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Effect of Benomyl on Chitinase and -1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum

M. S. El-Katatny,H. El-Komy,G. M. Shaban,A. Hetta,Momein H. A. El-Katatny

Published 2004 in Unknown venue

ABSTRACT

Summary On PDA-benomyl plates growth of Trichoderma harzianum was inhibited by 20 and 30 % at benomyl 1 and 2 g/mL, respectively, and was completely inhibited at 5 g/mL. In minimal synthetic medium (MSM) amended with different concentrations of benomyl (1.0, 3.0, 5.0, 7.0 and 10.0 g/mL), fungal immobilisation improved chitinase and -1,3-glucanase production at low benomyl concentrations (1, 3 and 5 g/mL). Further increase in the production of both enzymes was obtained by immobilisation at higher benomyl concentrations (7 and 10 g/mL). Fungal immobilisation increased bound chitinase by 15- to 30-fold at 3 and 5 g/mL benomyl concentration, respectively. However, no effect was obtained on the bound -1,3-glucanase. Different benomyl concentrations (0.3 to 1500 g/mL) had no significant inhibitory effect on the activities of free or immobilised chitinase and -1,3-glucanase. It could be suggested that either immobilised Trichoderma or immobilised chitinase and -1,3-glucanase could be used in combination with benomyl to control plant pathogens.

PUBLICATION RECORD

  • Publication year

    2004

  • Venue

    Unknown venue

  • Publication date

    Unknown publication date

  • Fields of study

    Biology, Materials Science, Environmental Science

  • Identifiers

    No identifiers available.

  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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