Methylation changes of Vimentin gene in Breast Cancer

  2018cites this paper
• Maternal fructose induces gender-dependent changes in both LXRα promoter methylation and cholesterol metabolism in progeny.

  2018cites this paper
• Epigenetic Changes in Chronic Inflammatory Diseases.

  2017cites this paper
• Epigenetic memory effects in forest trees: a victory of “Michurinian biology”?

  2017cites this paper
• Molecular Techniques for DNA Methylation Studies

  2017cites this paper
• A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands.

  2017cites this paper
• A refined DNA methylation detection method using MspJI coupled quantitative PCR.

  2017influential citation
• Absolute quantification of DNA methylation using microfluidic chip-based digital PCR.

  2017cites this paper
• Etude de l'impact de la dérégulation transcriptionnelle liée à des transcrits chimères initiés à partir d'éléments répétés de type LINE-1 dans la tumorigenèse gliale

  2017influential citation
• An optimized rapid bisulfite conversion method with high recovery of cell-free DNA

  2017cites this paper
• Modeling Neurodevelopmental Disorders Involving Genomic Imprinting at Human Chromosome 15q11-q13 Using iPSC and CRISPR/Cas9 Technology

  2016cites this paper
• A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction.

  2016cites this paper
• Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.)

  2016cites this paper
• Modeling Genomic Imprinting Disorders Using Induced Pluripotent Stem Cells.

  2016cites this paper
• P1 Epigenetic Regulation in Leaves of High Altitude Maize Landraces: Effect of UV-B Radiation

  2016cites this paper
• FGF2 Overrides TGFβ1‐Driven Integrin ITGA11 Expression in Human Dermal Fibroblasts

  2016cites this paper
• The cancer-promoting gene fatty acid-binding protein 5 (FABP5) is epigenetically regulated during human prostate carcinogenesis.

  2016cites this paper
• Essential Role of DNA Methyltransferase 1–mediated Transcription of Insulin-like Growth Factor 2 in Resistance to Histone Deacetylase Inhibitors

  2016cites this paper
• Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.

  2016cites this paper
• Epigenetic Changes during Hepatic Stellate Cell Activation

  2015cites this paper
• Multiplexed locus-specific analysis of DNA methylation in single cells

  2015cites this paper
• High fructose consumption induces DNA methylation at PPARα and CPT1A promoter regions in the rat liver.

  2015cites this paper
• NNK-Induced DNA Methyltransferase 1 in Lung Tumorigenesis in A/J Mice and Inhibitory Effects of (−)-Epigallocatechin-3-Gallate

  2015cites this paper
• Modulation of imprinted gene expression following superovulation.

  2014cites this paper
• Circadian behavior is light-reprogrammed by plastic DNA methylation

  2014cites this paper
• Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

  2014influential citation
• Hepatic Methionine Homeostasis Is Conserved in C57BL/6N Mice on High-Fat Diet Despite Major Changes in Hepatic One-Carbon Metabolism

  2013cites this paper
• Ecological Epigenetics: Beyond MS-AFLP.

  2013cites this paper
• Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

  2013cites this paper
• Haploinsufficiency of the paternal-effect gene Dnmt3L results in transient DNA hypomethylation in progenitor cells of the male germline.

  2013influential citation
• Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine

  2013cites this paper
• Substrate specificity and properties of methyl-directed site-specific DNA endonucleases

  2013cites this paper
• DNA methylation analysis.

  2013cites this paper
• Evidence for regulation of UDP‐glucuronosyltransferase (UGT) 1A1 protein expression and activity via DNA methylation in healthy human livers

  2013cites this paper
• Stability of DNA Methylation Patterns in Mouse Spermatogonia Under Conditions of MTHFR Deficiency and Methionine Supplementation1

  2013influential citation
• Selective induction of glutathione S-transferases in round spermatids from the Brown-Norway rat by the chemotherapeutic regimen for testicular cancer.

  2013cites this paper
• Characterization of Undermethylated Sites in Vibrio cholerae

  2013cites this paper
• Expression and genomic structure of the dormancy-associated MADS box genes MADS13 in Japanese pears (Pyrus pyrifolia Nakai) that differ in their chilling requirement for endodormancy release.

  2013cites this paper
• INHIBITION OF LUNG TUMORIGENESIS AND CANCER CELL GROWTH BY EGCG AND OTHER CHEMOPREVENTIVE AGENTS by

  2013cites this paper
• Murine one-carbon metabolism in non-alcoholic fatty liver disease

  2013cites this paper
• Stability of DNA Methylation Patterns in Mouse Spermatogonia Under Conditions of MTHFR Deficiency and Methionine Supplementation 1 Running Title: DNA methylation in MTHFR-deficient male germ cells

  2013cites this paper
• DNA methyltransferase 1 (Dnmt1) mutation affects Snrpn imprinting in the mouse male germ line.

  2012cites this paper
• Epigenetic alterations in sperm DNA associated with testicular cancer treatment.

  2012cites this paper
• Differential DNA Methylation Alterations in Radiation-Sensitive and -Resistant Cells

  2012cites this paper
• Identification de marqueurs de pronostic impliqués dans l'angiogenèse et régulés par un mécanisme épigénétique dans le cancer du sein

  2011cites this paper
• Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction.

  2011cites this paper
• Quantitative methylation analysis of multiple genes using methylation-sensitive restriction enzyme-based quantitative PCR for the detection of hepatocellular carcinoma.

  2011cites this paper
• Effects of Folic Acid Supplementation on Human Leukocyte DNA Methylation and Gene Expression

  2011cites this paper
• Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ERα/ERβ Balance in Female Mice

  2011cites this paper
• The effects of postovulatory aging of mouse oocytes on methylation and expression of imprinted genes at mid-term gestation.

  2011cites this paper
• Critical Period of Nonpromoter DNA Methylation Acquisition during Prenatal Male Germ Cell Development

  2011cites this paper
• Mutator transposon activation after UV-B involves chromatin remodeling

  2010cites this paper
• Epigenetic basis for aberrant upregulation of autoantigen genes in humans with ANCA vasculitis.

  2010cites this paper
• Quantitative DNA methylation analysis of laser capture microdissected formalin-fixed and paraffin-embedded tissues.

  2010cites this paper
• Quantitative 1-step DNA methylation analysis with native genomic DNA as template.

  2010cites this paper
• Toxicants and human sperm chromatin integrity.

  2010cites this paper
• Chapter 14 – Molecular Techniques for DNA Methylation Studies

  2010cites this paper
• Strain-specific defects in testicular development and sperm epigenetic patterns in 5,10-methylenetetrahydrofolate reductase-deficient mice.

  2010cites this paper
• Method for screening of gene mutation by utilizing quantification technique

  2009cites this paper
• Human DAZL, DAZ and BOULE genes modulate primordial germ cell and haploid gamete formation

  2009cites this paper
• A novel method for sensitive and specific detection of DNA methylation biomarkers based on DNA restriction during PCR cycling.

  2009cites this paper
• Reproductive and epigenetic outcomes associated with aging mouse oocytes.

  2009cites this paper
• Restriction digestion and real-time PCR (qAMP).

  2009cites this paper
• Epigenetic modification of the gene for the vitamin B(12) chaperone MMACHC can result in increased tumorigenicity and methionine dependence.

  2009cites this paper
• Biomarqueur de méthylation pour une détection précoce du cancer gastrique

  2008cites this paper
• Developmental acquisition of genome-wide DNA methylation occurs prior to meiosis in male germ cells.

  2007influential citation
• Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L

  2007cites this paper
• A unique configuration of genome-wide DNA methylation patterns in the testis

  2007cites this paper
• Adverse Effects of 5-Aza-2′-Deoxycytidine on Spermatogenesis Include Reduced Sperm Function and Selective Inhibition of de Novo DNA Methylation

  2007cites this paper