Phosphorylation by actin kinase of the pointed end domain on the actin molecule.

Fragmin from plasmodium of Physarum polycephalum binds G-actin and severs F-actin in the presence of Ca2+ over 10(-6) M. The fragmin-actin complex consisting of fragmin and G-actin nucleates actin polymerization and caps the barbed (fast growing) end of F-actin, regardless of the concentrations of Ca2+, and the actin filaments are shortened. Actin kinase purified from plasmodium abolishes the nucleation and capping activities of the complex by phosphorylating actin of the fragmin-actin complex (Furuhashi, K., and Hatano, S. (1990) J. Cell. Biol. 111, 1081-1087). This inactivation of the complex leads to production of long actin filaments. We obtained evidence that Physarum actin is phosphorylated by actin kinase at Thr-201, and probably at Thr-202 and/or Thr-203, with 1 mol of phosphate distributed among them. This finding raises the possibility that the site of phosphorylation, Thr-201 to Thr-203, is positioned on the pointed (slow growing) end domain of the actin molecule, because growth of actin filaments from the fragmin-actin complex occurs only from the pointed end. These observations are consistent with a model of the three-dimensional structure of G-actin. Inactivation of the fragmen-actin complex may follow phosphorylation of the pointed end domain of actin.

• Publication year

  1992

• Venue

  Journal of Biological Chemistry

• Publication date

  1992-05-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(19)50427-6PMID 1315751
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Primary structure of profilins from two species of Echinoidea and Physarum polycephalum.

  1990cited by this paper
• Atomic model of the actin filament

  1990cited by this paper
• Control of actin filament length by phosphorylation of fragmin-actin complex

  1990cited by this paper
• Phosphorylation sites linked to glial filament disassembly in vitro locate in a non-alpha-helical head domain.

  1990cited by this paper
• Atomic structure of the actin: DNase I complex

  1990cited by this paper
• A fragmin-like protein from plasmodium of Physarum polycephalum that severs F-actin and caps the barbed end of F-actin in a Ca2+-sensitive way.

  1989cited by this paper
• Polyphosphoinositide micelles and polyphosphoinositide-containing vesicles dissociate endogenous gelsolin-actin complexes and promote actin assembly from the fast-growing end of actin filaments blocked by gelsolin.

  1987cited by this paper
• Microinjection of gelsolin into living cells

  1987cited by this paper
• Actin-fragmin interactions as revealed by chemical cross-linking.

  1986cited by this paper
• Liver isozyme of rabbit glycogen synthase. Amino acid sequences surrounding phosphorylation sites recognized by cyclic AMP-dependent protein kinase.

  1986cited by this paper
• Actin and actin-binding proteins. A critical evaluation of mechanisms and functions.

  1986cited by this paper
• Phosphorylation of Amoeba G-actin and its effect on actin polymerization.

  1986cited by this paper
• Organization of the actin multigene family of Dictyostelium discoideum and analysis of variability in the protein coding regions.

  1985cited by this paper
• Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin

  1985cited by this paper
• F-actin is intermolecularly crosslinked by N,N'-p-phenylenedimaleimide through lysine-191 and cysteine-374.

  1984cited by this paper
• Amino acid sequence of Acanthamoeba actin.

  1984cited by this paper
• Rapid analysis of amino acids using pre-column derivatization.

  1984cited by this paper
• Actin genes and actin messenger RNA in Acanthamoeba castellanii. Nucleotide sequence of the split actin gene I.

  1982cited by this paper
• Effect of fragmin on actin polymerization: evidence for enhancement of nucleation and capping of the barbed end.

  1982cited by this paper
• Actin polymerization and its regulation by proteins from nonmuscle cells.

  1982cited by this paper
• Complete nucleotide sequence of a sea urchin actin gene

  1982cited by this paper
• Actin-binding proteins—regulators of cell architecture and motility

  1982cited by this paper
• Fragmin: a calcium ion sensitive regulatory factor on the formation of actin filaments.

  1980cited by this paper
• Transforming gene product of Rous sarcoma virus phosphorylates tyrosine

  1980cited by this paper
• Vegetative Dictyostelium cells containing 17 actin genes express a single major actin

  1980cited by this paper
• The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.

  1979cited by this paper
• Control of cytoplasmic actin gel–sol transformation by gelsolin, a calcium-dependent regulatory protein

  1979cited by this paper
• The amino acid sequence of Physarum actin

  1978cited by this paper
• A simple method for the isolation of actin from myxomycete plasmodia.

  1977cited by this paper
• The primary structure of actin from rabbit skeletal muscle. Completion and analysis of the amino acid sequence.

  1975cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• The O-GlcNAcylation of β-actin Ser199 controls nuclear speckle localization and is dysregulated in diabetes

  2025cites this paper
• Actin.

  2020cites this paper
• O-GlcNAcylation and Phosphorylation of β-Actin Serine199 in Diabetic Nephropathy.

  2019cites this paper
• Cations Stiffen Actin Filaments by Adhering a Key Structural Element to Adjacent Subunits

  2016cites this paper
• Post-translational modification-regulated leukocyte adhesion and migration

  2016cites this paper
• The physiological role of polycomb group protein Ezh2 in dendritic cells

  2016cites this paper
• Regulation of actin by ion-linked equilibria.

  2013cites this paper
• The nature of the globular- to fibrous-actin transition

  2009cites this paper
• Thenature oftheglobular-tofibrous-actin

  2009cites this paper
• Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum.

  2006cites this paper
• Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process*

  2003cites this paper
• The microglial response to energy manipulation and glutamate : implications for neuronal survival in ischaemia.

  2002cites this paper
• Selected BTB/POZ‐kelch proteins bind ATP

  2002cites this paper
• Identification and characterization of a cathepsin B-like protease in Physarum sclerotium.

  2002cites this paper
• Involvement of Actin Dephosphorylation in Germination of Physarum Sclerotium

  2002cites this paper
• Calcium Inhibition of Cytoplasmic Streaming

  2000cites this paper
• A novel endogenous PP2C-like phosphatase dephosphorylates casein kinase II-phosphorylated Physarum fragmin.

  2000cites this paper
• Calcium regulation of the actin-myosin interaction of Physarum polycephalum.

  1999cites this paper
• Protein phosphatase 2A inhibitors, phoslactomycins. Effects on the cytoskeleton in NIH/3T3 cells.

  1999cites this paper
• Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinase.

  1999cites this paper
• Biochemical characterization of a casein kinase I-like actin kinase responsible for the actin-induced suppression of casein kinase II activity in vitro.

  1999cites this paper
• The crystal structure of the Physarum polycephalum actin–fragmin kinase: an atypical protein kinase with a specialized substrate‐binding domain

  1999cites this paper
• Dry stress-induced phosphorylation of Physarum actin.

  1998cites this paper
• Rho-associated kinase phosphorylates desmin, the myogenic intermediate filament protein, at unique amino-terminal sites.

  1998cites this paper
• Molecular cloning, over-expression, developmental regulation and immunolocalization of fragminP, a gelsolin-related actin-binding protein from Physarum polycephalum plasmodia.

  1997cites this paper
• In vivo phosphorylation of actin in Physarum polycephalum. Study of the substrate specificity of the actin-fragmin kinase.

  1996cites this paper
• Fragmin, a microfilament regulatory protein from Physarum polycephalum, is phosphorylated by casein kinase II-type enzymes.

  1996cites this paper
• A novel type of protein kinase phosphorylates actin in the actin‐fragmin complex.

  1996cites this paper
• Microfilament organization and actin-binding proteins

  1995cites this paper
• ADP-ribosylation of Arg28 and Arg206 on the actin molecule by chicken arginine-specific ADP-ribosyltransferase.

  1995cites this paper
• Actin-binding proteins in cell motility.

  1994cites this paper
• Comparative effects of hepatocyte growth factor and epidermal growth factor on motility, morphology, mitogenesis, and signal transduction of primary rat hepatocytes

  1994cites this paper
• Identification of sites of serine and threonine phosphorylation via site-directed mutagenesis--site transformation versus site elimination.

  1994cites this paper
• Analysis of Microfilament Organization and Contractile Activities in Physarum

  1994cites this paper
• Purification and partial amino acid sequence of the actin-fragmin kinase from Physarum polycephalum.

  1993cites this paper
• Life at the leading edge: the formation of cell protrusions.

  1993cites this paper
• Understanding the cortex of crawling cells: insights from Dictyostelium.

  1993cites this paper
• gCap39 is phosphorylated. Stimulation by okadaic acid and preferential association with nuclei.

  1993cites this paper
• Cross-talk between adenylate cyclase activation and tyrosine phosphorylation leads to modulation of the actin cytoskeleton and to acute progesterone secretion in ovarian granulosa cells.

  1993cites this paper
• F-actin capping proteins.

  1993cites this paper
• EDGE: The Formation of Cell Protrusions

  1993cites this paper
• Differences in vimentin distribution in glial cells in culture revealed with an antibody against a phosphorylated epitope

  1993cites this paper
• On the crawling of animal cells.

  1993cites this paper
• Identification of actin kinase activity in purified fragmin—actin complex

  1992cites this paper