The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.
Plant secretome proteomics
Erik Alexandersson,Ashfaq Ali,S. Resjö,E. Andreasson
Published 2013 in Frontiers in Plant Science
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- Publication year
2013
- Venue
Frontiers in Plant Science
- Publication date
2013-02-01
- Fields of study
Biology, Medicine, Environmental Science
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- External record
- Source metadata
Semantic Scholar, PubMed
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