The External Calcium-dependent NADPH Dehydrogenase from Neurospora crassa Mitochondria*

We have inactivated the nuclear gene coding for a putative NAD(P)H dehydrogenase from the inner membrane ofNeurospora crassa mitochondria by repeat-induced point mutations. The respiratory rates of mitochondria from the resulting mutant (nde-1) were measured, using NADH or NADPH as substrates under different assay conditions. The results showed that the mutant lacks an external calcium-dependent NADPH dehydrogenase. The observation of NADH and NADPH oxidation by intact mitochondria from the nde-1 mutant suggests the existence of a second external NAD(P)H dehydrogenase. The topology of the NDE1 protein was further studied by protease accessibility, in vitro import experiments, and in silico analysis of the amino acid sequence. Taken together, it appears that most of the NDE1 protein extends into the intermembrane space in a tightly folded conformation and that it remains anchored to the inner mitochondrial membrane by an N-terminal transmembrane domain.

• Publication year

  2001

• Venue

  Journal of Biological Chemistry

• Publication date

  2001-02-09

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.M008199200PMID 11073955
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• A single external enzyme confers alternative NADH:ubiquinone oxidoreductase activity in Yarrowia lipolytica.

  1999cited by this paper
• Primary structure and characterisation of a 64 kDa NADH dehydrogenase from the inner membrane of Neurospora crassa mitochondria.

  1999cited by this paper
• Homologues of yeast and bacterial rotenone-insensitive NADH dehydrogenases in higher eukaryotes: two enzymes are present in potato mitochondria.

  1999cited by this paper
• Type 2 NADH Dehydrogenases in the CyanobacteriumSynechocystis sp. Strain PCC 6803 Are Involved in Regulation Rather Than Respiration

  1999cited by this paper
• The 24-kDa iron-sulphur subunit of complex I is required for enzyme activity.

  1999cited by this paper
• Identification of a Cytosolically Directed NADH Dehydrogenase in Mitochondria of Saccharomyces cerevisiae

  1998cited by this paper
• The Import Route of ADP/ATP Carrier into Mitochondria Separates from the General Import Pathway of Cleavable Preproteins at thetrans Side of the Outer Membrane*

  1998cited by this paper
• The Saccharomyces cerevisiae NDE1 andNDE2 Genes Encode Separate Mitochondrial NADH Dehydrogenases Catalyzing the Oxidation of Cytosolic NADH*

  1998cited by this paper
• Inactivation of the gene coding for the 30.4-kDa subunit of respiratory chain NADH dehydrogenase: is the enzyme essential for Neurospora?

  1998cited by this paper
• The role of NADP in the mitochondrial matrix

  1998cited by this paper
• Sorting of d-Lactate Dehydrogenase to the Inner Membrane of Mitochondria

  1998cited by this paper
• Protein import into mitochondria.

  1997cited by this paper
• Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa.

  1997cited by this paper
• Evidence for the presence of two rotenone-insensitive NAD(P)H dehydrogenases on the inner surface of the inner membrane of potato tuber mitochondria

  1996cited by this paper
• Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa.

  1996cited by this paper
• Internal targeting signal of the BCS1 protein: a novel mechanism of import into mitochondria.

  1996cited by this paper
• Disruption of the nuclear gene encoding the 20.8-kDa subunit of NADH:ubiquinone reductase ofNeurospora mitochondria

  1996cited by this paper
• Inactivation of genes encoding subunits of the peripheral and membrane arms of neurospora mitochondrial complex I and effects on enzyme assembly.

  1995cited by this paper
• NAD-binding domains of dehydrogenases.

  1995cited by this paper
• Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria

  1995cited by this paper
• Disruption of the gene encoding the NADH-binding subunit of NADH: ubiquinone oxidoreductase in Neurospora crassa. Formation of a partially assembled enzyme without FMN and the iron-sulphur cluster N-3.

  1994cited by this paper
• Disruption of the gene coding for the 21.3-kDa subunit of the peripheral arm of complex I from Neurospora crassa.

  1994cited by this paper
• The 12.3 kDa subunit of complex I (respiratory-chain NADH dehydrogenase) from Neurospora crassa: cDNA cloning and chromosomal mapping of the gene.

  1993cited by this paper
• Functional independence of the protein translocation machineries in mitochondrial outer and inner membranes: passage of preproteins through the intermembrane space.

  1993cited by this paper
• Import of cytochrome c heme lyase into mitochondria: a novel pathway into the intermembrane space.

  1992cited by this paper
• Sexual development genes of Neurospora crassa.

  1992cited by this paper
• Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae.

  1991cited by this paper
• Premeiotic instability of repeated sequences in Neurospora crassa.

  1990cited by this paper
• Molecular cloning of subunits of complex I from Neurospora crassa. Primary structure and in vitro expression of a 22-kDa polypeptide.

  1990cited by this paper
• Nucleotide sequence of a full-length cDNA coding for the mitochondrial precursor protein of the beta-subunit of F1-ATPase from Neurospora crassa.

  1990cited by this paper
• Molecular cloning of a Neurospora crassa carotenoid biosynthetic gene (albino-3) regulated by blue light and the products of the white collar genes

  1989cited by this paper
• MOM19, an import receptor for mitochondrial precursor proteins.

  1989cited by this paper
• Assembly kinetics and identification of precursor proteins of complex I from Neurospora crassa.

  1989cited by this paper
• A rapid, high yield mini-prep method for isolation of total genomic DNA from fungi.

  1988cited by this paper
• DNA sequence duplications trigger gene inactivation in Neurospora crassa.

  1988cited by this paper
• Mitochondrial protein import: identification of processing peptidase and of PEP, a processing enhancing protein.

  1988cited by this paper
• [41] Isolation of submitochondrial particles with different polarities

  1987cited by this paper
• The mitochondrial respiratory chain of yeast. Structure and biosynthesis and the role in cellular metabolism.

  1987cited by this paper
• Efficient cloning of genes of Neurospora crassa.

  1986cited by this paper
• The regulation of exogenous NAD(P)H oxidation in spinach (Spinacia oleracea) leaf mitochondria by pH and cations.

  1985cited by this paper
• The mitochondrial electron transport and oxidative phosphorylation system.

  1985cited by this paper
• Rotenone- and Cyanide-insensitive Respiration in Mitochondria from Neurospora crassa

  1983cited by this paper
• Binding and screening by cations and the effect on exogenous NAD(P)H oxidation in Neurospora crassa mitochondria.

  1982cited by this paper
• Direct evidence for the presence of a rotenone‐resistant NADH dehydrogenase on the inner surface of the inner membrane of plant mitochondria

  1982cited by this paper
• A specific role for Ca2+ in the oxidation of exogenous NADH by Jerusalem-artichoke (Helianthus tuberosus) mitochondria.

  1981cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Preparation of polypeptide subunits of cytochrome oxidase from Neurospora crassa.

  1977cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• An efficient mRNA-dependent translation system from reticulocyte lysates.

  1976cited by this paper
• Characterization of Neurospora crassa mitochondria prepared with a grind-mill.

  1970cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• [4] Genetic and microbiological research techniques for Neurospora crassa

  1970cited by this paper

• Analysis of the Respiratory Activity in the Antarctic Yeast Rhodotorula mucilaginosa M94C9 Reveals the Presence of Respiratory Supercomplexes and Alternative Elements

  2024cites this paper
• Transcriptome profiling of barley and tomato shoot and root meristems unravels physiological variations underlying photoperiodic sensitivity

  2022cites this paper
• Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of Ogataea parapolymorpha

  2021influential citation
• Leishmania type II dehydrogenase is essential for parasite viability irrespective of the presence of an active complex I

  2021cites this paper
• Biological Evaluation and Mechanistic Studies of Quinolin-(1H)-Imines as a New Chemotype against Leishmaniasis

  2021cites this paper
• Pan‐Genome‐Scale Network Reconstruction: Harnessing Phylogenomics Increases the Quantity and Quality of Metabolic Models

  2020cites this paper
• Respiratory Chain and Energy Metabolism of Corynebacterium glutamicum

  2020cites this paper
• Alternative Type II NAD(P)H Dehydrogenases in the Mitochondria of Protists and Fungi.

  2019cites this paper
• Expression of alternative NADH dehydrogenases (NDH‐2) in the phytopathogenic fungus Ustilago maydis

  2018cites this paper
• Pan-genome-scale network reconstruction: a framework to increase the quantity and quality of metabolic network reconstructions throughout the tree of life

  2018cites this paper
• Structural and Functional insights into the catalytic mechanism of the Type II NADH:quinone oxidoreductase family

  2017cites this paper
• Studies on calcium signaling genes phospholipase C-1, secretory phospholipase A2, and calcium proton exchanger-1 in Neurospora crassa

  2017cites this paper
• and insights into the catalytic mechanism of the Type II NADH:quinone oxidoreductase

  2017cites this paper
• The evolution of substrate specificity-associated residues and Ca(2+) -binding motifs in EF-hand-containing type II NAD(P)H dehydrogenases.

  2016cites this paper
• Type II NADH:quinone oxidoreductase family: phylogenetic distribution, structural diversity and evolutionary divergences.

  2016cites this paper
• Supramolecular organization of bacterial aerobic respiratory chains: From cells and back.

  2016cites this paper
• Mitochondrial type II NAD(P)H dehydrogenases in fungal cell death

  2015influential citation
• Unravelling New Metabolic Pathways: Supramolecular Organization of Aerobic Bacteria Respiratory Chains

  2015cites this paper
• Involvement of mitochondrial proteins in calcium signaling and cell death induced by staurosporine in Neurospora crassa.

  2015cites this paper
• Structural and functional investigation of type II NADH:quinone oxidoreductase from the pathogenic bacteria Vibrio cholerae and Staphylococcus aureus

  2015cites this paper
• Study of alternative NADH dehydrogenase and mitochondrial fumarate reductase in Leishmania Infantum

  2014cites this paper
• External NAD(P)H dehydrogenases in Acanthamoeba castellanii mitochondria.

  2014cites this paper
• The molecular basis of programmed cell death in Neurospora crassa

  2014cites this paper
• Corynebacterium glutamicum

  2013cites this paper
• Novel Insights into the Role of Neurospora crassa NDUFAF2, an Evolutionarily Conserved Mitochondrial Complex I Assembly Factor

  2013cites this paper
• Characterization of Apoptosis-Related Oxidoreductases from Neurospora crassa

  2012influential citation
• Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa.

  2012cites this paper
• Disruption of alternative NAD(P)H dehydrogenases leads to decreased mitochondrial ROS in Neurospora crassa.

  2012cites this paper
• The alternative NADH dehydrogenase is present in mitochondria of some animal taxa.

  2011cites this paper
• Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1

  2011cites this paper
• Regulation of respiration in plants: a role for alternative metabolic pathways.

  2011cites this paper
• The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance.

  2010cites this paper
• Mitochondria and Respiration

  2010cites this paper
• ALTERED EXPRESSION OF THE NON-PHOSPHORYLATION ELECTRON TRANSPORT CHAIN EFFECTS GROWTH AND STRESS TOLERANCE IN ARABIDOPSIS THALIANA

  2010influential citation
• Characterization of Nda2, a Plastoquinone-reducing Type II NAD(P)H Dehydrogenase in Chlamydomonas Chloroplasts*

  2009cites this paper
• Chapter 6 Reactive Oxygen Species in Phanerochaete chrysosporium Relationship Between Extracellular Oxidative and Intracellular Antioxidant Systems

  2009cites this paper
• Type II NADH dehydrogenase of the respiratory chain of Plasmodium falciparum and its inhibitors.

  2009cites this paper
• A Redox-Mediated Modulation of Stem Bolting in Transgenic Nicotiana sylvestris Differentially Expressing the External Mitochondrial NADPH Dehydrogenase1[W][OA]

  2009cites this paper
• Effects of mitochondrial complex III disruption in the respiratory chain of Neurospora crassa

  2009cites this paper
• The multiplicity of dehydrogenases in the electron transport chain of plant mitochondria.

  2008cites this paper
• Mitochondrial function in the yeast form of the pathogenic fungus Paracoccidioides brasiliensis

  2008cites this paper
• Physiological analysis of cyanide-insensitive alternative oxidase and its application to metabolic engineering of citric acid-producing Aspergillus niger

  2008cites this paper
• Effects ofN,N-bis(3-aminopropyl)dodecylamine on antioxidant enzyme activities, mitochondrial morphology and metabolism inAspergillus niger

  2008cites this paper
• The three families of respiratory NADH dehydrogenases.

  2008cites this paper
• Transcriptomics and quantitative physiology of ß-lactam producing Penicillium chrysogenum

  2008influential citation
• Mitochondrial transporters as novel targets for intracellular calcium signaling.

  2007cites this paper
• Ca2+-binding and Ca2+-independent Respiratory NADH and NADPH Dehydrogenases of Arabidopsis thaliana*

  2007cites this paper
• Modification of substrate specificity in single point mutants of Agrobacterium tumefaciens type II NADH dehydrogenase

  2007cites this paper
• The external alternative NAD(P)H dehydrogenase NDE3 is localized both in the mitochondria and in the cytoplasm of Neurospora crassa.

  2007cites this paper
• Mitochondrial Electron Transport and Oxidative Stress

  2007cites this paper
• Mitochondrial free radical generation and lifespan control in the fungal aging model Podospora anserina.

  2006cites this paper
• Reoxidation of cytosolic NADPH in Kluyveromyces lactis.

  2006cites this paper
• Enzymic analysis of NADPH metabolism in beta-lactam-producing Penicillium chrysogenum: presence of a mitochondrial NADPH dehydrogenase.

  2006cites this paper
• Substrate‐inducible versions of internal alternative NADH : ubiquinone oxidoreductase from Yarrowia lipolytica

  2006cites this paper
• Characterization of the second external alternative dehydrogenase from mitochondria of the respiratory yeast Kluyveromyces lactis.

  2006cites this paper
• Effects of N,N-bis(3-aminopropyl)dodecylamine on antioxidant enzyme activities, mitochondrial morphology and metabolism in Aspergillus niger.

  2006cites this paper
• Neurospora Strains Harboring Mitochondrial Disease-Associated Mutations in Iron-Sulfur Subunits of Complex I

  2005cites this paper
• The nuclear genes encoding the internal (KlNDI1) and external (KlNDE1) alternative NAD(P)H:ubiquinone oxidoreductases of mitochondria from Kluyveromyces lactis.

  2005cites this paper
• Electron Transfer Ability from NADH to Menaquinone and from NADPH to Oxygen of Type II NADH Dehydrogenase of Corynebacterium glutamicum

  2005cites this paper
• Composition of complex I from Neurospora crassa and disruption of two "accessory" subunits.

  2005cites this paper
• HDQ (1-Hydroxy-2-dodecyl-4(1H)quinolone), a High Affinity Inhibitor for Mitochondrial Alternative NADH Dehydrogenase

  2005cites this paper
• Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze

  2004cites this paper
• The main external alternative NAD(P)H dehydrogenase of Neurospora crassa mitochondria.

  2004influential citation
• New Insights into Type II NAD(P)H:Quinone Oxidoreductases

  2004influential citation
• Alternative NAD(P)H dehydrogenases of plant mitochondria.

  2004cites this paper
• Alternative mitochondrial electron transport proteins in the higher plants

  2004cites this paper
• Respiratory Chains from Aerobic Thermophilic Prokaryotes

  2004cites this paper
• Identification of a mitochondrial external NADPH dehydrogenase by overexpression in transgenic Nicotiana sylvestris.

  2004cites this paper
• Arabidopsis Genes Encoding Mitochondrial Type II NAD(P)H Dehydrogenases Have Different Evolutionary Origin and Show Distinct Responses to Light1

  2003cites this paper
• The 9.8 kDa subunit of complex I, related to bacterial Na(+)-translocating NADH dehydrogenases, is required for enzyme assembly and function in Neurospora crassa.

  2003cites this paper
• The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria.

  2003influential citation
• Identification of AtNDI1, an Internal Non-Phosphorylating NAD(P)H Dehydrogenase in Arabidopsis Mitochondria1

  2003influential citation
• From NADH to ubiquinone in Neurospora mitochondria.

  2002cites this paper
• Two mechanisms for oxidation of cytosolic NADPH by Kluyveromyces lactis mitochondria

  2002cites this paper
• A new dawn for plant mitochondrial NAD(P)H dehydrogenases.

  2002cites this paper
• On Complex I and Other NADH:Ubiquinone Reductases of Neurospora crassa Mitochondria

  2001cites this paper
• NADPH dehydrogenase 4 Membrane association and immunodetection of alternative NAD(P)H dehydrogenases in mitochondria 4.1 Import and membrane association of internal NAD(P)H dehydrogenases 4.2 Immunodetection of the internal rotenone-insensitive NADH dehydrogenase

  2001cites this paper
• Pyridine Nucleotides in Plant Mitochondria. Amounts,metabolism and contribution to electron transport

  2001cites this paper
• PLANT MITOCHONDRIA AND OXIDATIVE STRESS: Electron Transport, NADPH Turnover, and Metabolism of Reactive Oxygen Species.

  2001cites this paper
• Rotenone-insensitive NAD(P)H dehydrogenases in plants: Immunodetection and distribution of native proteins in mitochondria.

  2001cites this paper