Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore

BackgroundIn Singapore, an outbreak of fungal keratitis caused by members of the Fusarium solani species complex (FSSC) was identified in March 2005 to May 2006 involving 66 patients. Epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens solutions were risk factors.MethodsWe assessed the genetic diversity of the isolates using AFLP, Rep-PCR, and ERIC-PCR and compared the usefulness of these typing schemes to characterize the isolates.ResultsAFLP was the most discriminative typing scheme and appears to group FSSC from eye infections and from other infections differently.ConclusionThere was a high genomic heterogeneity among the isolates confirming that this was not a point source outbreak.

• Publication year

  2008

• Venue

  BMC Infectious Diseases

• Publication date

  2008-07-15

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/1471-2334-8-92PMID 18627616PMCID 2483985
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Phylogenetic Diversity and Microsphere Array-Based Genotyping of Human Pathogenic Fusaria, Including Isolates from the Multistate Contact Lens-Associated U.S. Keratitis Outbreaks of 2005 and 2006

  2007influential reference
• Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State

  2007cited by this paper
• Risk factors for contact lens-related fusarium keratitis: a case-control study in Singapore.

  2007cited by this paper
• Multistate outbreak of Fusarium keratitis associated with use of a contact lens solution.

  2006influential reference
• Growth and Survival of Fusarium solani-F. oxysporum Complex on Stressed Multipurpose Contact Lens Care Solution Films on Plastic Surfaces In Situ and In Vitro

  2006cited by this paper
• Members of the Fusarium solani Species Complex That Cause Infections in Both Humans and Plants Are Common in the Environment

  2006influential reference
• An outbreak of Fusarium keratitis associated with contact lens wear in Singapore.

  2006cited by this paper
• Use of the DiversiLab System for Species and Strain Differentiation of Fusarium Species Isolates

  2005cited by this paper
• Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR

  2005cited by this paper
• Endophthalmitis Caused by Fusarium proliferatum

  2005cited by this paper
• Genotyping of 44 Isolates of Fusarium solani, the Main Agent of Fungal Keratitis in Brazil

  2004cited by this paper
• Genetic Diversity of Human Pathogenic Members of the Fusarium oxysporum Complex Inferred from Multilocus DNA Sequence Data and Amplified Fragment Length Polymorphism Analyses: Evidence for the Recent Dispersion of a Geographically Widespread Clonal Lineage and Nosocomial Origin

  2004cited by this paper
• Molecular phylogeny of Fusarium species by AFLP fingerprint

  2003cited by this paper
• Fusariosis associated with pathogenic fusarium species colonization of a hospital water system: a new paradigm for the epidemiology of opportunistic mold infections.

  2001cited by this paper
• Determining Confidence Intervals When Measuring Genetic Diversity and the Discriminatory Abilities of Typing Methods for Microorganisms

  2001cited by this paper
• Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals.

  2001cited by this paper
• The Measurement of Diversity

  2001cited by this paper
• Identification of Fusarium Species Involved in Human Infections by 28S rRNA Gene Sequencing

  1999cited by this paper
• How to select and interpret molecular strain typing methods for epidemiological studies of bacterial infections: a review for healthcare epidemiologists. Molecular Typing Working Group of the Society for Healthcare Epidemiology of America.

  1997cited by this paper
• How to Select and Interpret Molecular Strain Typing Methods for Epidemiological Studies of Bacterial Infections A Review for Healthcare Epidemiologists

  1997cited by this paper
• Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems.

  1996cited by this paper
• AFLP: a new technique for DNA fingerprinting.

  1995cited by this paper
• Taxonomy, biology, and clinical aspects of Fusarium species

  1994cited by this paper
• Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.

  1991cited by this paper
• Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity

  1988cited by this paper

• Novel amplified fragment length polymorphism (AFLP) markers for typing medically relevant Fusarium and allied fusarioid genera.

  2025cites this paper
• Disinfectants in a Hemodialysis Setting: Antifungal Activity Against Aspergillus and Fusarium Planktonic and Biofilm Cells and the Effect of Commercial Peracetic Acid Residual in Mice

  2021cites this paper
• A 23 bp cyp51A Promoter Deletion Associated With Voriconazole Resistance in Clinical and Environmental Isolates of Neocosmospora keratoplastica

  2020cites this paper
• Microbial Keratitis in Taiwan: A 20-Year update.

  2019cites this paper
• Fusarium: The versatile pathogen

  2018cites this paper
• Use of AFLP for the study of eukaryotic pathogens affecting humans.

  2017cites this paper
• Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?

  2017influential citation
• Belowground Defence Strategies in Plants

  2016cites this paper
• Root Interactions with Nonpathogenic Fusarium oxysporum

  2016cites this paper
• Typing of Fungi in an Outbreak Setting: Lessons Learned

  2015cites this paper
• ADVANCES IN DIAGNOSIS OF INVASIVE FUNGAL INFECTIONS

  2015cites this paper
• Adaptation of Fusarium oxysporum and Fusarium dimerum to the specific aquatic environment provided by the water systems of hospitals.

  2015cites this paper
• Avaliação de método molecular para detecção direta de Fusarium

  2015cites this paper
• Fusarium napiforme systemic infection: case report with molecular characterization and antifungal susceptibility tests

  2014cites this paper
• Comparação de técnicas moleculares para identificação das espécies de fusarium de amostras clínicas

  2014cites this paper
• Typage moléculaire du complexe d'espèces Fusarium solani et détermination de son mécanisme de résistance au voriconazole : Molecular typing of Fusarium solani species complex and determination of its resistance mechanism to voriconazole

  2013cites this paper
• Efficacy of Care Solutions Against Contact Lens-Associated Fusarium Biofilms

  2012cites this paper
• Genetic diversity of Fusarium oxysporum f. sp. cubense isolates from Malaysia.

  2010cites this paper
• Fungal Systematics and Evolution

  year unknowncites this paper