Mouse serum amyloid A protein (SAA5) structure and expression.

A novel member of the mouse serum amyloid A protein family, SAA5, has been identified as a normal apolipoprotein component of non-acute-phase high density lipoprotein (HDL). The structure of SAA5 was derived from a clone isolated from a normal Balb/c liver cDNA library. The clone predicts a pre-SAA5 molecule of 130 residues from which an 18-residue leader peptide is cleaved. The mature molecule has an octapeptide insert spanning from position 70 to 77. Similar inserts are found in human C-SAA and, paradoxically, in acute-phase SAA molecules of a number of other species. There is 48% amino acid identity between apo-SAA5 and the other mouse SAA proteins and 57% identity between the human C-SAA and apo-SAA5. The SAA5 mRNA is three times larger than previously identified SAA mRNAs. Although SAA5 is constitutively expressed in the liver, it has a rapid albeit muted response to inflammatory stimuli. The increase of SAA5 mRNA is due to increased transcription rather than mRNA stabilization. Plasma SAA5 levels during the acute phase are biphasic, either because of translational control or displacement from HDL and rapid clearance. We propose that constitutive SAAs (SAA5) on normal HDL contribute to its normal physiological role, whereas the dramatically inducible family members (SAA1, SAA2, SAA3) equip this particle for an altered functional role during inflammation.

• Publication year

  1994

• Venue

  Journal of Biological Chemistry

• Publication date

  1994-02-11

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(17)41827-8PMID 8308037
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Plasma SAA5 levels during the acute phase are biphasic, consistent with translational control or redistribution from HDL followed by rapid clearance.

  Confidence 0.90

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• SAA5 mRNA is constitutively expressed in liver, responds rapidly but only modestly to inflammatory stimuli, and its induction reflects increased transcription rather than mRNA stabilization.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• The cloned mouse SAA5 sequence predicts a 130-residue pre-SAA5 with an 18-residue leader peptide and a mature protein containing an octapeptide insert.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• SAA5 is identified as a normal apolipoprotein component of non-acute-phase HDL.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

• acute-phase plasma saa5

  measurement

  The circulating SAA5 protein pool measured during the acute-phase response.

  Aliases: plasma SAA5 levels during the acute phase

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• balb/c liver cdna library

  resource

  A cDNA library prepared from normal Balb/c mouse liver and used as the source of the SAA5 clone.

  Aliases: normal Balb/c liver cDNA library

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• inflammatory stimuli

  stimulus

  Inflammation-associated signals used to assess the inducibility of SAA5 expression in liver.

  Aliases: inflammatory stimulus

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• non-acute-phase hdl

  lipoprotein

  The circulating high-density lipoprotein fraction present under non-inflammatory conditions and discussed here as the carrier of SAA5.

  Aliases: normal HDL

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• octapeptide insert

  sequence feature

  An eight-amino-acid insertion within the mature SAA5 protein sequence spanning residues 70 to 77.

  Aliases: eight-residue insert

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• saa5

  protein

  A mouse serum amyloid A family apolipoprotein characterized in this work as a liver-derived HDL-associated protein.

  Aliases: apo-SAA5

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• transcriptional upregulation

  process

  An increase in mRNA production caused by higher transcriptional activity rather than enhanced message stability.

  Aliases: increased transcription

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

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