Bacterial community structure is indicative of chemical inputs in the Upper Mississippi River

Local and regional associations between bacterial communities and nutrient and chemical concentrations were assessed in the Upper Mississippi River in Minnesota to determine if community structure was associated with discrete types of chemical inputs associated with different land cover. Bacterial communities were characterized by Illumina sequencing of the V6 region of 16S rDNA and compared to >40 chemical and nutrient concentrations. Local bacterial community structure was shaped primarily by associations among bacterial orders. However, order abundances were correlated regionally with nutrient and chemical concentrations, and were also related to major land coverage types. Total organic carbon and total dissolved solids were among the primary abiotic factors associated with local community composition and co-varied with land cover. Escherichia coli concentration was poorly related to community composition or nutrient concentrations. Abundances of 14 bacterial orders were related to land coverage type, and seven showed significant differences in abundance (P ≤ 0.046) between forested or anthropogenically-impacted sites. This study identifies specific bacterial orders that were associated with chemicals and nutrients derived from specific land cover types and may be useful in assessing water quality. Results of this study reveal the need to investigate community dynamics at both the local and regional scales and to identify shifts in taxonomic community structure that may be useful in determining sources of pollution in the Upper Mississippi River.

• Publication year

  2014

• Venue

  Frontiers in Microbiology

• Publication date

  2014-10-08

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fmicb.2014.00524PMID 25339945PMCID 4189419
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Abundances of 14 bacterial orders were related to land cover type, and seven differed significantly between forested and anthropogenically impacted sites.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Escherichia coli concentration was poorly related to community composition or nutrient concentrations.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Total organic carbon and total dissolved solids were among the primary abiotic factors associated with local community composition and co-varied with land cover.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Order abundances were correlated regionally with nutrient and chemical concentrations and were related to land cover.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Local bacterial community structure was shaped primarily by associations among bacterial orders.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

• bacterial community structure

  community structure

  The composition and organization of bacterial taxa in the sampled river communities.

  Aliases: community composition

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• bacterial orders

  taxonomic rank

  Taxonomic groups at the order rank used to summarize bacterial diversity in the samples.

  Aliases: order abundances

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• escherichia coli concentration

  measurement

  The measured abundance of Escherichia coli in the river water samples.

  Aliases: E. coli concentration

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• land cover

  environmental factor

  The dominant surrounding land-cover classes associated with sampling sites along the river.

  Aliases: land coverage types, land cover type

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• nutrient and chemical concentrations

  measurement

  Measured concentrations of nutrients and chemical variables in the river water samples.

  Aliases: chemical concentrations, nutrient concentrations

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• total dissolved solids

  measurement

  A measure of dissolved material present in the sampled water.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• total organic carbon

  measurement

  A water-quality measure of organic carbon content in the sampled water.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

• Human and Environmental Impacts on River Sediment Microbial Communities

  2014cited by this paper
• A Microbial Signature Approach to Identify Fecal Pollution in the Waters Off an Urbanized Coast of Lake Michigan

  2013cited by this paper
• Wastewater Treatment Effluent Reduces the Abundance and Diversity of Benthic Bacterial Communities in Urban and Suburban Rivers

  2013influential reference
• Taxonomic Classification of Bacterial 16S rRNA Genes Using Short Sequencing Reads: Evaluation of Effective Study Designs

  2013cited by this paper
• Microbial biogeography of the North Sea during summer

  2013influential reference
• Comparison of Sequencing Utility Programs

  2013cited by this paper
• Application of Illumina next‐generation sequencing to characterize the bacterial community of the Upper Mississippi River

  2013influential reference
• Comparative Metagenomics of Toxic Freshwater Cyanobacteria Bloom Communities on Two Continents

  2012cited by this paper
• Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

  2012cited by this paper
• Dispersal-mediated trophic interactions can generate apparent patterns of dispersal limitation in aquatic metacommunities.

  2012cited by this paper
• Influence of Land Use and Nutrient Flux on Metabolic Activity of E. coli O157 in River Water

  2012cited by this paper
• Integrated online system for a pyrosequencing-based microbial source tracking method that targets Bacteroidetes 16S rDNA.

  2012influential reference
• Temporal variability in the diversity and composition of stream bacterioplankton communities.

  2012cited by this paper
• Predicting bacterial community assemblages using an artificial neural network approach

  2012cited by this paper
• Bacterial pathogens in Hawaiian coastal streams--associations with fecal indicators, land cover, and water quality.

  2011cited by this paper
• Bayesian community-wide culture-independent microbial source tracking

  2011influential reference
• UCHIME improves sensitivity and speed of chimera detection

  2011cited by this paper
• Spatial variability in airborne bacterial communities across land-use types and their relationship to the bacterial communities of potential source environments

  2011cited by this paper
• Spatial and temporal relationships between water quality and land use in northern Georgia, USA

  2011cited by this paper
• Spatial variability overwhelms seasonal patterns in bacterioplankton communities across a river to ocean gradient

  2011cited by this paper
• Extended local similarity analysis (eLSA) of microbial community and other time series data with replicates

  2011influential reference
• Completion of the 2006 National Land Cover Database for the conterminous United States.

  2011cited by this paper
• Watershed Urbanization Alters the Composition and Function of Stream Bacterial Communities

  2011cited by this paper
• Defining seasonal marine microbial community dynamics

  2011influential reference
• Bacterioplankton Community Variation Across River to Ocean Environmental Gradients

  2011cited by this paper
• Whole Catchment Land Cover Effects on Water Quality in the Lower Kaskaskia River Watershed

  2011cited by this paper
• Ecological consequences of bacterioplankton lifestyles: changes in concepts are needed.

  2010cited by this paper
• Relationship Between Soil Properties and Patterns of Bacterial β-diversity Across Reclaimed and Natural Boreal Forest Soils

  2010cited by this paper
• Use of barcoded pyrosequencing and shared OTUs to determine sources of fecal bacteria in watersheds.

  2010cited by this paper
• Quantifying the effect of land use land cover change on increasing discharge in the Upper Mississippi River

  2010cited by this paper
• Contaminants in the Upper Mississippi River: historic trends, responses to regulatory controls, and emerging concerns

  2010influential reference
• Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities

  2009cited by this paper
• The seasonal structure of microbial communities in the Western English Channel.

  2009cited by this paper
• Links between resources, C metabolism and the major components of bacterioplankton community structure across a range of freshwater ecosystems.

  2009cited by this paper
• A sediment record of trace metal loadings in the Upper Mississippi River

  2009cited by this paper
• The Ribosomal Database Project: improved alignments and new tools for rRNA analysis

  2008cited by this paper
• Simultaneous quantification of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and pharmaceuticals and personal care products (PPCPs) in Mississippi river water, in New Orleans, Louisiana, USA.

  2007cited by this paper
• SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB

  2007cited by this paper
• Computational Inference of Neural Information Flow Networks

  2006influential reference
• Pharmaceutical and personal care products (PPCPs) in urban receiving waters.

  2006influential reference
• Local similarity analysis reveals unique associations among marine bacterioplankton species and environmental factors

  2006influential reference
• Microbial diversity in the deep sea and the underexplored “rare biosphere”

  2006cited by this paper
• UniFrac: a New Phylogenetic Method for Comparing Microbial Communities

  2005cited by this paper
• Argonaute—a database for gene regulation by mammalian microRNAs

  2005cited by this paper
• Indigenous soil bacteria and low moisture may limit but allow faecal bacteria to multiply and become a minor population in tropical soils.

  2004cited by this paper
• Cytoscape: a software environment for integrated models of biomolecular interaction networks.

  2003influential reference
• The relationship of land use practices to surface water quality in the Upper Oconee Watershed of Georgia

  2000cited by this paper
• History of Mercury Loading in the Upper Mississippi River Reconstructed from the Sediments of Lake Pepin

  1999cited by this paper
• UNITED STATES ENVIRONMENTAL PROTECTION AGENCY - _

  1993cited by this paper
• Non‐parametric multivariate analyses of changes in community structure

  1993influential reference
• Nonpoint source contamination of the Mississippi River and its tributaries by herbicides

  1993influential reference
• Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.

  1992influential reference
• An Ordination of the Upland Forest Communities of Southern Wisconsin

  1957cited by this paper

• Microbial diversity patterns and extracellular enzyme activities of river sediment in different geomorphic regions of the Loess Plateau

  2025cites this paper
• The assembly processes and network characteristics of bacterial, fungal and archaeal communities in the middle Yangtze River and river-connected lakes

  2025cites this paper
• Building Predictive Understanding of the Activated Sludge Microbiome by Bridging Microbial Growth Kinetics and Microbial Population Dynamics.

  2025cites this paper
• Impact of organic matter and inorganic contaminants on physicochemical characteristics in riverine sediments

  2025cites this paper
• Bayesian networks for network inference in biology

  2025cites this paper
• Diversity and biogeography of bacterial community in the Ili River network varies locally and regionally.

  2024cites this paper
• Riverine bacterial communities are more shaped by species sorting in intensive urban and agricultural watersheds

  2024cites this paper
• Microbiological and physicochemical water quality assessments of the Upper Basin Litany River, Lebanon

  2024cites this paper
• Comparison of Yamuna (India) and Mississippi River (United States of America) bacterial communities reveals greater diversity below the Yamunotri Glacier

  2024cites this paper
• Assessing the impact of inland navigation on the faecal pollution status of large rivers: A novel integrated field approach.

  2024cites this paper
• Microbial communities change along the 300 km length of the Grand River for extreme high- and low-flow regimes.

  2024cites this paper
• Linking pharmaceutical residues to dissolved organic matter and aquatic bacterial communities in a highly urbanized bay.

  2023cites this paper
• Exploring the efficacy of metabarcoding and non-target screening for detecting treated wastewater.

  2023cites this paper
• Nontarget Chemical Composition of Surface Waters May Reflect Ecosystem Processes More than Discrete Source Contributions.

  2023cites this paper
• Wastewater treatment plant effluent discharge decreases bacterial community diversity and network complexity in urbanized coastal sediment.

  2023cites this paper
• Bacterioplankton community indicators for seasonal variation in a fragmented subtropical river

  2022cites this paper
• Allometric releases of nitrogen and phosphorus from sediments mediated by bacteria determines water eutrophication in coastal river basins of Bohai Bay.

  2022cites this paper
• Changes in protist communities in drainages across the Pearl River Delta under anthropogenic influence.

  2021cites this paper
• Determining the microbial and chemical contamination in Ecuador’s main rivers

  2021cites this paper
• Characterization of the bacterioplankton community and the influencing factors in the upper reaches of the Han River basin

  2021cites this paper
• Assessing the in situ bacterial diversity and composition at anthropogenically active sites using the environmental DNA (eDNA).

  2021cites this paper
• Sedimentary microeukaryotes reveal more dispersal limitation and form networks with less connectivity than planktonic microeukaryotes in a highly regulated river

  2021cites this paper
• Effect of Water Chemistry, Land Use Patterns, and Geographic Distances on the Spatial Distribution of Bacterioplankton Communities in an Anthropogenically Disturbed Riverine Ecosystem

  2021cites this paper
• Next Generation High Throughput Sequencing to Assess Microbial Communities: An Application Based on Water Quality

  2021cites this paper
• Long-term agricultural contamination shaped diversity response of sediment microbiome.

  2021cites this paper
• Linking bacterial community shifts with changes in the dissolved organic matter pool in a eutrophic lake.

  2020cites this paper
• Uncovering the genomic potential of the Amazon River microbiome to degrade rainforest organic matter

  2020influential citation
• Iterative Subtractive Binning of Freshwater Chronoseries Metagenomes Identifies over 400 Novel Species and their Ecologic Preferences.

  2020cites this paper
• Spatial Variation in Bacterioplankton Communities in the Pearl River, South China: Impacts of Land Use and Physicochemical Factors

  2020cites this paper
• Evaluation of gaseous substrates for microbial immobilization of contaminant mixtures in unsaturated subsurface sediments.

  2020cites this paper
• Seasonal and spatial distribution and assembly processes of bacterioplankton communities in a subtropical urban river.

  2020cites this paper
• Microbial communities can predict the ecological condition of headwater streams

  2020cites this paper
• Determination of the Microbial and Chemical Loads in Rivers from the Quito Capital Province of Ecuador (Pichincha)—A Preliminary Analysis of Microbial and Chemical Quality of the Main Rivers

  2020influential citation
• Sediment Bacterial Community Structure under the Influence of Different Domestic Sewage Types

  2020cites this paper
• Fungal community demonstrates stronger dispersal limitation and less network connectivity than bacterial community in sediments along a large river.

  2020cites this paper
• Microbial responses to unconventional oil and gas development may alter ecosystem function in headwater streams

  2020cites this paper
• Particle-attached riverine bacteriome shifts in a pollutant-resistant and pathogenic community during a Mediterranean extreme storm event.

  2020cites this paper
• Microbial Source Tracking in the Love Creek Watershed, Delaware (USA)

  2020cites this paper
• Spatial-Temporal Variation of Bacterial Communities in Sediments in Lake Chaohu, a Large, Shallow Eutrophic Lake in China

  2019cites this paper
• Bacterial community composition shaped by water chemistry and geographic distance in an anthropogenically disturbed river.

  2019cites this paper
• Coupled effects of land use pattern and hydrological regime on composition and diversity of riverine eukaryotic community in a coastal watershed of Southeast China.

  2019cites this paper
• Temporal and spatial changes in bacterial diversity in mixed use watersheds of the Great Lakes region

  2019cites this paper
• Exploring co-occurrence patterns between organic micropollutants and bacterial community structure in a mixed-use watershed.

  2019cites this paper
• Uncovering the gene machinery of the Amazon River microbiome to degrade rainforest organic matter

  2019cites this paper
• Spatial and temporal succession of bacterial communities in three artificial fishponds

  2019cites this paper
• Changes of Bacterial Communities in Response to Prolonged Hydrodynamic Disturbances in the Eutrophic Water-Sediment Systems

  2019cites this paper
• Comparative metagenomic analysis of microbial taxonomic and functional variations in untreated surface and reclaimed waters used in irrigation applications.

  2019cites this paper
• Iterative Subtractive Binning of Freshwater Chronoseries Metagenomes Identifies of over Four Hundred Novel Species and their Ecologic Preferences

  2019cites this paper
• Metagenomic analysis of bacterial and viral assemblages from a freshwater creek and irrigated field reveals temporal and spatial dynamics.

  2019cites this paper
• Benthic invertebrate and microbial biodiversity in sub-tropical urban rivers: Correlations with environmental variables and emerging chemicals.

  2019cites this paper
• Novel surfactant peptide for removal of biofilms.

  2018cites this paper
• Nutrient dynamics and stream order influence microbial community patterns along a 2914 kilometer transect of the Mississippi River

  2018influential citation
• Microbial Ecology of South Florida Surface Waters: Examining the Potential for Anthropogenic Influences

  2018cites this paper
• Iron limitation effects on nitrogen-fixing organisms with possible implications for cyanobacterial blooms

  2018cites this paper
• Effect of vitamin B12 on resilience to turbidity stress for Moina macrocopa

  2018cites this paper
• Sediment bacteria in an urban stream: Spatiotemporal patterns in community composition.

  2018cites this paper
• Dynamics of the bacterial community in a channel catfish nursery pond with a cage-pond integration system.

  2018cites this paper
• Response of Aquatic Bacterial Communities to Hydraulic Fracturing in Northwestern Pennsylvania: A Five-Year Study

  2018cites this paper
• Evaluation of the microbial and chemical load in rivers from the province of Pichincha in Ecuador

  2018cites this paper
• Nutrient dynamics and stream order influence microbial community patterns along a 2914 km transect of the Mississippi River

  2017influential citation
• Patterns of variation in diversity of the Mississippi river microbiome over 1,300 kilometers

  2017cites this paper
• Shift in bacterioplankton diversity and structure: Influence of anthropogenic disturbances along the Yarlung Tsangpo River on the Tibetan Plateau, China

  2017cites this paper
• Dynamic microbial populations along the Cuyahoga River

  2017cites this paper
• Unravelling and Reconstructing the Nexus of Salinity, Electricity, and Microbial Ecology for Bioelectrochemical Desalination.

  2017cites this paper
• Nutrient treatments alter microbial mat colonization in two glacial meltwater streams from the McMurdo Dry Valleys, Antarctica.

  2016cites this paper
• Associations between soil bacterial community structure and nutrient cycling functions in long-term organic farm soils following cover crop and organic fertilizer amendment.

  2016cites this paper
• Running title: Nutrients alter Antarctic stream communities

  2016cites this paper
• Microbial regime changes and indicators of eutrophication on the Mississippi River identified via a human-powered 2900 km transect

  2016cites this paper
• Association between the occurrence of the Thick-shelled River Mussel (Unio crassus) and macroinvertebrate, microbial, and diatom communities

  2016cites this paper
• The Evolving Science of Microbial Source Tracking

  2016cites this paper
• Sediments and Soils Act as Reservoirs for Taxonomic and Functional Bacterial Diversity in the Upper Mississippi River

  2016cites this paper
• Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

  2016cites this paper
• Sequencing Insights into Microbial Communities in the Water and Sediments of Fenghe River, China

  2016cites this paper
• Anthropogenic effects on bacterial diversity and function along a river-to-estuary gradient in Northwest Greece revealed by metagenomics.

  2016influential citation
• Title : Nutrient treatments alter microbial mat colonization in two glacial meltwater streams from the McMurdo Dry Valleys , Antarctica Running title : Nutrients alter Antarctic stream communities Author names :

  2016cites this paper
• Restructuring of the Aquatic Bacterial Community by Hydric Dynamics Associated with Superstorm Sandy

  2016cites this paper
• Evaluation of water sampling methodologies for amplicon-based characterization of bacterial community structure.

  2015cites this paper
• What difference does it make if viruses are strain-, rather than species-specific?

  2015cites this paper
• A novel bacterial community index to assess stream ecological health

  2015cites this paper
• A Delphi Technology Foresight Study: Mapping Social Construction of Scientific Evidence on Metagenomics Tests for Water Safety

  2015cites this paper
• Application of metagenomics to assess microbial communities in water and other environmental matrices

  2015cites this paper
• Spatiotemporal dynamics and determinants of planktonic bacterial and microeukaryotic communities in a Chinese subtropical river

  2015cites this paper
• datasets for the identification of fecal pollution in surface water

  2015cites this paper
• Multiple Discharges of Treated Municipal Wastewater Have a Small Effect on the Quantities of Numerous Antibiotic Resistance Determinants in the Upper Mississippi River.

  2015cites this paper
• Year-Long Metagenomic Study of River Microbiomes Across Land Use and Water Quality

  2015cites this paper
• Species sorting and seasonal dynamics primarily shape bacterial communities in the Upper Mississippi River.

  2015cites this paper
• Influence of Land Use, Nutrients, and Geography on Microbial Communities and Fecal Indicator Abundance at Lake Michigan Beaches

  2015cites this paper
• The microbiome of urban waters.

  2015cites this paper
• Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing

  2015cites this paper
• Toolbox Approaches Using Molecular Markers and 16S rRNA Gene Amplicon Data Sets for Identification of Fecal Pollution in Surface Water

  2015cites this paper
• Frequencies of heavy metal resistance are associated with land cover type in the Upper Mississippi River.

  2015cites this paper