Codon harmonization – going beyond the speed limit for protein expression

No abstract is available for this paper.

• Publication year

  2018

• Venue

  FEBS Letters

• Publication date

  2018-05-01

• Fields of study

  Biology, Medicine, Computer Science

• Identifiers
  DOI 10.1002/1873-3468.13046PMID 29624661
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodium berghei/vivax

  2017cited by this paper
• Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli

  2017cited by this paper
• Widespread position-specific conservation of synonymous rare codons within coding sequences

  2017cited by this paper
• Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

  2017cited by this paper
• Multi-omic profiling of EPO-producing Chinese hamster ovary cell panel reveals metabolic adaptation to heterologous protein production.

  2016cited by this paper
• Codon Optimisation Is Key for Pernisine Expression in Escherichia coli

  2015cited by this paper
• Rationally designed, heterologous S. cerevisiae transcripts expose novel expression determinants

  2015cited by this paper
• ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization

  2015cited by this paper
• The multifaceted benefits of protein co-expression in Escherichia coli.

  2015cited by this paper
• Multi‐omic profiling of EPO‐producing Chinese hamster ovary cell panel reveals metabolic adaptation to heterologous protein production

  2015cited by this paper
• Codon Optimization Significantly Improves the Expression Level of α-Amylase Gene from Bacillus licheniformis in Pichia pastoris

  2015cited by this paper
• Roles for Synonymous Codon Usage in Protein Biogenesis.

  2015cited by this paper
• Influenza A Virus Attenuation by Codon Deoptimization of the NS Gene for Vaccine Development

  2014cited by this paper
• Speed Controls in Translating Secretory Proteins in Eukaryotes - an Evolutionary Perspective

  2014cited by this paper
• Codon Optimization OnLine (COOL): a web-based multi-objective optimization platform for synthetic gene design

  2014cited by this paper
• A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae

  2014cited by this paper
• Multiple roles of the coding sequence 5′ end in gene expression regulation

  2014cited by this paper
• Computer-aided codon-pairs deoptimization of the major envelope GP5 gene attenuates porcine reproductive and respiratory syndrome virus.

  2014cited by this paper
• Causes and Effects of N-Terminal Codon Bias in Bacterial Genes

  2013cited by this paper
• Non-optimal codon usage affects expression, structure and function of FRQ clock protein

  2013cited by this paper
• Identification and characterization of antigens as vaccine candidates against Klebsiella pneumoniae

  2013cited by this paper
• Non-optimal codon usage is a mechanism to achieve circadian clock conditionality

  2013cited by this paper
• Non-optimal codon usage affects expression , structure and function of clock protein FRQ

  2013cited by this paper
• tRNA concentration fine tunes protein solubility

  2012cited by this paper
• Shedding of TRAP by a Rhomboid Protease from the Malaria Sporozoite Surface Is Essential for Gliding Motility and Sporozoite Infectivity

  2012cited by this paper
• Engineering genes for predictable protein expression.

  2012cited by this paper
• Synonymous but not the same: the causes and consequences of codon bias

  2011cited by this paper
• Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli

  2011cited by this paper
• Codon usage: Nature's roadmap to expression and folding of proteins

  2011cited by this paper
• Secretory production of recombinant proteins in Escherichia coli.

  2010cited by this paper
• RCDI/eRCDI: a web-server to estimate codon usage deoptimization

  2010cited by this paper
• An evolutionarily conserved mechanism for controlling the efficiency of protein translation.

  2010cited by this paper
• Live Attenuated Influenza Vaccines by Computer-Aided Rational Design

  2010cited by this paper
• Differential Arginylation of Actin Isoforms Is Regulated by Coding Sequence–Dependent Degradation

  2010cited by this paper
• How the sequence of a gene can tune its translation.

  2010cited by this paper
• Coding-Sequence Determinants of Gene Expression in Escherichia coli

  2009cited by this paper
• A pause for thought along the co-translational folding pathway.

  2009cited by this paper
• Design Parameters to Control Synthetic Gene Expression in Escherichia coli

  2009cited by this paper
• Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

  2008cited by this paper
• Distance Learning for Similarity Estimation

  2008cited by this paper
• Differential translation efficiency of orthologous genes is involved in phenotypic divergence of yeast species

  2007cited by this paper
• Hearing silence: non-neutral evolution at synonymous sites in mammals

  2006cited by this paper
• Expression, Purification and Characterization of a Recombinant Plasmodium Vivax Thrombospondin Related Adhesive Protein (PvTRAP)

  2006cited by this paper
• Codon bias and heterologous protein expression.

  2004cited by this paper
• In vivo introduction of unpreferred synonymous codons into the Drosophila Adh gene results in reduced levels of ADH protein.

  2003cited by this paper
• Selective Charging of tRNA Isoacceptors Explains Patterns of Codon Usage

  2003cited by this paper
• Silent mutations affect in vivo protein folding in Escherichia coli.

  2002cited by this paper
• Papillomavirus Capsid Protein Expression Level Depends on the Match between Codon Usage and tRNA Availability

  1999cited by this paper
• Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation

  1999cited by this paper
• Functional characterization of the somatic hypermutation process leading to antibody D1.3, a high affinity antibody directed against lysozyme.

  1999cited by this paper
• Dictionary of Algorithms and Data Structures | NIST

  1998cited by this paper
• Coexpression of nuclear receptor partners increases their solubility and biological activities.

  1997cited by this paper
• Three

  1994cited by this paper
• Relationship between protein synthesis and concentrations of charged and uncharged tRNATrp in Escherichia coli.

  1990cited by this paper
• Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression

  1987cited by this paper
• The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.

  1987cited by this paper
• Codon usage can affect efficiency of translation of genes in Escherichia coli.

  1984cited by this paper

• Pichia-CLM: A language model-based codon optimization pipeline for Komagataella phaffii.

  2026cites this paper
• Systematic Evaluation of Vector Sequence Elements in Isogenic CHO Cells for Improved Antibody Production

  2025cites this paper
• Effects of single synonymous substitutions on folding efficiency demonstrate the influence of rare codons and protein structure

  2025influential citation
• Codon harmonization for improve heterologous expression of human aromatase (CYP19A1) in E. coli cells.

  2025cites this paper
• The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins—A Case Study About Producing a Rice Enzyme

  2025cites this paper
• Codon usage modulates the relationship between the burden and yield of protein overexpression

  2025cites this paper
• Codon harmonization as a way to increase the expression level of functionally active human CYP17A1

  2025cites this paper
• Strangers in a foreign land: ‘Yeastizing’ plant enzymes

  2024cites this paper
• Modeling and measuring how codon usage modulates the relationship between burden and yield during protein overexpression in bacteria

  2024cites this paper
• Development of recombinant proteins for vaccine candidates against serotypes O and A of Foot-and-Mouth Disease virus in Bangladesh

  2024cites this paper
• Profiling expression strategies for a type III polyketide synthase in a lysate-based, cell-free system

  2024cites this paper
• RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling

  2024cites this paper
• Base composition, adaptation, and evolution of goose astroviruses: codon-based investigation

  2023cites this paper
• The Effects of Codon Usage on Protein Structure and Folding.

  2023cites this paper
• Bacterial natural product discovery by heterologous expression

  2023cites this paper
• Profiling Expression Strategies for a Type III Polyketide Synthase in a Lysate-Based, Cell-free System

  2023cites this paper
• An exploratory in silico comparison of open-source codon harmonization tools

  2023cites this paper
• Expression of blue pigment synthetase a from Streptomyces lavenduale reveals insights on the effects of refactoring biosynthetic megasynthases for heterologous expression in Escherichia coli.

  2023cites this paper
• Current structure predictors are not learning the physics of protein folding

  2022cites this paper
• Porcine IGF-1R synonymous mutations in the extracellular domain affect proliferation and differentiation of skeletal muscle cells.

  2022cites this paper
• Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies.

  2022cites this paper
• Design and Cloning of Gene Encoding SLPI C-Terminal Domain in Escherichia coli TOP10

  2022cites this paper
• From the notebook to recombinant protein production in Escherichia coli: Design of expression vectors and gene cloning.

  2021cites this paper
• Exploring Codon Adjustment Strategies towards Escherichia coli-Based Production of Viral Proteins Encoded by HTH1, a Novel Prophage of the Marine Bacterium Hypnocyclicus thermotrophus

  2021influential citation
• Immuno-Efficacy of Multi-Epitope Chimeric Peptides Against Foot and Mouth Disease Virus: Potential Vaccine Candidates for Newly Emerged Serotype O and A

  2021cites this paper
• Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms

  2021cites this paper
• Current protein structure predictors do not produce meaningful folding pathways

  2021cites this paper
• Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt.

  2021cites this paper
• Heterologous expression of lytic polysaccharide monooxygenases (LPMOs). A mini-review.

  2020cites this paper
• Highly active rubiscos discovered by systematic interrogation of natural sequence diversity

  2020cites this paper
• Potential role of the X circular code in the regulation of gene expression

  2020cites this paper
• Codon Harmonization of a Kir3.1-KirBac1.3 Chimera for Structural Study Optimization

  2020cites this paper
• Dissimilation of synonymous codon usage bias in virus–host coevolution due to translational selection

  2020cites this paper
• Development of a Biosensor for Crotonobetaine-CoA-Ligase Screening based on Elucidation of the Escherichia coli Carnitine Metabolism.

  2020cites this paper
• Developing Aspergillus niger as a cell factory for food enzyme production.

  2020cites this paper
• Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments

  2020cites this paper
• Nature and Regulation of Protein Folding on the Ribosome

  2019cites this paper
• Applications of Protein Engineering and Directed Evolution in Plant Research1[OPEN]

  2019cites this paper
• DNA Chisel, a versatile sequence optimizer

  2019cites this paper
• A New Look at Codon Usage and Protein Expression.

  2019cites this paper
• [Application of codon optimization strategy in heterologous protein expression].

  2019cites this paper
• Improved raw starch amylase production by Saccharomyces cerevisiae using codon optimisation strategies

  2018cites this paper
• Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design

  2018cites this paper