microRNA‐96 acts as a tumor suppressor gene in human osteosarcoma via target regulation of EZRIN

Aims: microRNA‐96 (miR‐96) is considered as a tumor suppressor in multiple malignancies. Some studies have indicated that EZRIN could be regulated by the miR‐96 in several kinds of cancer cells. However, the function of miR‐96 in human osteosarcoma has not been investigated intensively so far. In this study, we mainly explored the relationship between miR‐96 and the expression of EZRIN in human osteosarcoma cells. Main methods: The levels of miR‐96 and EZRIN in osteosarcoma tissues and paracancerous tissues of patients were evaluated with qPCR. The targeted regulation between miR‐96 and EZRIN was further confirmed with Dual luciferase assay. Overexpression of miR‐96 and EZRIN was induced in human osteosarcoma cell line, and the changing of cell viability was analyzed to determine the role of miR‐96 in human osteosarcoma in vitro and in vivo. Key findings: Our results showed that the expression level of miR‐96 was much lower in human osteosarcoma tissues compared with human paracancerous tissues. Dual luciferase assay indicated that EZRIN as the direct target of miR‐96 in osteosarcoma cells. Besides, the up‐regulation of miR‐96 could inhibit cell proliferation, cell migration and invasion, tumor formation ability, and increase the percentage of cell apoptosis in osteosarcoma cells through inhibiting EZRIN. Significance: Therefore, our study indicated that miR‐96 hold an important role for the development and progress of human osteosarcoma, and this miRNA might become a novel target in the diagnosis and treatment of human osteosarcoma and even other cancers.

• Publication year

  2018

• Venue

  Life Science

• Publication date

  2018-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.lfs.2018.04.012PMID 29656060
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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