A Functional Comparison of the 3xP3 Promoter by Recombinase-Mediated Cassette Exchange in Drosophila and a Tephritid Fly, Anastrepha suspensa

Transposable elements are widely used as vectors for integrating transgenes into the genome of insects. However, the random nature of transposon vector integrations often results in mutations and makes transgene expression subject to variable genomic position effects. This makes reliable quantitative comparisons of different transgenes difficult and development of highly fit transgenic strains laborious. Tools for site-specific transgene targeting are essential for functional genomic comparisons and to develop the most advanced transgenic insect strains for applied use. Here we describe a recombinase-mediated cassette exchange gene targeting system based on Cre/loxP that is highly efficient in Drosophila, and for the first time in a non-drosophilid, the tephritid fly, Anastrepha suspensa. This system allowed a comparison of the Drosophila constitutive polyubiquitin promoter and the artificial 3xP3 tissue-specific promoter in the same genomic context within each species, showing that the widely used 3xP3 promoter is apparently nonfunctional in the tephritid fly.

• Publication year

  2013

• Venue

  G3: Genes, Genomes, Genetics

• Publication date

  2013-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1534/g3.112.005488PMID 23550127PMCID 3618355
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A transgenic embryonic sexing system for Anastrepha suspensa (Diptera: Tephritidae).

  2012cited by this paper
• Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange

  2012cited by this paper
• Strategy for enhanced transgenic strain development for embryonic conditional lethality in Anastrepha suspensa

  2012cited by this paper
• piggybac- and PhiC31-Mediated Genetic Transformation of the Asian Tiger Mosquito, Aedes albopictus (Skuse)

  2010cited by this paper
• Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae)

  2009cited by this paper
• Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

  2009cited by this paper
• Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

  2009cited by this paper
• Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata

  2009cited by this paper
• A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila

  2008cited by this paper
• Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

  2007cited by this paper
• High efficiency site‐specific genetic engineering of the mosquito genome

  2006influential reference
• P[acman]: A BAC Transgenic Platform for Targeted Insertion of Large DNA Fragments in D. melanogaster

  2006cited by this paper
• Site-specific transgenesis by Cre-mediated recombination in Drosophila

  2005cited by this paper
• Site-specific genomic targeting in Drosophila.

  2005cited by this paper
• Insect transgenesis by site-specific recombination

  2005cited by this paper
• Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster

  2004cited by this paper
• Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.

  2004cited by this paper
• Germline transformation of the butterfly Bicyclus anynana

  2004cited by this paper
• piggyBac‐mediated germline transformation in the beetle Tribolium castaneum

  2003cited by this paper
• Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker

  2003cited by this paper
• 3xP3-EGFP marker facilitates screening for transgenic silkworm Bombyx mori L. from the embryonic stage onwards.

  2002cited by this paper
• Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites.

  2002cited by this paper
• Characteristics of the Cells-Producents of Thymic Chemotactic Factor for Bone Marrow Stem Elements

  2002cited by this paper
• The genetic control of eye development and its implications for the evolution of the various eye-types.

  2002cited by this paper
• Prospects for Using Genetic Transformation for Improved SIT and New Biocontrol Methods

  2002cited by this paper
• Genetic transformation of the housefly Musca domestica with the lepidopteran derived transposon piggyBac

  2001cited by this paper
• Efficient transformation of the yellow fever mosquito Aedes aegypti using the piggyBac transposable element vector pBac[3xP3-EGFP afm].

  2001cited by this paper
• Highly sensitive, fluorescent transformation marker for Drosophila transgenesis

  2000cited by this paper
• Insect transgenesis: methods and applications.

  2000cited by this paper
• A versatile vector set for animal transgenesis

  2000cited by this paper
• An Introduction to the History and Methodology of Insect Gene Transfer

  2000cited by this paper
• Genetic techniques: A universal marker for transgenic insects

  1999cited by this paper
• Germline transformation of Drosophila melanogaster with the piggyBac transposon vector

  1999cited by this paper
• Direct regulation of rhodopsin 1 by Pax-6/eyeless in Drosophila: evidence for a conserved function in photoreceptors.

  1997cited by this paper
• Transgene Coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila.

  1996cited by this paper
• Drosophila: A practical approach : Edited by D.B. Roberts IRL Press; Oxford, Washington DC, 1986 xix + 295 pages. Paperback £16.50/$30, hardback £26/$47

  1987cited by this paper
• Site-specific recombination of the yeast plasmid two-micron circle: intermediates in the binding process.

  1986cited by this paper
• Rosy-like Mutant of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae), and Its Potential for Use in a Genetic Sexing Program

  1982cited by this paper

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  2025cites this paper
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  2024cites this paper
• Developing genetic tools to control the Oriental fruit fly: Potential approaches and target genes

  2024cites this paper
• A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species

  2024cites this paper
• Transgenic black soldier flies for production of carotenoids.

  2024cites this paper
• Validation of a heat-inducible Ixodes scapularis HSP70 promoter and developing a tick-specific 3xP3 promoter sequence in ISE6 cells

  2023cites this paper
• Improved piggyBac Transformation with Capped Transposase mRNA in Pest Insects

  2023cites this paper
• Techniques for Identifying and Sorting Transgenic Mosquito Larvae.

  2023cites this paper
• Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives

  2022cites this paper
• Electroporation of His-Cre fusion protein triggers a specific recombinase-mediated cassette exchange in HEK 293T cells.

  2022cites this paper
• Conditional gene expression in invertebrate animal models.

  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2019cites this paper
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  2019cites this paper
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  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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  2016cites this paper
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  2016cites this paper
• Enhancing the stability and ecological safety of mass‐reared transgenic strains for field release by redundant conditional lethality systems

  2016cites this paper
• Genome Editing: From Drosophila to Non-Model Insects and Beyond.

  2016cites this paper
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  2016cites this paper
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  2015cites this paper
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  2015cites this paper
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  2015cites this paper
• Not all GMOs are crop plants: non-plant GMO applications in agriculture

  2014influential citation
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  2014cites this paper
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