Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.

A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.

• Publication year

  1983

• Venue

  Journal of Biological Chemistry

• Publication date

  1983-11-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(17)43925-1PMID 6417125
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

  1983cited by this paper
• Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

  1983cited by this paper
• Positive-selection cloning vehicle useful for overproduction of hybrid proteins

  1983cited by this paper
• Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging.

  1982cited by this paper
• Immunological studies of RNA polymerase II using antibodies to subunits of Drosophila and wheat germ enzyme.

  1982cited by this paper
• Properties of mutationally altered RNA polymerases II of Drosophila.

  1982cited by this paper
• A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

  1981cited by this paper
• Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.

  1980cited by this paper
• Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster

  1980cited by this paper
• Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II.

  1979cited by this paper
• Temperature-sensitive RNA polymerase II mutations in Chinese hamster ovary cells.

  1978cited by this paper
• Identification of the amatoxin-binding subunit of RNA polymerase B by affinity labeling experiments. Subunit B3-the true amatoxin receptor protein of multiple RNA polymerase B.

  1976cited by this paper
• Animal DNA-dependent RNA polymerases. 11. Mechanism of the inhibition of RNA polymerases B by amatoxins.

  1974cited by this paper

• Kinetic measurement of gene-specific RNA polymerase II transcription elongation rates

  2025cites this paper
• Alpha‐amanitin tolerance of adult flies of mycophagous and nonmycophagous species in Drosophilidae

  2024cites this paper
• Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin

  2023cites this paper
• RNA polymerase II speed: a key player in controlling and adapting transcriptome composition

  2021cites this paper
• In silico identification of Drosophila melanogaster genes encoding RNA polymerase subunits

  2020cites this paper
• Host use and host shifts in Drosophila.

  2019cites this paper
• The Cyclic Peptide Toxins of Amanita and Other Poisonous Mushrooms

  2018cites this paper
• Biological Activities of the Amanita Peptide Toxins

  2018cites this paper
• melanogaster RpII215. Drosophila element deletions in the 5' region of Sites of P element insertion and structures of P

  2013cites this paper
• Phenotypic Analysis of Schizosaccharomyces Pombe Strains bearing Site-Directed Mutations in the Carboxy Terminal Domain of the Largest Subunit of RNA Polymerase II

  2012cites this paper
• Distribution and Mechanism of &agr;-Amanitin Tolerance in Mycophagous Drosophila (Diptera: Drosophilidae)

  2011cites this paper
• RNA polymerase active center: the molecular engine of transcription.

  2009cites this paper
• On the early evolution of RNA polymerase

  2005cites this paper
• Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila

  2004cites this paper
• Structure and Functions of Eukaryotic Nuclear DNA-Dependent RNA Polymerase I

  2004cites this paper
• RNA polymerase II transcripts as targets for 5-fluorouridine cytotoxicity: antagonism of 5-fluorouridine actions by α-amanitin

  2004cites this paper
• Clonal analysis of two mutations in the large subunit of RNA polymerase II of Drosophila

  2004cites this paper
• Mutational analysis of Drosophila RNA polymerase II.

  2003cites this paper
• Intrinsic Transcript Cleavage in Yeast RNA Polymerase II Elongation Complexes*

  2003cites this paper
• Toxins of Amanita phalloides.

  1998cites this paper
• Selection and analysis of rare second-site suppressors of Drosophila RNA polymerase II mutations

  1998cites this paper
• [Cloning of cDNA for RNA polymerase subunit from the fission yeast Schizosaccharomyces pombe by heterospecific complementation in Saccharomyces cerevisiae].

  1997cites this paper
• In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin.

  1996cites this paper
• Amanitin Greatly Reduces the Rate of Transcription by RNA Polymerase II Ternary Complexes but Fails to Inhibit Some Transcript Cleavage Modes*

  1996cites this paper
• Transcriptional competition and homeosis inDrosophila

  1996cites this paper
• Characterization of the epitope recognized by a monoclonal antibody directed against the largest subunit of Drosophila RNA polymerase II.

  1995cites this paper
• Functional studies of the carboxy-terminal repeat domain of Drosophila RNA polymerase II in vivo.

  1995cites this paper
• RNAPII: a specific target for the cell cycle kinase complex.

  1994cites this paper
• Reverse genetics of Drosophila RNA polymerase II: identification and characterization of RpII140, the genomic locus for the second-largest subunit.

  1993cites this paper
• Genetics of eukaryotic RNA polymerases I, II, and III

  1993cites this paper
• Genetics of eukaryotic RNA polymerases I, II, and III.

  1993cites this paper
• Mutations in the second-largest subunit of Drosophila RNA polymerase II interact with Ubx.

  1992cites this paper
• The origin and early evolution of nucleic acid polymerases.

  1992cites this paper
• Transcription and cancer.

  1991cites this paper
• Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to alpha-amanitin.

  1990cites this paper
• Subunits shared by eukaryotic nuclear RNA polymerases.

  1990cites this paper
• Stimulation of transcription by an Ultrabithorax protein in vitro.

  1990cites this paper
• RNA polymerase B (II) and general transcription factors.

  1990cites this paper
• RNA polymerase II mutants defective in transcription of a subset of genes

  1990cites this paper
• Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery.

  1990cites this paper
• The genetics of RNA polymerases in yeasts

  1990cites this paper
• A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.

  1989cites this paper
• RNA polymerase II transcripts as targets for 5-fluorouridine cytotoxicity: antagonism of 5-fluorouridine actions by alpha-amanitin.

  1989cites this paper
• Eukaryotic RNA Polymerases

  1989cites this paper
• The evolutionary conservation of eukaryotic gene transcription

  1989cites this paper
• Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II

  1989cites this paper
• Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus

  1989cites this paper
• Eukaryotic RNA polymerases.

  1988cites this paper
• RPA190, the gene coding for the largest subunit of yeast RNA polymerase A.

  1988cites this paper
• Formation of transcription preinitiation complexes with an amanitin-resistant RNA polymerase II.

  1988cites this paper
• Transcription elongation factor SII interacts with a domain of the large subunit of human RNA polymerase II

  1988cites this paper
• The C-terminal domain of the largest subunit of RNA polymerase II of Saccharomyces cerevisiae, Drosophila melanogaster, and mammals: a conserved structure with an essential function

  1988cites this paper
• A Caenorhabditis elegans RNA polymerase II gene, ama-1 IV, and nearby essential genes.

  1988cites this paper
• Fine-structure genetics of ama-1, an essential gene encoding the amanitin-binding subunit of RNA polymerase II in Caenorhabditis elegans.

  1988cites this paper
• Transcription Elongation Factor SIIInteracts withaDomainofthe LargeSubunit ofHumanRNA Polymerase II

  1988cites this paper
• RNA polymerases B and C are more closely related to each other than to RNA polymerase A.

  1988cites this paper
• Antagonistic interactions between alleles of the RpII215 locus in Drosophila melanogaster.

  1988cites this paper
• Lethal and amanitin-resistance mutations in the Caenorhabditis elegans ama-1 and ama-2 genes.

  1988cites this paper
• Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis

  1987cites this paper
• The role of the host cell nucleus in vaccinia virus morphogenesis.

  1987cites this paper
• Localization of an alpha-amanitin resistance mutation in the gene encoding the largest subunit of mouse RNA polymerase II

  1987cites this paper
• Active site labeling of the RNA polymerases A, B, and C from yeast.

  1987cites this paper
• Cloning and sequence analysis of the mouse genomic locus encoding the largest subunit of RNA polymerase II.

  1987cites this paper
• Improved synthesis and purification of methylated amanitins using diazomethane.

  1987cites this paper
• Immunological relationships between Artemia RNA polymerases and between RNA polymerases II from different eukaryotic organisms

  1987cites this paper
• The RPO31 gene of Saccharomyces cerevisiae encodes the largest subunit of RNA polymerase III.

  1986cites this paper
• Detection of a subunit of cellular Pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions.

  1986cites this paper
• Chromosomal assignment of gene encoding the largest subunit of RNA polymerase II in the mouse

  1986cites this paper
• Molecular Cloning ofSuppressor ofSable, aDrosophila melanogaster Transposon-Mediated

  1986cites this paper
• Molecular analysis of chemically-induced mutations at the RpII215 locus of Drosophila melanogaster.

  1986cites this paper
• Identification of a nucleoside triphosphate binding site on calf thymus RNA polymerase II.

  1986cites this paper
• Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215

  1986cites this paper
• Isolation of structural genes for yeast RNA polymerases by immunological screening.

  1986cites this paper
• Influence of RNA polymerase II upon vaccinia virus-related translation examined by means of alpha-amanitin.

  1986cites this paper
• Cloning and identification of the gene coding for the 140‐kd subunit of Drosophila RNA polymerase II

  1986cites this paper
• Molecular cloning of suppressor of sable, a Drosophila melanogaster transposon-mediated suppressor

  1986cites this paper
• Transcription of SV40 and Polyoma Virus and Its Regulation

  1986cites this paper
• Eukaryotic RNA polymerases.

  1985cites this paper
• Sensitivity of Carrot Cell Cultures and RNA Polymerase II to Amatoxins : Evidence for the Inactivation of 6'-Hydroxyamatoxins.

  1985cites this paper
• Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.

  1985cites this paper
• A mutation in the largest subunit of RNA polymerase II alters RNA chain elongation in vitro.

  1985cites this paper
• Purification and immunological analysis of RNA polymerase II from Caenorhabditis elegans.

  1985cites this paper
• A selective screen for transposable element mobilization in Drosophila melanogaster.

  1985cites this paper
• Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.

  1985cites this paper
• The gene encoding the large subunit of human RNA polymerase II.

  1985cites this paper
• Structural studies on Neurospora RNA polymerases and associated proteins.

  1985cites this paper
• A unique structure at the carboxyl terminus of the largest subunit of eukaryotic RNA polymerase II.

  1985cites this paper
• Genetic and molecular variation in the RpII215 region of Drosophila melanogaster

  1985cites this paper
• Identification, molecular cloning, and mutagenesis of Saccharomyces cerevisiae RNA polymerase genes.

  1984cites this paper
• Large Subunit of Human RNA Polymerase II

  year unknowncites this paper
• Molecular Cloning and Sequencing of amai , the Gene Encoding the Largest Subunit of Caenorhabditis elegans RNA Polymerase II

  year unknowncites this paper