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Suppression of miR-26a attenuates physiological disturbances arising from exposure of Nile tilapia (Oreochromis niloticus) to ammonia

Yan Zhao,Haotian Zhou,C. Ayisi,Yan Wang,Jun Wang,Xiaowu Chen,Jinling Zhao

Published 2018 in Biology Open

ABSTRACT

ABSTRACT MicroRNAs may affect stress responses because they act as rapid responders at the post-translation level. In this study, we found that miR-26a is abundantly expressed in the brain and gill tissues of tilapia. Expression of miR-26a in the brain decreased significantly with increasing ammonia concentrations using stem-loop qPCR. To analyze the function of miRNA in vivo, miR-26a was stably knocked down with an antagomir in tilapia. Following ammonia challenge, miR-26a antagomir treatment significantly suppressed blood ammonia/[Cl−]/[K+] concentration and the reactive oxygen species production, while it markedly enhanced glutamine accumulation and antioxidant enzyme activity in the brain of tilapia, indicating that miR-26a may be involved in the remission of physiological disturbances resulting from ammonia stress. We strongly conclude that there is a direct link between miR-26a and the responses to ammonia in tilapia. Furthermore, bioinformatics analysis and luciferase assays demonstrated that miR-26a regulates HSP70 (heat shock protein 70) and GS (glutamine synthetase) expression by targeting their 3′-UTR and that the suppression of miR-26a could increase the intracellular level of HSP70 and GS in vivo. Summary: Our work increases the available information about the regulation of miR-26a and indicates that miR-26a may be involved in the remission of physiological disorders upon ammonia stress in tilapia.

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