Uniformity of metabolic enzymes within individual motor units

Individual muscle fibers of 10 motor units from the tibialis posterior muscle of cat were identified by glycogen depletion techniques, characterized for histochemical type, diameter, and intramuscular locations, and analyzed by quantitative biochemical methods. Four enzymes, representing different energy-yielding pathways, were quantitatively assayed in muscle fibers belonging to motor units selected from each of the three major physiological types. All four enzymes demonstrated identical activities among fibers within a motor unit, while showing up to 11-fold differences among fibers belonging to different motor units. Moreover, fibers within a single motor unit, but of substantially different diameters, were nevertheless homogeneous in specific enzyme activities.

• Publication year

  1986

• Venue

  Journal of Neuroscience

• Publication date

  1986-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.06-03-00892.1986PMID 3958798PMCID PMC6568454
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Muscle fibers within a single motor unit that differ substantially in diameter remain homogeneous in specific enzyme activities.

  Confidence 0.90

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• All four metabolic enzymes assayed showed identical activities among muscle fibers within a single motor unit, while exhibiting up to 11-fold differences across fibers belonging to different motor units.

  Confidence 0.95

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• glycogen depletion technique

  method

  A histochemical method used to identify which individual muscle fibers belong to a given motor unit by depleting their glycogen stores through stimulation.

  Aliases: glycogen depletion

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• metabolic enzymes

  molecule, measured variable

  Four enzymes representing different energy-yielding pathways that were quantitatively assayed in individual muscle fibers.

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• motor unit

  biological structure

  A functional unit consisting of a motor neuron and all the muscle fibers it innervates, here selected from three major physiological types in cat tibialis posterior muscle.

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• muscle fiber diameter

  measured variable

  The cross-sectional size of individual muscle fibers, measured as a histochemical characterization parameter.

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• muscle fibers

  biological structure

  Individual contractile cells of skeletal muscle, identified here by glycogen depletion techniques and characterized by histochemical type, diameter, and intramuscular location.

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• specific enzyme activity

  measured variable

  The quantitative biochemical measure of enzymatic activity per unit in a muscle fiber sample.

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• tibialis posterior muscle

  biological structure

  A hindlimb muscle of the cat from which motor units and their constituent fibers were sampled.

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