Frequency of regulatory B cells in adipose tissue and peripheral blood from individuals with overweight, obesity and normal-weight.

STATEMENTS OF THE PROBLEM Regulatory B (Breg) cells have a critical role in adipose tissue homeostasis, and although subtypes of Breg cells have been described, their contribution during obesity is unclear. Therefore, the levels of regulatory B cells in adipose tissue and peripheral blood samples drawn from individuals with overweight, obesity, and normal-weight were evaluated. METHODS The percentages of Breg cells were analyzed by flow cytometry. The activity of Breg cells was assessed by measuring the release of IFN-γ in the supernatants of co-cultures of CD4+ T and regulatory B cells with an ELISA assay. The levels of IL-17 and IFN-γ produced by the CD4+ T cells were assessed using an ELISA assay. RESULTS Diminished frequencies of Breg cells with phenotypes CD19+CD27+CD38High, CD19+CD24HighCD38High, and CD19+CD24HighCD38HighIL-10+ cells were observed in the blood samples from the individuals with overweight and obesity but not in the individuals with normal-weight. The production of IFN-γ in CD4+ T-cell cultures showed a decrease in the presence of Breg cells in individuals with obesity and normal-weight. We found fewer percentages of CD19+CD27+CD38High cells in the adipose tissue samples from individuals with overweight and obesity compared to individuals with normal-weight. In addition, elevated levels of IL-17 and IFN-γ in the supernatants of the cultures of CD4+ T cells from the individuals with obesity compared to the individuals with normal-weight were observed. CONCLUSIONS The results suggest that individuals with obesity show increased levels of Th1/Th17 cytokines, which might be caused by the decreased frequency of regulatory B cells.

• Publication year

  2018

• Venue

  Obesity Research and Clinical Practice

• Publication date

  2018-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.orcp.2018.07.001PMID 30115554
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• CD19loCD27hi Plasmablasts Suppress Harmful Th17 Inflammation Through Interleukin 10 Pathway in Colorectal Cancer

  2017cited by this paper
• Diminished levels of regulatory T cell subsets (CD8+Foxp3, CD4+Foxp3 and CD4+CD39+Foxp3) but increased Foxp3 expression in adipose tissue from overweight subjects.

  2016cited by this paper
• The complex immunological and inflammatory network of adipose tissue in obesity.

  2016cited by this paper
• Elevated B Cell Activation is Associated with Type 2 Diabetes Development in Obese Subjects

  2016cited by this paper
• Regulatory B cells: origin, phenotype, and function.

  2015cited by this paper
• Regulatory functions of B cells in allergic diseases

  2014cited by this paper
• Interleukin 10 (IL-10)-producing CD1dhi regulatory B cells from Schistosoma haematobium-infected individuals induce IL-10-positive T cells and suppress effector T-cell cytokines.

  2014cited by this paper
• The impact of adiposity on adipose tissue-resident lymphocyte activation in humans

  2014cited by this paper
• B cells promote inflammation in obesity and type 2 diabetes through regulation of T-cell function and an inflammatory cytokine profile

  2013cited by this paper
• Adipose Natural Regulatory B Cells Negatively Control Adipose Tissue Inflammation.

  2013cited by this paper
• CD19+CD24hiCD38hi B Cells Maintain Regulatory T Cells While Limiting TH1 and TH17 Differentiation

  2013cited by this paper
• Obesity prevalence in Mexico: impact on health and economic burden

  2013cited by this paper
• Obese adipocytes show ultrastructural features of stressed cells and die of pyroptosis

  2013cited by this paper
• Interleukin-10 produced by B cells is crucial for the suppression of Th17/Th1 responses, induction of T regulatory type 1 cells and reduction of collagen-induced arthritis

  2012cited by this paper
• Human CD19(+)CD25(high) B regulatory cells suppress proliferation of CD4(+) T cells and enhance Foxp3 and CTLA-4 expression in T-regulatory cells.

  2012cited by this paper
• Lymphocytes in obesity-related adipose tissue inflammation

  2012cited by this paper
• A tolerogenic role for Toll-like receptor 9 is revealed by B-cell interaction with DNA complexes expressed on apoptotic cells

  2011cited by this paper
• Mice Lacking Endogenous IL-10–Producing Regulatory B Cells Develop Exacerbated Disease and Present with an Increased Frequency of Th1/Th17 but a Decrease in Regulatory T Cells

  2011cited by this paper
• Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.

  2011cited by this paper
• Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model.

  2010cited by this paper
• Presence of Foxp3-expressing CD19(+)CD5(+) B Cells in Human Peripheral Blood Mononuclear Cells: Human CD19(+)CD5(+)Foxp3(+) Regulatory B Cell (Breg)

  2010cited by this paper
• IL‐10 produced by activated human B cells regulates CD4+ T‐cell activation in vitro

  2010cited by this paper
• Regulatory B Cells (B10 Cells) Have a Suppressive Role in Murine Lupus: CD19 and B10 Cell Deficiency Exacerbates Systemic Autoimmunity

  2010cited by this paper
• CD19(+)CD24(hi)CD38(hi) B cells exhibit regulatory capacity in healthy individuals but are functionally impaired in systemic Lupus Erythematosus patients.

  2010cited by this paper
• Unexpected trafficking of immune cells within the adipose tissue during the onset of obesity.

  2009cited by this paper
• Lean, but not obese, fat is enriched for a unique population of regulatory T cells that affect metabolic parameters

  2009cited by this paper
• Ablation of CD11c-positive cells normalizes insulin sensitivity in obese insulin resistant animals.

  2008cited by this paper
• Obesity induces a phenotypic switch in adipose tissue macrophage polarization.

  2007cited by this paper
• MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity.

  2006cited by this paper
• Adipocyte death defines macrophage localization and function in adipose tissue of obese mice and humans Published, JLR Papers in Press, September 8, 2005. DOI 10.1194/jlr.M500294-JLR200

  2005cited by this paper
• Prevention of Arthritis by Interleukin 10–producing B Cells

  2003cited by this paper
• This information is current as Development of Memory CD 4 Cells A Critical Role for B Cells in the

  2000cited by this paper

• Interleukin-10 expressing B lineage cells in visceral adipose tissue protect against aging-related insulin resistance and extend lifespan.

  2026cites this paper
• The immune tolerance role of Bregs in inhibiting human inflammatory diseases, with a focus on diabetes mellitus

  2025cites this paper
• Expression of the Transcription Factor FOXP3 in Human Peripheral Blood B-Cell Subtypes (CD19+CD39+ and CD19+CD39-) and Evaluation of Their Regulatory Function

  2025cites this paper
• Obese individuals exhibit distinct temporal gene expression dynamics in response to influenza vaccination

  2025cites this paper
• Neuro-immune Interactions in Metabolic Regulation: Brain and Adipose Tissue Crosstalk

  2025cites this paper
• Regulatory B Cells—Immunopathological and Prognostic Potential in Humans

  2024cites this paper
• Fat‐associated lymphoid clusters: Supporting visceral adipose tissue B cell function in immunity and metabolism

  2024cites this paper
• The Role of Regulatory B Lymphocytes in Allergic Diseases

  2024cites this paper
• Exploring the Link between Inflammatory Biomarkers and Adipometrics in Healthy Young Adults Aged 20–35 Years

  2024cites this paper
• Effect of Per and Poly-Fluoroalkyl Substances on Pregnancy and Child Development.

  2024cites this paper
• Adipose tissue lymphocytes and obesity

  2023cites this paper
• Age-Dependent Effect of Calcitriol on Mouse Regulatory T and B Lymphocytes

  2023cites this paper
• Regulatorische B-Zellen – immunpathologisches und prognostisches Potenzial beim Menschen

  2023cites this paper
• Mechanism of immune attack in the progression of obesity-related type 2 diabetes

  2023cites this paper
• Distinct B cell profiles characterise healthy weight and obesity pre- and post-bariatric surgery

  2023cites this paper
• Supplementation with EPA and DHA omega-3 fatty acids improves peripheral immune cell mitochondrial dysfunction and inflammation in subjects with obesity.

  2023cites this paper
• Lipid metabolism in regulation of B cell development and autoimmunity.

  2023cites this paper
• Inflammaging and body composition: New insights in diabetic and hypertensive elderly men.

  2022cites this paper
• Helminth and Host Crosstalk: New Insight Into Treatment of Obesity and Its Associated Metabolic Syndromes

  2022cites this paper
• Impact of Obesity on Vaccination to SARS-CoV-2

  2022cites this paper
• Effectiveness of SARS-CoV-2 Vaccines for Short- and Long-Term Immunity: A General Overview for the Pandemic Contrast

  2022cites this paper
• The Role of B Lymphocyte Subsets in Adipose Tissue Development, Metabolism, and Aging.

  2022cites this paper
• B cell contribution to immunometabolic dysfunction and impaired immune responses in obesity

  2022cites this paper
• Use of the NIH consensus criteria in cellular and soluble biomarker research in chronic graft-versus-host disease: A systematic review

  2022cites this paper
• The role of immune dysfunction in obesity-associated cancer risk, progression, and metastasis

  2021cites this paper
• Adipose tissue immune cells in obesity, type 2 diabetes mellitus and cardiovascular diseases.

  2021cites this paper
• Implications of Inflammatory States on Dysfunctional Immune Responses in Aging and Obesity

  2021cites this paper
• Early Onset of SARS-COV-2 Antibodies after First Dose of BNT162b2: Correlation with Age, Gender and BMI

  2021cites this paper
• The emerging field of regulatory B cell immunometabolism.

  2021cites this paper
• Immunosuppressive Mechanisms of Regulatory B Cells

  2021cites this paper
• Immunometabolism in type 2 diabetes mellitus: tissue-specific interactions

  2020influential citation
• Adipose Tissue Immunomodulation: A Novel Therapeutic Approach in Cardiovascular and Metabolic Diseases

  2020cites this paper
• Obesity Accelerates Age Defects in Mouse and Human B Cells

  2020cites this paper
• ATVB IN FOCUS: Adipose Tissue and Vascular and Immune Cell Cross Talk: Implications for Obesity and Vascular Disease

  2020cites this paper
• Inflammaging as a common ground for the development and maintenance of sarcopenia, obesity, cardiomyopathy and dysbiosis.

  2019cites this paper
• An update on immune dysregulation in obesity‐related insulin resistance

  2019cites this paper