Functional properties of CYP2D6 1 (wild-type) and CYP2D6 7 (His324Pro) expressed by recombinant baculovirus in insect cells

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Naunyn-Schmiedeberg's Archives of Pharmacology

• Publication date

  1997-02-24

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1007/PL00004948PMID 9089660
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cytochrome P450 nomenclature.

  1998cited by this paper
• Nomenclature for human CYP2D6 alleles.

  1996cited by this paper
• P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature.

  1996cited by this paper
• Functional high level expression of cytochrome P450 CYP2D6 using baculoviral expression systems.

  1996influential reference
• Cytochromes P450 Expression Systems

  1995cited by this paper
• Comparison of substrate metabolism by wild type CYP2D6 protein and a variant containing methionine, not valine, at position 374.

  1995influential reference
• High yield expression of functionally active human liver CYP2D6 in yeast cells.

  1995cited by this paper
• Truncated human P450 2D6: expression in Escherichia coli, Ni(2+)-chelate affinity purification, and characterization of solubility and aggregation.

  1995cited by this paper
• Structure and function of cytochromes P450: a comparative analysis of three crystal structures.

  1995cited by this paper
• CYP3A4 expressed by insect cells infected with a recombinant baculovirus containing both CYP3A4 and human NADPH-cytochrome P450 reductase is catalytically similar to human liver microsomal CYP3A4.

  1995influential reference
• Expression of cytochrome P450 2D6 in Escherichia coli, purification, and spectral and catalytic characterization.

  1995cited by this paper
• "It's the genes, stupid". Molecular bases and clinical consequences of genetic cytochrome P450 2D6 polymorphism.

  1995cited by this paper
• Molecular basis of polymorphic drug metabolism

  1995cited by this paper
• cDNA-directed expression of human cytochrome P450 CYP1A1 using baculovirus. Purification, dependency on NADPH-P450 oxidoreductase, and reconstitution of catalytic properties without purification.

  1995cited by this paper
• An efficient strategy for detection of known and new mutations of the CYP2D6 gene using single strand conformation polymorphism analysis.

  1995cited by this paper
• Cytochromes P450 expression systems.

  1995cited by this paper
• Genetic analysis of the Chinese cytochrome P4502D locus: characterization of variant CYP2D6 genes present in subjects with diminished capacity for debrisoquine hydroxylation.

  1994cited by this paper
• Baculovirus expression of bovine cytochrome P450c17 in Sf9 cells and comparison with expression in yeast, mammalian cells, and E. coli.

  1994cited by this paper
• A missense mutation in exon 6 of the CYP2D6 gene leading to a histidine 324 to proline exchange is associated with the poor metabolizer phenotype of sparteine

  1994cited by this paper
• cDNA-directed expression of human cytochrome P450 CYP3A4 using baculovirus.

  1994cited by this paper
• Comparative recombinant protein production of eight insect cell lines

  1993cited by this paper
• Importance of the proline-rich region following signal-anchor sequence in the formation of correct conformation of microsomal cytochrome P-450s.

  1993cited by this paper
• Evidence for a new variant CYP2D6 allele CYP2D6J in a Japanese population associated with lower in vivo rates of sparteine metabolism.

  1993cited by this paper
• Characterization of a human cell line expressing high levels of cDNA-derived CYP2D6.

  1993cited by this paper
• Baculovirus expression vectors: a laboratory manual.

  1992cited by this paper
• A general and fast method to generate multiple site directed mutations.

  1992cited by this paper
• Catalytic activities of human debrisoquine 4-hydroxylase cytochrome P450 (CYP2D6) expressed in yeast.

  1992cited by this paper
• A predictive model for substrates of cytochrome P450-debrisoquine (2D6).

  1992cited by this paper
• Expression of Three Recombinant Proteins Using Baculovirus Vectors in 23 Insect Cell Lines

  1991cited by this paper
• Identification of a new variant CYP2D6 allele lacking the codon encoding Lys-281: possible association with the poor metabolizer phenotype.

  1991cited by this paper
• Debrisoquine/Sparteine Hydroxylation Genotype and Phenotype: Analysis of Common Mutations and Alleles of CYP2D6 in a European Population

  1991cited by this paper
• Deletion of the entire cytochrome P450 CYP2D6 gene as a cause of impaired drug metabolism in poor metabolizers of the debrisoquine/sparteine polymorphism.

  1991cited by this paper
• Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.

  1990cited by this paper
• Sparteine metabolism capacity in human liver: structural variants of human P450IID6 as assessed by immunochemistry.

  1990influential reference
• Multiple mutations of the human cytochrome P450IID6 gene (CYP2D6) in poor metabolizers of debrisoquine. Study of the functional significance of individual mutations by expression of chimeric genes.

  1990influential reference
• Novel exogenous heme-dependent expression of mammalian cytochrome P450 using baculovirus.

  1989cited by this paper
• The human debrisoquine 4-hydroxylase (CYP2D) locus: sequence and identification of the polymorphic CYP2D6 gene, a related gene, and a pseudogene.

  1989influential reference
• Antibodies: A Laboratory Manual

  1988cited by this paper
• Antibodies against human cytochrome P-450db1 in autoimmune hepatitis type II.

  1988influential reference
• Characterization of the common genetic defect in humans deficient in debrisoquine metabolism

  1988cited by this paper
• Xenobiotic and endobiotic inhibitors of cytochrome P-450dbl function, the target of the debrisoquine/sparteine type polymorphism.

  1988cited by this paper
• Absence of hepatic cytochrome P450bufI causes genetically deficient debrisoquine oxidation in man.

  1988influential reference
• Evidence for altered catalytic properties of the cytochrome P‐450 involved in sparteine oxidation in poor metabolizers

  1987cited by this paper
• Chromosomal assignment of human cytochrome P-450 (debrisoquine/sparteine type) to chromosome 22.

  1987cited by this paper
• High-performance liquid chromatographic assays for bufuralol 1'-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver.

  1987cited by this paper
• A sensitive capillary GC assay for the determination of sparteine oxidation products in microsomal fractions of human liver.

  1986cited by this paper
• Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase.

  1978influential reference
• Some properties of a detergent-solubilized NADPH-cytochrome c(cytochrome P-450) reductase purified by biospecific affinity chromatography.

  1976cited by this paper
• THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

  1964cited by this paper

• Quantitative Proteomics in Translational Absorption, Distribution, Metabolism, and Excretion and Precision Medicine

  2022cites this paper
• Peptic ulcer disease by Helicobacter pylori infection and gastro esophageal reflux disease

  2018cites this paper
• Structure and function of Human CYP2D6

  2018cites this paper
• 10 Pharmacogenomics in Gastroenterology

  2018cites this paper
• Evaluating the impact of missenses mutations in CYP2D6*7 and CYP2D6*14A: does it compromise tamoxifen metabolism?

  2016cites this paper
• Pharmacogenomics in Gastroenterology

  2012cites this paper
• Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study.

  2012cites this paper
• In vitro assessment of the allelic variants of cytochrome P450.

  2012cites this paper
• A new standardized electrochemical array for drug metabolic profiling with human cytochromes P450.

  2011cites this paper
• Enzyme-based amperometric platform to determine the polymorphic response in drug metabolism by cytochromes P450.

  2011cites this paper
• Metabolic activation of phenanthrene by human and mouse cytochromes P450 and pharmacokinetics in CYP1A2 knockout mice.

  2010cites this paper
• Mass spectrometry‐based absolute quantification of microsomal cytochrome P450 2D6 in human liver

  2009cites this paper
• Polymorphism of Human Cytochrome P450 2D6 and Its Clinical Significance

  2009cites this paper
• New insights into the structural characteristics and functional relevance of the human cytochrome P450 2D6 enzyme

  2009cites this paper
• Substrate specificity, inhibitors and regulation of human cytochrome P450 2D6 and implications in drug development.

  2009cites this paper
• Clinical pharmacogenetics and potential application in personalized medicine.

  2008cites this paper
• Biotransformation of the Analgesic-Antipyretic Drugs Metamizole and Aminopyrine by Genetically Polymorphic Enzymes

  2007influential citation
• A Natural Variant of the Heme-Binding Signature (R441C) Resulting in Complete Loss of Function of CYP2D6

  2007cites this paper
• DMD # 15149 1 A Natural Variant of the Heme-Binding Signature ( R 441 C ) Resulting in Complete Loss of Function of CYP 2 D 6

  2007cites this paper
• Molecular analysis of mechanisms leading to CYP2D6 intermediate and ultrarapid metabolizer phenotypes

  2006cites this paper
• Dominant contribution of P450 3A4 to the hepatic carcinogenic activation of aflatoxin B1.

  2006cites this paper
• A silent mutation (2939G>A, exon 6; CYP2D6*59) leading to impaired expression and function of CYP2D6

  2006cites this paper
• METABOLISM OF ALFENTANIL BY CYTOCHROME P4503A (CYP3A) ENZYMES

  2005cites this paper
• Genetic variability of CYP2B6 in populations of African and Asian origin: allele frequencies, novel functional variants, and possible implications for anti-HIV therapy with efavirenz

  2005cites this paper
• Functional analysis of CYP2D6.31 variant: Homology modeling suggests possible disruption of redox partner interaction by Arg440His substitution

  2005cites this paper
• Bioactivation of the Pulmonary Toxicants Naphthalene and 1-Nitronaphthalene by Rat CYP2F4

  2005cites this paper
• Contribution of CYP3A5 to the in vitro hepatic clearance of tacrolimus.

  2005cites this paper
• Polymorphic Cytochrome P450 2D6: Humanized Mouse Model and Endogenous Substrates

  2004cites this paper
• Metabolism of alfentanil by cytochrome P 4503 A ( CYP 3 A ) enzymes

  2004cites this paper
• Limited contribution of CYP3A5 to the hepatic 6β-hydroxylation of testosterone

  2004cites this paper
• Cytochrome P450 2D6: overview and update on pharmacology, genetics, biochemistry

  2003cites this paper
• V79 Chinese hamster cells genetically engineered for polymorphic cytochrome P450 2D6 and their predictive value for humans.

  2003cites this paper
• COMPARATIVE METABOLIC CAPABILITIES OF CYP 3 A 4 , CYP 3 A 5 , AND CYP 3 A 7

  2002influential citation
• Discriminative quantification of cytochrome P4502D6 and 2D7/8 pseudogene expression by TaqMan real-time reverse transcriptase polymerase chain reaction.

  2002cites this paper
• Comparative metabolic capabilities of CYP3A4, CYP3A5, and CYP3A7.

  2002influential citation
• Expression of active human beta-glucuronidase in Sf9 cells infected with recombinant baculovirus.

  2002cites this paper
• Comprehensive analysis of the genetic factors determining expression and function of hepatic CYP2D6.

  2001cites this paper
• Cellular localization and regional distribution of CYP2D6 mRNA and protein expression in human brain.

  2001cites this paper
• Identification of the human cytochromes P450 involved in the oxidative metabolism of "Ecstasy"-related designer drugs.

  2000cites this paper
• Expression und Funktion arzneimittelmetabolisierender Enzyme und des ABC-Transporters P-Glykoprotein in Dünndarm und Leber des Menschen.

  2000cites this paper
• Involvement of human cytochrome P450 2D6 in the bioactivation of acetaminophen.

  2000cites this paper
• Overlapping but distinct specificities of anti‐liver‐kidney microsome antibodies in autoimmune hepatitis type II and hepatitis C revealed by recombinant native CYP2D6 and novel peptide epitopes

  1999cites this paper
• Genetic polymorphisms of human N-acetyltransferase, cytochrome P450, glutathione-S-transferase, and epoxide hydrolase enzymes: relevance to xenobiotic metabolism and toxicity.

  1999cites this paper
• Functional co-expression of CYP2D6 and human NADPH-cytochrome P450 reductase in Escherichia coli.

  1998cites this paper
• Expression and characterization of dog CYP2D15 using baculovirus expression system.

  1998cites this paper
• Molecular mechanisms of genetic polymorphisms of drug metabolism.

  1997cites this paper