Optimizing transfection of primary human umbilical vein endothelial cells using commercially available chemical transfection reagents.

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Journal of Biomolecular Techniques

• Publication date

  2010-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  PMID 20592869PMCID PMC2884313
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transfection efficiency of cationic lipids with different hydrophobic domains in gene delivery.

  2010cited by this paper
• Physical methods of nucleic acid transfer: general concepts and applications

  2009cited by this paper
• RGS5, a Hypoxia-inducible Apoptotic Stimulator in Endothelial Cells*

  2009cited by this paper
• Non-viral gene delivery with cationic liposome-DNA complexes.

  2008cited by this paper
• Progress in developing cationic vectors for non-viral systemic gene therapy against cancer.

  2008cited by this paper
• Modification of adenovirus gene transfer vectors with synthetic polymers: a scientific review and technical guide.

  2008cited by this paper
• Identification of replicative senescence-associated genes in human umbilical vein endothelial cells by an annealing control primer system.

  2008cited by this paper
• Improving safety of gene therapy.

  2008cited by this paper
• Nonviral approaches for targeted delivery of plasmid DNA and oligonucleotide.

  2008cited by this paper
• Toward Development of Artificial Viruses for Gene Therapy: A Comparative Evaluation of Viral and Non‐viral Transfection

  2008cited by this paper
• Angiogenic profiling and comparison of immortalized endothelial cells for functional genomics.

  2008cited by this paper
• Endothelial cell COX-2 expression and activity in hypoxia.

  2006cited by this paper
• Kinase domain insert containing receptor promoter controlled suicide gene system selectively kills human umbilical vein endothelial cells.

  2005cited by this paper
• Polymers for DNA Delivery

  2005cited by this paper
• Regulatory Considerations for Novel Gene Therapy Products: A Review of the Process Leading to the First Clinical Lentiviral Vector

  2005cited by this paper
• Differential Ability of Human Endothelial Cells to Internalize and Express Exogenous DNA

  2004cited by this paper
• Transductional and transcriptional targeting of adenovirus for clinical applications.

  2004cited by this paper
• Current developments in gene transfection agents.

  2004cited by this paper
• Dual targeting of gene delivery by genetic modification of adenovirus serotype 5 fibers and cell-selective transcriptional control

  2004cited by this paper
• Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells.

  2002cited by this paper
• DNA nanoparticles and development of DNA delivery vehicles for gene therapy.

  2002cited by this paper
• Cancer gene therapy: 'delivery, delivery, delivery' .

  2002cited by this paper
• Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation.

  2002cited by this paper
• Green fluorescent protein as a novel tool to measure apoptosis and necrosis.

  2001cited by this paper
• Inhibition of Hydrophobic Protein-MediatedCandida albicans Attachment to Endothelial Cells during Physiologic Shear Flow

  2001cited by this paper
• Highly efficient transduction of endothelial cells by targeted artificial virus-like particles

  2001cited by this paper
• Fluorescence produced by transfection reagents can be confused with green fluorescent proteins in mammalian cells.

  2001cited by this paper
• Cationic liposomes induce macrophage apoptosis through mitochondrial pathway.

  2001cited by this paper
• Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody.

  2000cited by this paper
• Transfer and expression of foreign genes in mammalian cells.

  2000cited by this paper
• High‐efficiency transient transfection of endothelial cells for functional analysis

  2000cited by this paper
• Endothelial cell transfection with cationic liposomes and herpes simplex-thymidine kinase mediated killing

  1998cited by this paper
• Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of the nuclear membrane.

  1997cited by this paper
• Optimization of transfection of human endothelial cells.

  1997cited by this paper
• Reporter gene expression for monitoring gene transfer.

  1997cited by this paper
• Towards endothelial-cell-directed cancer immunotherapy: in vitro expression of human recombinant cytokine genes by human and mouse primary endothelial cells.

  1996cited by this paper
• Efficient gene transfer into human umbilical vein endothelial cells allows functional analysis of the human tissue factor gene promoter

  1994cited by this paper
• Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.

  1973cited by this paper

• Manipulating PARK7/DJ-1 Levels by Genotoxic Stress Alters Noncoding RNAs and Cellular Homeostasis

  2025cites this paper
• Targeting lysozyme 2 in endocardium promotes rapid recovery by modulating remote injury signals.

  2025cites this paper
• STING inhibition enables efficient plasmid-based gene expression in primary vascular cells: A simple and cost-effective transfection protocol

  2024cites this paper
• Knockdown of DJ-1 Resulted in a Coordinated Activation of the Innate Immune Antiviral Response in HEK293 Cell Line

  2024cites this paper
• Engineered vitamin E-tethered non-immunogenic facial lipopeptide for developing improved siRNA based combination therapy against metastatic breast cancer

  2023cites this paper
• Selectively Fluorinated PAMAM–Arginine Conjugates as Gene Delivery Vectors

  2023cites this paper
• Gene silencing of endothelial von Willebrand factor reduces the susceptibility of human endothelial cells to SARS‐CoV‐2 infection

  2023cites this paper
• TRAF7 is an essential regulator of blood vessel integrity during mouse embryonic and neonatal development

  2023influential citation
• In situ transient transfection of 3D cell cultures and tissues, a promising tool for tissue engineering and gene therapy.

  2023cites this paper
• HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes

  2022cites this paper
• Intracellular and Extracellular Antifreeze Protein Significantly Improves Mammalian Cell Cryopreservation

  2022cites this paper
• SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells

  2021cites this paper
• Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells

  2021cites this paper
• Transfection types, methods and strategies: a technical review

  2021influential citation
• Antisense Oligonucleotide-Mediated Splice Switching: Potential Therapeutic Approach for Cancer Mitigation

  2021cites this paper
• Smart arginine-equipped polycationic nanoparticles for p/CRISPR delivery into cells

  2021cites this paper
• Acid-Sensitive Surfactants Enhance the Delivery of Nucleic Acids.

  2021cites this paper
• Plasma polymerized nanoparticles effectively deliver dual siRNA and drug therapy in vivo

  2020cites this paper
• New-Generation Benzimidazole-Based Plasmid Delivery Reagents with High Transfection Efficiencies on the Mammalian Cells

  2020cites this paper
• Cell-cell interaction in a co-culture system consisting of CRISPR/Cas9 mediated GFP knock- in HUVECs and MG-63 cells in alginate-GelMA based nanocomposites hydrogel as a 3D scaffold.

  2020cites this paper
• Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice

  2020cites this paper
• Investigating the Role of the 3’UTR of Claudin-5 and Circadian Rhythms at Tight Junctions in the Blood-Brain Barrier A thesis submitted to The University of Dublin for the degree of Master of Science 2020

  2020influential citation
• Biphasic Gene Electrotransfer Enhances Gene Delivery In Vitro

  2020cites this paper
• Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus

  2019cites this paper
• Delivery of RALA/siFKBPL Nanoparticles via Electrospun Bilayer Nanofibres: an innovative angiogenic therapy for wound repair.

  2019cites this paper
• Fundamentals of Vascular Biology

  2019cites this paper
• Topoisomerase I is required for the expression of long genes in glial cells and is dysregulated in ASD brain

  2019cites this paper
• Helium-mediated regulation of endothelial permeability: involvement of Caveolin-1, VE-Cadherin and Connexin-43

  2019cites this paper
• Endothelial Cell Isolation and Manipulation

  2019cites this paper
• Curcumin exerts anti-inflammatory and vasoprotective effects through amelioration of NFAT-dependent endothelin-1 production in mice with acute Chagas cardiomyopathy

  2018cites this paper
• Effect of the development of a cell barrier on nanoparticle uptake in endothelial cells.

  2018cites this paper
• Ultrastructural Analysis of Vesicular Transport in Electrotransfection

  2018cites this paper
• Mécanismes impliqués dans la modulation de la néovascularisation post-ischémique : rôle de la rénine et des microARNs

  2018cites this paper
• Profiling and exploiting lipid-based nanoparticles in vitro and in vivo

  2018cites this paper
• Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents.

  2018cites this paper
• Leukocytes and drug-resistant cancer cells are targets for intracellular delivery by adenoviral dodecahedron.

  2018cites this paper
• Endothelial Cell Focal Adhesion Regulates Transendothelial Migration and Subendothelial Crawling of T Cells

  2018cites this paper
• Expression of c-FLIP in a rat model of sepsis and its effects on endothelial apoptosis

  2017cites this paper
• The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome

  2017cites this paper
• Evolution and epigenetic control of acute inflammation in mammalian endothelial cells

  2017cites this paper
• Overexpression of Epidermal growth factor like domain 7 using an inducible PiggyBac vector

  2017cites this paper
• Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells.

  2017cites this paper
• The Use of Single Wall Carbon Nanotubes as a Delivery System for siRNA

  2016cites this paper
• Optimizaton of Cationic Lipid Mediated Transfection of pEGFP-c1 and pJ- EPO Plasmids in Chinese Hamster Ovary (CHO) Cells Attached Culture for Transient and Stable Recombinant Human Erythropoietin (rhEPO) Expression

  2016cites this paper
• Non-viral magnetic engineering of endothelial cells with microRNA and plasmid-DNA-An optimized targeting approach.

  2016cites this paper
• Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

  2016cites this paper
• Study the function of the newly discovered TrkB receptor fragment (TrkB-ICD) formed by calpain cleavage

  2016cites this paper
• Cyclic AMP responsive element-binding protein promotes renal cell carcinoma proliferation probably via the expression of spindle and kinetochore-associated protein 2

  2016cites this paper
• CD147 overexpression promotes tumorigenicity in Chinese hamster ovary cells

  2016cites this paper
• Overexpression of LOXIN Protects Endothelial Progenitor Cells From Apoptosis Induced by Oxidized Low Density Lipoprotein

  2016cites this paper
• Different Influences of Lipofection and Electrotransfection on In Vitro Gene Delivery to Primary Cultured Cortex Neurons.

  2016cites this paper
• Cationic β-Cyclodextrin–Chitosan Conjugates as Potential Carrier for pmCherry-C1 Gene Delivery

  2016cites this paper
• Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery

  2015cites this paper
• Endothelial cell-targeted pVEGF165 polyplex plays a pivotal role in inhibiting intimal thickening after vascular injury

  2015cites this paper
• Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes

  2015cites this paper
• Intermittent High Glucose Implements Stress-Induced Senescence in Human Vascular Endothelial Cells: Role of Superoxide Production by NADPH Oxidase

  2015cites this paper
• Exploring the role of transmembrane 4 L six family member 1 (Tm4sf1) in the control of tip cell behaviour during sprouting angiogenesis

  2015cites this paper
• Characterizing angiodysplasia in type 2 von Willebrand disease (VWD) using blood outgrowth endothelial cells (BOECs)

  2015cites this paper
• Therapeutic Effect of Human Adipose‐Derived Stromal Cells Cluster in Rat Hind‐Limb Ischemia

  2014cites this paper
• A high content assay to assess cellular fitness.

  2014cites this paper
• Microcapsule internalization by cells in vitro caused by physical and biochemical stimuli

  2014cites this paper
• Characterization of gemini nanoparticle assembly by fluorescence correlation spectroscopy

  2014cites this paper
• Evaluating the role of low-speed centrifugation towards transfecting human peripheral blood mononuclear cell culture.

  2014cites this paper
• A Study of Effects on MMP14 Transcriptional Regulation and Angiogenesis by Hypoxia and Statins

  2014cites this paper
• Unraveling the effects of siRNA carrier systems on cell physiology: a multiparametric approach demonstrated on dextran nanogels.

  2014cites this paper
• Electrotransfection and Lipofection Show Comparable Efficiency for In Vitro Gene Delivery of Primary Human Myoblasts

  2014cites this paper
• Studies on the effect of interleukin-33 on gene expression and lipid composition of macrophages

  2014cites this paper
• Isolation, cultivation and transfection of human keratinocytes

  2014cites this paper
• Nucleofection-Mediated α1,3-galactosyltransferase Gene Inactivation and Membrane Cofactor Protein Expression for Pig-to-Primate Xenotransplantation

  2013cites this paper
• Engineered CHO cell line with coxsackie and adenovirus gene enhance the susceptibility of adenovirus infection

  2013cites this paper
• Int6/eIF3e silenced HIF2α stabilization enhances migration and tube formation of HUVECs via IL-6 and IL-8 signaling.

  2013cites this paper
• AP-2α suppresses invasion in BeWo cells by repression of matrix metalloproteinase-2 and -9 and up-regulation of E-cadherin

  2013cites this paper
• Characterisation of enzyme prodrug gene therapy combinations in coated spheroids and vascular networks in vitro

  2012cites this paper
• SELECTION OF pEGFP-c 1-TRANSFECTED-CHOK 1 CELLS BY G 418 DECREASED THE EXPRESSION OF GREEN FLUORESCENT PROTEIN

  2012cites this paper
• siRNA Delivery: From Lipids to Cell‐penetrating Peptides and Their Mimics

  2012cites this paper
• Cell-specific multifunctional processing of heterogeneous cell systems in a single laser pulse treatment.

  2012cites this paper
• Development and Characterization of a Controlled Expression System for Osteogenic Genes

  2011cites this paper
• Desensitisation of the Pituitary Vasopressin Receptor: Development and Use of a Stably-Transfected Model Cell System to Assess the Role of G Protein-Coupled Receptor Kinases

  2011cites this paper
• Selective gene transfection of individual cells in vitro with plasmonic nanobubbles.

  2011cites this paper
• Gene Therapy and Cystic Fibrosis Melisa

  year unknowncites this paper