Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues

Abstract. Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450- to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μa and reduced scattering coefficient μ′s were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

• Publication year

  2013

• Venue

  Journal of Biomedical Optics

• Publication date

  2013-11-01

• Fields of study

  Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.1117/1.JBO.18.11.117010PMID 24247811
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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