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Fluorescence turn on detection of bilirubin using Fe (III) modulated BSA stabilized copper nanocluster; A mechanistic perception.

M. Jayasree,R. Aparna,R. R. Anjana,J. S. Anjali Devi,Nebu John,K. Abha,A. Manikandan,Sony George

Published 2018 in Analytica Chimica Acta

ABSTRACT

Hyperbilirubinemia is the condition when bilirubin exceeds normal concentration in body (19.80 mg/mL in newborns and 1.19 × 10-2 mg/mL in adults). Bilirubin encephalopathy in newborns may cause irreversible neurological disorders. Current methods for detection of bilirubin suffer from compromising accuracy. In the present work, bovine serum albumin stabilized copper nanocluster (BSA-CuNCs) was synthesized via a one pot microwave assisted method as a turn on detector for bilirubin. The synthesized BSA-CuNCs having size less than 4 nm, exhibited bright blue emission at 405 nm. Interestingly, no observable change in fluorescence emission was noticed over a wide pH range (1-11) or at high ionic conditions. However, the interaction of Fe3+ with BSA-CuNCs induces quenching of fluorescence. Moreover, the fluorescence can be regained by the addition of bilirubin over other possible coexisting biomolecules. A good linearity was observed for BSA-CuNCs based turn on probe with a Limit of Detection (LoD) 6.62 nM. Furthermore, real sample analyses were carried out with human serum and urine which showed good recovery percentage.

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