Minimizing cellulase inhibition of whole slurry biomass hydrolysis through the addition of carbocation scavengers during acid-catalyzed pretreatment.

The aim of this work was to study how to minimize cellulase inhibition of whole slurry biomass hydrolysis through addition of carbocation scavengers during acid-catalyzed pretreatment. Various potential carbocation scavengers were compared and their inhibition mitigating effects towards the hydrolytic performance of cellulase enzymes was assessed. The results indicated that the addition of carbocation scavengers during the pretreatment process could not only alleviate the inhibitory effect of the phenolics on the enzymatic hydrolysis but also increase the accessibility of cellulases to the pretreated substrates. It appeared that lignin-derived compounds such as 4-hydroxybenzoic acid, vanillic acid, syringic acid could all serve as efficient scavengers to alleviate the inhibitory effect of phenolics on cellulose hydrolysis where the syringic acid showed the best mitigating effect. By combining the carbocation scavengers in the pretreatment process, an improved cellulose hydrolysis of the pretreated whole slurry could be achieved without any post detoxification step.

• Publication year

  2018

• Venue

  Bioresource Technology

• Publication date

  2018-06-01

• Fields of study

  Medicine, Chemistry, Engineering, Environmental Science

• Identifiers
  DOI 10.1016/j.biortech.2018.02.124PMID 29518686
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Extent of Enzyme Inhibition by Phenolics Derived from Pretreated Biomass Is Significantly Influenced by the Size and Carbonyl Group Content of the Phenolics

  2018cited by this paper
• Quenching of reactive intermediates during mechanochemical depolymerization of lignin

  2018cited by this paper
• Application potential of a carbocation scavenger in autohydrolysis and dilute acid pretreatment to overcome high softwood recalcitrance

  2017cited by this paper
• The effect of p-nitrophenol toward the structural characteristics and antioxidant activity of oil palm fronds (OPF) lignin polymers.

  2017cited by this paper
• Modification of oil palm fronds lignin by incorporation of m-cresol for improving structural and antioxidant properties.

  2017cited by this paper
• Can we enhance cellulose hydrolysis by minimizing enzyme inhibition resulting from pretreatment-derived inhibitors?

  2017cited by this paper
• Understanding the structural and chemical changes of plant biomass following steam explosion pretreatment

  2017cited by this paper
• What Are the Major Components in Steam Pretreated Lignocellulosic Biomass That Inhibit the Efficacy of Cellulase Enzyme Mixtures

  2016cited by this paper
• Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects.

  2016cited by this paper
• Difference analysis of the enzymatic hydrolysis performance of acid-catalyzed steam-exploded corn stover before and after washing with water

  2016cited by this paper
• The accessible cellulose surface influences cellulase synergism during the hydrolysis of lignocellulosic substrates.

  2015cited by this paper
• Ozone detoxification of steam-pretreated Norway spruce

  2015cited by this paper
• Pilot scale study on steam explosion and mass balance for higher sugar recovery from rice straw.

  2015cited by this paper
• Structural Transformation of Isolated Poplar and Switchgrass Lignins during Dilute Acid Treatment

  2015cited by this paper
• Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation

  2015cited by this paper
• Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology

  2013cited by this paper
• Pilot-scale study on the acid-catalyzed steam explosion of rice straw using a continuous pretreatment system.

  2013cited by this paper
• Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose

  2012cited by this paper
• Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents.

  2011cited by this paper
• Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates

  2011cited by this paper
• Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass.

  2011cited by this paper
• Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation.

  2010cited by this paper
• The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis

  2010cited by this paper
• Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review.

  2010cited by this paper
• Laccase detoxification of steam-exploded wheat straw for second generation bioethanol.

  2009cited by this paper
• The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique

  2008cited by this paper
• Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose.

  2008cited by this paper
• Improved lignin properties and reactivity by modifications in the autohydrolysis process of aspen wood

  2008cited by this paper
• Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production

  2007cited by this paper
• A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover

  2007cited by this paper
• Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion.

  2007cited by this paper
• Optimization of SO2-catalyzed steam pretreatment of corn fiber for ethanol production

  2003cited by this paper
• A ninhydrin-based assay to quantitate the total protein content of tissue samples.

  2001cited by this paper
• Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips

  2000cited by this paper
• Fermentability of the hemicellulose-derived sugars from steam-exploded softwood (douglas fir)

  1999cited by this paper
• Condensation reactions of softwood and hardwood lignin model compounds under organic acid cooking conditions

  1997cited by this paper
• Characterization of autohydrolysis aspen (P. tremuloides) lignins. Part 1. Composition and molecular weight distribution of extracted autohydrolysis lignin

  1979cited by this paper
• Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents

  1965cited by this paper

• A ternary deep eutectic solvent for efficient biomass fractionation and lignin stabilization.

  2025cites this paper
• Effects of Nucleophilic Polymer Assistance in Acidic Pretreatment on the Enzyme Digestibility and Fermentability of Pretreated Lignocelluloses

  2025cites this paper
• Optimization of Enzymatic Saccharification of Pretreated Sugarcane Bagasse for Enhanced Polyhydroxybutyrate (PHB) Production by Bacillus megaterium PNCM 1890

  2025cites this paper
• Effect of different bulking agents on the quality, microbial community structure and metabolic functions during human feces composting in foam composting device

  2025cites this paper
• A review on recent developments of oil palm solid waste upcycling into green bio-based materials for sustainable building and construction applications

  2025cites this paper
• Carbocation Scavenger Assisted Diol Organosolv Pretreatment toward Efficient Biomass Saccharification and Lignin Valorization

  2025cites this paper
• Carbocation scavenger enhanced pretreatment of wood for enzymatic saccharification – Experimental study and review

  2025cites this paper
• Strategies for efficient degradation of lignocellulose from straw: Synergistic effects of acid-base pretreatment and functional microbial agents in composting

  2025cites this paper
• Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose

  2024cites this paper
• Role of lignin removal on the properties of crude pulp fibers from corn stover via high-temperature formic acid pulping.

  2024cites this paper
• Sustainable Pathway for Bagasse Conversion Using Continuous Lactic Acid Catalysis: Production of Xylo-Oligosaccharides, Lignin Nanoparticles, and Dissolving Pulp

  2024cites this paper
• Sustainable wheat straw pretreatment process by self-produced and cyclical crude lactic acid.

  2024cites this paper
• Overcoming Softwood Recalcitrance by Improved 2-Naphthol Addition to Steam Explosion Pretreatment

  2024cites this paper
• Comparison of effects of carbocation scavengers assisted acid pretreatment on improving enzymatic digestibility of masson pine and poplar

  2024cites this paper
• Tailoring whole slurry bioprocessing for sugary stovers to augment sugar production by integrating soluble and insoluble carbohydrates

  2024cites this paper
• Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3-Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates.

  2024cites this paper
• Binary additives for in-situ mitigating the inhibitory effect of lignin-derived phenolics on enzymatic hydrolysis of lignocellulose: Enhanced performance and synergistic mechanism

  2023cites this paper
• Using citric acid to suppress lignin repolymerization in the organosolv pretreatment of corn stalk

  2023cites this paper
• Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw.

  2023cites this paper
• Effect of Various Aromatic Compounds with Different Functional Groups on Enzymatic Hydrolysis of Microcrystalline Cellulose and Alkaline Pretreated Wheat Straw

  2023cites this paper
• Co-production of pigment and high value-added bacterial nanocellulose from Suaeda salsa biomass with improved efficiency of enzymatic saccharification and fermentation

  2023cites this paper
• Reduced Recalcitrance and Improved Pulp Properties in Eucalypt Woods Pretreated with White-Rot Fungi and Mild Alkali

  2023cites this paper
• Novel Pre-treatment for Lignocellulosic Biomass Delignification Using Alkaline-Assisted Ohmic Heating

  2023cites this paper
• Co-production of xylooligosaccharides, glucose, and less-condensed lignin from sugarcane bagasse using syringic acid pretreatment.

  2023cites this paper
• Novel dual-action vanillic acid pretreatment for efficient hemicellulose separation with simultaneous inhibition of lignin condensation.

  2023cites this paper
• Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies.

  2022cites this paper
• Alleviating lignin repolymerization by carbocation scavenger for effective production of fermentable sugars from combined liquid hot water and green-liquor pretreated softwood biomass

  2022cites this paper
• Steam explosion of lignocellulosic biomass for multiple advanced bioenergy processes: A review

  2022cites this paper
• Production of Itaconic Acid by Aspergillus terreus from Sorghum Bran Hydrolysates and Optimization for Fermentative Production

  2022cites this paper
• 2-Naphthol Impregnation Prior to Steam Explosion Promotes LPMO-Assisted Enzymatic Saccharification of Spruce and Yields High-Purity Lignin

  2022cites this paper
• Valorisation Potential of Invasive Acacia dealbata, A. longifolia and A. melanoxylon from Land Clearings

  2022cites this paper
• A Critical Review on the Effect of Lignin Redeposition on Biomass in Controlling the Process of Enzymatic Hydrolysis

  2022cites this paper
• Recent advances in the valorization of plant biomass

  2021cites this paper
• An effective strategy for improving the specific activity and saccharification efficiency of cellulase by pre-incubation with phenolic acids.

  2021cites this paper
• Emerging approaches in lignocellulosic biomass pretreatment and anaerobic bioprocesses for sustainable biofuels production

  2021cites this paper
• A review on physico-chemical delignification as a pretreatment of lignocellulosic biomass for enhanced bioconversion.

  2021cites this paper
• Nuclear magnetic resonance analysis of ascorbic acid assisted lignocellulose decomposition in dilute acid pretreatment and its stimulation on enzymatic hydrolysis.

  2021cites this paper
• Identifying the negative cooperation between major inhibitors of cellulase activity and minimizing their inhibitory potential during hydrolysis of acid-pretreated corn stover.

  2021cites this paper
• Acid-catalyzed steam explosion for high enzymatic saccharification and low inhibitor release from lignocellulosic cardoon stalks

  2021cites this paper
• Modification of lignin by various additives to mitigate lignin inhibition for improved enzymatic digestibility of dilute acid pretreated hardwood

  2021cites this paper
• Using nucleophilic naphthol derivatives to suppress biomass lignin repolymerization in fermentable sugar production

  2021cites this paper
• Eco-friendly additives in acidic pretreatment to boost enzymatic saccharification of hardwood for sustainable biorefinery applications

  2021cites this paper
• Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments

  2021cites this paper
• Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches

  2021cites this paper
• Organosolv pretreatment assisted by carbocation scavenger to mitigate surface barrier effect of lignin for improving biomass saccharification and utilization

  2021cites this paper
• Tailoring Lignin Structure to Maximize the Value from Lignin

  2021cites this paper
• Facilitating enzymatic digestibility of larch by in-situ lignin modification during combined acid and alkali pretreatment.

  2020cites this paper
• Enzymatic conversion of pretreated lignocellulosic biomass: A review on influence of structural changes of lignin.

  2020cites this paper
• Improving Enzymatic Saccharification and Ethanol Production from Hardwood by Deacetylation and Steam Pretreatment: Insight into Mitigating Lignin Inhibition

  2020cites this paper
• Overview of pretreatment methods employed on oil palm biomass in producing value-added products: A review

  2020cites this paper
• Green biorefinery — the ultra-high hydrolysis rate and behavior of Populus tomentosa hemicellulose autohydrolysis under moderate subcritical water conditions

  2020cites this paper
• Understanding the structural characteristics of water-soluble phenolic compounds from four pretreatments of corn stover and their inhibitory effects on enzymatic hydrolysis and fermentation

  2020cites this paper
• Improving enzymatic hydrolysis of acid-pretreated bamboo residues using amphiphilic surfactant derived from dehydroabietic acid.

  2019cites this paper
• Improving enzymatic saccharification of hardwood through lignin modification by carbocation scavengers and the underlying mechanisms.

  2019cites this paper
• Boosting Ethanol Productivity of Zymomonas mobilis 8b in Enzymatic Hydrolysate of Dilute Acid and Ammonia Pretreated Corn Stover Through Medium Optimization, High Cell Density Fermentation and Cell Recycling

  2019cites this paper
• Integrated process for the coproduction of fermentable sugars and lignin adsorbents from hardwood.

  2019cites this paper