3D Functional Ultrasound Imaging of Pigeons

Recent advances in ultrasound Doppler imaging have allowed to visualize brain activity in small mammalian species such as rats and mice. In birds, this type of functional ultrasound imaging was impossible up to now because birds have physiological characteristics that are unfavorable for current functional ultrasound acquisition schemes. Here, we introduce a high-definition functional ultrasound acquisition method (HDfUS) acquiring 20,000 frames per second continuously. This enabled first successful functional studies on awake pigeons subjected to auditory and visual stimulation. We show that the improved spatiotemporal resolution and sensitivity of HDfUS allows to visualize and investigate the temporally resolved 3D neural activity evoked by a complex stimulation pattern, such as a moving light source. This illustrates the enormous potential of HDfUS imaging to become a new standard functional brain imaging method revealing unknown, stimulus related hemodynamics at excellent signal-to-noise ratio and spatiotemporal resolution. Highlights - We describe a novel ultrafast functional ultrasound technique (HDfUS) - HDfUS offers continuous recording with unmatched spatiotemporal resolution - HDfUS allows to resolve complex 4D neurovascular responses in the brain - First fUS study on non-mammalian species

• Publication year

  2018

• Venue

  bioRxiv

• Publication date

  2018-04-18

• Fields of study

  Biology, Medicine, Computer Science, Engineering

• Identifiers
  DOI 10.1016/j.neuroimage.2018.08.014PMID 30118869
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Light controls cerebral blood flow in naive animals

  2017cited by this paper
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  2017cited by this paper
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  2017cited by this paper
• 3D functional ultrasound imaging of the cerebral visual system in rodents

  2017cited by this paper
• fMRI mapping of the visual system in the mouse brain with interleaved snapshot GE-EPI

  2016cited by this paper
• Asymmetric top-down modulation of ascending visual pathways in pigeons.

  2016cited by this paper
• 4D microvascular imaging based on ultrafast Doppler tomography

  2016cited by this paper
• Spatiotemporal Clutter Filtering of Ultrafast Ultrasound Data Highly Increases Doppler and fUltrasound Sensitivity

  2015influential reference
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  2015influential reference
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  2015cited by this paper
• Comparison of stimulus-evoked cerebral hemodynamics in the awake mouse and under a novel anesthetic regime

  2015cited by this paper
• A role for descending auditory cortical projections in songbird vocal learning

  2014cited by this paper
• Functional ultrasound imaging of intrinsic connectivity in the living rat brain with high spatiotemporal resolution

  2014influential reference
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  2014cited by this paper
• Chronic assessment of cerebral hemodynamics during rat forepaw electrical stimulation using functional ultrasound imaging

  2014cited by this paper
• Ultrafast imaging in biomedical ultrasound

  2014cited by this paper
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  2013cited by this paper
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  2013cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2009cited by this paper
• What we can do and what we cannot do with fMRI

  2008cited by this paper
• Functional MRI of the zebra finch brain during song stimulation suggests a lateralized response topography

  2007cited by this paper
• Neural mechanisms of birdsong memory

  2006cited by this paper
• Current Techniques in Avian Anesthesia

  2005influential reference
• Bilateral brain processes for comprehending natural language.

  2005cited by this paper
• The laboratory mouse

  2004cited by this paper
• Early language acquisition: cracking the speech code

  2004cited by this paper
• Learned Birdsong and the Neurobiology of Human Language

  2004cited by this paper
• Asymmetry pays: visual lateralization improves discrimination success in pigeons.

  2000cited by this paper
• Localized neuronal activation in the zebra finch brain is related to the strength of song learning.

  2000cited by this paper
• Birdsong and human speech: common themes and mechanisms.

  1999cited by this paper
• Estimation of Blood Velocities Using Ultrasound: A Signal Processing Approach

  1996cited by this paper
• Scattering of Ultrasound by Blood

  1976cited by this paper
• A stereotaxic atlas of the brain of the pigeon (Columba livia)

  1967cited by this paper

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• Spatiotemporal Clustering of Functional Ultrasound Signals at the Single-Voxel Level

  2025cites this paper
• Unveiling the potential of ultrasound in brain imaging: Innovations, challenges, and prospects.

  2024cites this paper
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  2024cites this paper
• A deep learning classification task for brain navigation in rodents using micro-Doppler ultrasound imaging

  2024cites this paper
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  2023cites this paper
• Stratum Griseum Periventriculare-mediated Fear Emotion Regulates Motor Behavior in Pigeons

  2023cites this paper
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  2023cites this paper
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  2023cites this paper
• Swept-3-D Ultrasound Imaging of the Mouse Brain Using a Continuously Moving 1-D-Array—Part I: Doppler Imaging

  2023cites this paper
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  2023cites this paper
• GLM-Regularized Low-Rank Factorization For Extracting Functional Response From Swept-3D Functional Ultrasound

  2023cites this paper
• Super-resolution Ultrasound Microvascular Angiography for Spinal Cord Penumbra Imaging.

  2023cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
• Functional Ultrasound Neuroimaging.

  2022cites this paper
• High-resolution micro-Doppler imaging during neurosurgical resection of an arteriovenous malformation: illustrative case

  2022cites this paper
• Progresses of animal robots: A historical review and perspectiveness

  2022cites this paper
• 3D Functional Ultrasound using a Continuously Moving Linear Stage

  2022cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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