Korean Red Ginseng Suppresses Metastasis of Human Hepatoma SK-Hep1 Cells by Inhibiting Matrix Metalloproteinase-2/-9 and Urokinase Plasminogen Activator

Korean red ginseng and ginsenosides have been claimed to possess wide spectrum of medicinal effects, of which anticancer effect is one. The present study was undertaken to investigate the antimetastatic effect of Korean red ginseng on human hepatoma as well as possible mechanisms. The inhibitory effect of the water extract of Korean red ginseng (WKRG) on the invasion and motility of SK-Hep1 cells was evaluated by the Boyden chamber assay in vitro. Without causing cytotoxicity, WKRG exerted a dose-dependent inhibitory effect on the invasion and motility, but not adhesion, of highly metastatic SK-Hep1 cells. Zymography analyses revealed significant downregulating effects on MMP-2, MMP-9, and uPA activities in SK-Hep1 cells. Western blot analyses also showed that WKRG treatment caused dose-dependent decreases in MMP-2 and MMP-9 protein expressions. Moreover, WKRG increased the levels of TIMP-1, TIMP-2, and PAI-1. The present study not only demonstrated that invasion and motility of cancer cells were inhibited by WKRG, but also indicated that such effects were likely associated with the decrease in MMP-2/-9 and uPA expressions of SK-Hep1 cells.

• Publication year

  2012

• Venue

  Evidence-Based Complementary and Alternative Medicine

• Publication date

  2012-04-17

• Fields of study

  Medicine

• Identifiers
  DOI 10.1155/2012/965846PMID 22570673PMCID 3337628
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• In vitro anti-inflammatory and anti-cancer activities of Cuscuta reflexa Roxb.

  2011cited by this paper
• Epigenetic regulation of matrix metalloproteinases and their collagen substrates in cancer

  2011cited by this paper
• Ginsenoside composition and antiproliferative activities of explosively puffed ginseng (Panax ginseng C.A. Meyer).

  2010cited by this paper
• Asian ginseng extract inhibits in vitro and in vivo growth of mouse lewis lung carcinoma via modulation of ERK‐p53 and NF‐κB signaling

  2010cited by this paper
• Hispolon suppresses SK-Hep1 human hepatoma cell metastasis by inhibiting matrix metalloproteinase-2/9 and urokinase-plasminogen activator through the PI3K/Akt and ERK signaling pathways.

  2010cited by this paper
• Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions.

  2010cited by this paper
• Benzyl isothiocyanate (BITC) inhibits migration and invasion of human colon cancer HT29 cells by inhibiting matrix metalloproteinase-2/-9 and urokinase plasminogen (uPA) through PKC and MAPK signaling pathway.

  2010cited by this paper
• Metabolism of Ginsenosides to Bioactive Compounds by Intestinal Microflora and Its Industrial Application

  2009cited by this paper
• Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts.

  2009cited by this paper
• Molecular mechanisms of ginsenoside Rp1-mediated growth arrest and apoptosis.

  2009cited by this paper
• Journal of Ethnopharmacology简介

  2008cited by this paper
• L-Carnosine Inhibits Metastasis of SK-Hep-1 Cells by Inhibition of Matrix Metaoproteinase-9 Expression and Induction of an Antimetastatic Gene, nm23-H1

  2008cited by this paper
• Repression of matrix metalloproteinase gene expression by ginsenoside Rh2 in human astroglioma cells.

  2007cited by this paper
• Lycopene inhibits matrix metalloproteinase-9 expression and down-regulates the binding activity of nuclear factor-kappa B and stimulatory protein-1.

  2007cited by this paper
• Antrodia camphorata extract induces replicative senescence in superficial TCC, and inhibits the absolute migration capability in invasive bladder carcinoma cells.

  2007cited by this paper
• Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer.

  2007cited by this paper
• Phenylethyl isothiocyanate and its N-acetylcysteine conjugate suppress the metastasis of SK-Hep1 human hepatoma cells.

  2006cited by this paper
• The angiosuppressive effects of 20(R)- ginsenoside Rg3.

  2006cited by this paper
• Caspase-3-dependent protein kinase C delta activity is required for the progression of Ginsenoside-Rh2-induced apoptosis in SK-HEP-1 cells.

  2005cited by this paper
• Effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells

  2004cited by this paper
• Association of suppression of extracellular signal-regulated kinase phosphorylation by epigallocatechin gallate with the reduction of matrix metalloproteinase activities in human fibrosarcoma HT1080 cells.

  2003cited by this paper
• Integrin αvβ3/Vitronectin Interaction Affects Expression of the Urokinase System in Human Ovarian Cancer Cells*

  2001cited by this paper
• Recent advances in inhibitors of matrix metalloproteinases for cancer therapy.

  1999cited by this paper
• Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2.

  1994influential reference

• Natural Products as Regulators against Matrix Metalloproteinases for the Treatment of Cancer

  2024cites this paper
• Intramuscular fat formation in fetuses and the effect of increased protein intake during pregnancy in Hanwoo cattle

  2023cites this paper
• In-vitro evaluation of immunomodulatory activity of sulphation-modified total ginsenosides derivative-3

  2023cites this paper
• Anti-MDA5 antibody-positive dermatomyositis with severe cutaneous ulcers

  2022cites this paper
• Cytotoxicity of Mahanimbine from Curry Leaves in Human Breast Cancer Cells (MCF-7) via Mitochondrial Apoptosis and Anti-Angiogenesis

  2022cites this paper
• Ginseng Seed Oil Inhibits the Growth of Estrogen Receptor-positive Breast Cancer Cells

  2020cites this paper
• The G115 standardized ginseng extract: an example for safety, efficacy, and quality of an herbal medicine

  2019cites this paper
• Methyl Linderone Suppresses TPA-Stimulated IL-8 and MMP-9 Expression Via the ERK/STAT3 Pathway in MCF-7 Breast Cancer Cells

  2019cites this paper
• 7‐Methoxy‐luteolin‐8‐C‐β‐6‐deoxy‐xylo‐pyranos‐3‐uloside exactly (mLU8C‐PU) isolated from Arthraxon hispidus inhibits migratory and invasive responses mediated via downregulation of MMP‐9 and IL‐8 expression in MCF‐7 breast cancer cells

  2018cites this paper
• Actinidia callosa var. callosa suppresses metastatic potential of human hepatoma cell SK-Hep1 by inhibiting matrix metalloproteinase-2 through PI3K/Akt and MAPK signaling pathways

  2018cites this paper
• Orientin inhibits invasion by suppressing MMP-9 and IL-8 expression via the PKCα/ ERK/AP-1/STAT3-mediated signaling pathways in TPA-treated MCF-7 breast cancer cells.

  2018cites this paper
• Effect of Korean Red Ginseng extract on colorectal lung metastasis through inhibiting the epithelial–mesenchymal transition via transforming growth factor-β1/Smad-signaling-mediated Snail/E-cadherin expression

  2017cites this paper
• Anticancer Effects of Colchicine on Hypopharyngeal Cancer.

  2017cites this paper
• Anticancer effect of 20(S)-ginsenoside Rh2 on HepG2 liver carcinoma cells: Activating GSK-3β and degrading β-catenin.

  2016cites this paper
• Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9.

  2015cites this paper
• Effect of Korean Red Ginseng on Artificial Sand Dust (ASD) Induced Allergic Lung Inflammation

  2014cites this paper
• Ginseng treatment reverses obesity and related disorders by inhibiting angiogenesis in female db/db mice.

  2014cites this paper
• The Role of Angiogenesis in Obesity

  2014cites this paper
• Korean Red Ginseng Tonic Extends Lifespan in D. melanogaster

  2013cites this paper
• Luteolin 8-C-β-fucopyranoside inhibits invasion and suppresses TPA-induced MMP-9 and IL-8 via ERK/AP-1 and ERK/NF-κB signaling in MCF-7 breast cancer cells.

  2013cites this paper
• Korean red ginseng (Panax ginseng) prevents obesity by inhibiting angiogenesis in high fat diet-induced obese C57BL/6J mice.

  2013cites this paper
• Ginseng and Its Active Components Ginsenosides Inhibit Adipogenesis in 3T3-L1 Cells by Regulating MMP-2 and MMP-9

  2012cites this paper
• Up-Regulation of TIMP-1 by Genipin Inhibits MMP-2 Activities and Suppresses the Metastatic Potential of Human Hepatocellular Carcinoma

  2012cites this paper
• Title Up-regulation of TIMP-1 by genipin inhibits MMP-2 activities and suppresses the metastatic potential of human hepatocellular carcinoma

  2012cites this paper