Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Biochemistry

• Publication date

  2011-04-12

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/bi2001032PMID 21351740PMCID PMC3071448
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues .

  2010cited by this paper
• Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase.

  2009cited by this paper
• Mimicking phosphorylation of Ser‐74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues

  2008cited by this paper
• Elucidation of different binding modes of purine nucleosides to human deoxycytidine kinase.

  2008cited by this paper
• Structural basis for substrate promiscuity of dCK.

  2008cited by this paper
• Functional studies of active‐site mutants from Drosophila melanogaster deoxyribonucleoside kinase

  2007cited by this paper
• Nonenantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with L- and D-nucleosides.

  2007cited by this paper
• Fluorescence energy transfer studies of human deoxycytidine kinase: role of cysteine 185 in the conformational changes that occur upon substrate binding.

  2006cited by this paper
• Structural basis for the preference of UTP over ATP in human deoxycytidine kinase: illuminating the role of main-chain reorganization.

  2006cited by this paper
• Structural basis for activation of the therapeutic l-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase

  2006cited by this paper
• Identification of Phosphorylation Sites on Human Deoxycytidine Kinase After Overexpression in Eucaryotic Cells

  2006cited by this paper
• Identification of in Vivo Phosphorylation Sites on Human Deoxycytidine Kinase

  2006cited by this paper
• Activation of deoxycytidine kinase in lymphocytes is calcium dependent and involves a conformational change detectable by native immunostaining.

  2004cited by this paper
• Structure of human dCK suggests strategies to improve anticancer and antiviral therapy

  2003cited by this paper
• A pre-steady-state kinetic analysis of substrate binding to human recombinant deoxycytidine kinase: a model for nucleoside kinase action.

  1999cited by this paper
• Kinetic analysis of human deoxycytidine kinase with the true phosphate donor uridine triphosphate.

  1997cited by this paper
• MOLREP: an Automated Program for Molecular Replacement

  1997cited by this paper
• Refinement of macromolecular structures by the maximum-likelihood method.

  1997cited by this paper
• Properties and levels of deoxynucleoside kinases in normal and tumor cells; implications for chemotherapy.

  1994cited by this paper
• Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants

  1993cited by this paper
• Nucleotide specificity of human deoxycytidine kinase.

  1992cited by this paper
• Guanylate kinases from human erythrocytes, hog brain, and rat liver.

  1978cited by this paper

• ActivityFinder: Toward the Fully Automatic Integration of Structural and Binding Affinity Data

  2026cites this paper
• Optimization and evaluation of complementary degrader discovery assays for application in screening

  2025cites this paper
• From a drug repositioning to a structure-based drug design approach to tackle acute lymphoblastic leukemia

  2023cites this paper
• Hypertension: Constraining the Expression of ACE-II by Adopting Optimal Macronutrients Diet Predicted via Support Vector Machine

  2022cites this paper
• Enzyme-Nanoparticle Corona: A Novel Approach, Their Plausible Applications and Challenges

  2021cites this paper
• In silico modeling revealed new insights into the mechanism of action of enzyme 2'-5'-oligoadenylate synthetase in cattle

  2021cites this paper
• The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs

  2020cites this paper
• Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer

  2019cites this paper
• Defective Nucleotide Catabolism Defines a Subset of Cancers Sensitive to Purine Nucleoside Phosphorylase Inhibition

  2019cites this paper
• Cadherin profiling for therapeutic interventions in Epithelial Mesenchymal Transition (EMT) and tumorigenesis

  2018cites this paper
• Oleaginous Yeasts: Lignocellulosic Biomass Derived Single Cell Oil as Biofuel Feedstock

  2018cites this paper
• Microbial CYP450: An Insight into Its Molecular/Catalytic Mechanism, Production and Industrial Application

  2018cites this paper
• Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells.

  2018cites this paper
• Negative Cooperative Binding of Thymidine, Ordered Substrate Binding, and Product Release of Human Mitochondrial Thymidine Kinase 2 Explain Its Complex Kinetic Properties and Physiological Functions

  2018cites this paper
• In silico pharmacodynamics, toxicity profile and biological activities of the Saharan medicinal plant Limoniastrum feei

  2017cites this paper
• Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology

  2017cites this paper
• Double approche à la thérapie anti-tumorale à l'aide de vecteurs lentiviraux

  2017cites this paper
• ATR inhibition facilitates targeting of leukemia dependence on convergent nucleotide biosynthetic pathways

  2017cites this paper
• The Role of Deoxycytidine Kinase (dCK) in Radiation-Induced Cell Death

  2016cites this paper
• Cellules pour l'immunothérapie modifiées afin de cibler l'antigène cd38 et pour inactiver le gène cd38

  2016cites this paper
• Non-Viral Deoxyribonucleoside Kinases--Diversity and Practical Use.

  2015cites this paper
• Selective Phosphorylation of South and North-Cytidine and Adenosine Methanocarba-Nucleosides by Human Nucleoside and Nucleotide Kinases Correlates with Their Growth Inhibitory Effects on Cultured Cells

  2015cites this paper
• Biochemical Characterization of Human Guanylate Kinase and Mitochondrial Thymidine Kinase: Essential Enzymes for the Metabolic Activation of Nucleoside Analog Prodrugs

  2015cites this paper
• Potent Sensitisation of Cancer Cells to Anticancer Drugs by a Quadruple Mutant of the Human Deoxycytidine Kinase

  2015cites this paper
• A Phosphotyrosine Switch for Cargo Sequestration at Clathrin-coated Buds*

  2014cites this paper
• Deoxycytidine Kinase Augments ATM-Mediated DNA Repair and Contributes to Radiation Resistance

  2014cites this paper
• Structural characterization of new deoxycytidine kinase inhibitors rationalizes the affinity-determining moieties of the molecules.

  2014cites this paper
• Protein phosphatase 2A regulates deoxycytidine kinase activity via Ser‐74 dephosphorylation

  2014cites this paper
• Mechanistic Evidence to Support the Anti-hepatitis B Viral Activity of Multifunctional Scaffold & Conformationally Restricted Magnolol

  2014cites this paper
• Synthese und biologische Evaluierung neurotropher Substanzkollektionen

  2014cites this paper
• Mechanistic Evidence to Support the Anti-hepatitis B Viral Activity of Multifunctional Scaffold & Conformationally Restricted Magnolol

  2014cites this paper
• Mechanistic studies on the synergistic cytotoxicity of the nucleoside analogs gemcitabine and clofarabine in multiple myeloma: relevance of p53 and its clinical implications.

  2013cites this paper
• New insights into the synergism of nucleoside analogs with radiotherapy

  2013cites this paper
• Studies of enzymes in mitochondrial DNA precursor synthesis

  2013cites this paper
• Phosphorylation of deoxycytidine kinase on Ser-74: impact on kinetic properties and nucleoside analog activation in cancer cells.

  2012cites this paper
• Retrovolution: HIV–Driven Evolution of Cellular Genes and Improvement of Anticancer Drug Activation

  2012cites this paper
• Development of Novel Cell Fate Control Gene Therapy for Applications in Cancer and Immune Disorders

  2012cites this paper
• Doctor of Philosophy in Molecular and Medical Pharmacology

  2012cites this paper
• Cell fate control gene therapy based on engineered variants of human deoxycytidine kinase.

  2012cites this paper
• Structure-guided Engineering of Human Thymidine Kinase 2 as a Positron Emission Tomography Reporter Gene for Enhanced Phosphorylation of Non-natural Thymidine Analog Reporter Probe*

  2011cites this paper