Tetrahydroxystilbene glycoside antagonizes β-amyloid-induced inflammatory injury in microglia cells by regulating PU.1 expression

Inhibiting &bgr;-amyloid (A&bgr;)-induced microglial activation is proposed as an effective strategy for the treatment of Alzheimer’s disease. Tetrahydroxystilbene glycoside (TSG) is the main active ingredient of Polygonum multiflorum and has a wide range of biological properties, including antiinflammation. Here, we focused on the function and regulatory mechanism of TSG in A&bgr;-induced N9 and BV2 cells. The results showed that A&bgr; treatment induced the activation of microglia cells and the production of inflammatory molecules, including inducible nitric oxide synthase, nitric oxide, cyclooxygenase 2, and prostaglandin E2, which were significantly inhibited by TSG pretreatment. Furthermore, we found A&bgr; exposure increased the levels of microglial M1 markers, interleukin (IL)-1&bgr;, IL-6, and tumor necrosis factor &agr;, and the pretreatment of TSG suppressed the increase of M1 markers and enhanced the levels of M2 markers, including IL-10, brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, and arginase-1. PU.1 overexpression was found to eradicate the anti-inflammatory effects of TSG in A&bgr;-induced microglial cells. Taken together, these findings indicate that TSG attenuates A&bgr;-induced microglial activation and polarizes microglia towards M2 phenotype, which may be closely associated with the regulation of PU.1.

• Publication year

  2018

• Venue

  NeuroReport

• Publication date

  2018-06-06

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1097/WNR.0000000000001032PMID 29668503PMCID 5999375
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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