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ACCUMULATION OF CITRATE AND MALATE BY MITOCHONDRIA.

J. Gamble

Published 1965 in Journal of Biological Chemistry

ABSTRACT

Previous studies have established that potassium is retained by mitochondria in relatively fixed amounts through washing procedures with sucrose (0.25 M) or sodium chloride (0.15 M) (l-3). The potassium will exchange with free potassium in the surrounding fluids, but it will not exchange with or be dis-placed by sodium. This type of retention differs from that of intact cells in which the concentrations of ions on the inside are in part a function of the concentrations outside and in which loss of potassium is associated usually with a gain of sodium. The mitochondria retain organic and inorganic phosphate in quantities that also are fixed and that are sufficient to supply anionic equivalence for the potassium (4, 5). In the present study, the experiments have shown a rapid accumulation of citrate and malate by isolated mitochondria. Once accumulated, these materials too are maintained at relatively fixed concentrations and are not removed by washing with isotonic sucrose. The accumulation of these anions does not depend on active respiration but is inactivated by dinitrophenol. Relationships between the retention of these substances and the retention of potassium and phosphate are described. obtained

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