Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line

Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewed as efficiently as those from wild-type (Pax7+/+) mice. Taken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases.

• Publication year

  2018

• Venue

  Skeletal Muscle

• Publication date

  2018-08-24

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1186/s13395-018-0174-xPMID 30139390PMCID 6108100
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Induction of muscle stem cell quiescence by the secreted niche factor Oncostatin M

  2018cited by this paper
• PAX7 Targets, CD54, Integrin α9β1, and SDC2, Allow Isolation of Human ESC/iPSC-Derived Myogenic Progenitors.

  2017cited by this paper
• PAX7 target genes are globally repressed in facioscapulohumeral muscular dystrophy skeletal muscle

  2017cited by this paper
• Visualizing the Functional Heterogeneity of Muscle Stem Cells.

  2016cited by this paper
• Notch Signaling Rescues Loss of Satellite Cells Lacking Pax7 and Promotes Brown Adipogenic Differentiation.

  2016cited by this paper
• Satellite Cell Heterogeneity in Skeletal Muscle Homeostasis.

  2016cited by this paper
• Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting

  2016cited by this paper
• The central role of muscle stem cells in regenerative failure with aging

  2015cited by this paper
• Muscle stem cell fate is controlled by the cell-polarity protein Scrib.

  2015cited by this paper
• Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting

  2015cited by this paper
• Satellite cell heterogeneity and hierarchy in skeletal muscle

  2014cited by this paper
• Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells.

  2013cited by this paper
• Satellite cells and the muscle stem cell niche.

  2013cited by this paper
• Pax7 is critical for the normal function of satellite cells in adult skeletal muscle

  2013cited by this paper
• NF-κB-mediated Pax7 dysregulation in the muscle microenvironment promotes cancer cachexia.

  2013cited by this paper
• Satellite cells are essential for skeletal muscle regeneration: the cell on the edge returns centre stage

  2012cited by this paper
• Pax7 Lineage Contributions to the Mammalian Neural Crest

  2012cited by this paper
• A subpopulation of adult skeletal muscle stem cells retains all template DNA strands after cell division.

  2012cited by this paper
• Tissue-specific stem cells: lessons from the skeletal muscle satellite cell.

  2012cited by this paper
• Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle

  2012cited by this paper
• Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle

  2012cited by this paper
• An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration

  2011cited by this paper
• BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells

  2011cited by this paper
• Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration

  2011cited by this paper
• Effective fiber hypertrophy in satellite cell-depleted skeletal muscle

  2011cited by this paper
• Inducible lineage tracing of Pax7‐descendant cells reveals embryonic origin of adult satellite cells

  2010cited by this paper
• Muscle satellite cells are a functionally heterogeneous population in both somite-derived and branchiomeric muscles

  2010cited by this paper
• Short telomeres and stem cell exhaustion model Duchenne muscular dystrophy in mdx/mTR mice.

  2010cited by this paper
• Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements

  2009cited by this paper
• Mature Adult Dystrophic Mouse Muscle Environment Does Not Impede Efficient Engrafted Satellite Cell Regeneration and Self‐Renewal

  2009cited by this paper
• Prospective Isolation of Skeletal Muscle Stem Cells with a Pax7 Reporter

  2008cited by this paper
• Self-renewal and expansion of single transplanted muscle stem cells

  2008cited by this paper
• Molecular Signature of Quiescent Satellite Cells in Adult Skeletal Muscle

  2007cited by this paper
• Pax7 and myogenic progression in skeletal muscle satellite cells

  2006cited by this paper
• Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis

  2006cited by this paper
• Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells

  2006cited by this paper
• A Pax3/Pax7-dependent population of skeletal muscle progenitor cells

  2005cited by this paper
• Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche.

  2005cited by this paper
• Direct Isolation of Satellite Cells for Skeletal Muscle Regeneration

  2005cited by this paper
• Divergent functions of murine Pax3 and Pax7 in limb muscle development.

  2004cited by this paper
• Pax7 directs postnatal renewal and propagation of myogenic satellite cells but not their specification

  2004cited by this paper
• Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal

  2004cited by this paper
• Isolation of adult mouse myogenic progenitors: functional heterogeneity of cells within and engrafting skeletal muscle.

  2004cited by this paper
• Muscle satellite cells adopt divergent fates

  2004cited by this paper
• Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal.

  2004cited by this paper
• Pax7 is required for the specification of myogenic satellite cells.

  2000cited by this paper
• SATELLITE CELL OF SKELETAL MUSCLE FIBERS

  1961cited by this paper
• Satellite cells , connective tissue fibroblasts and their interactions are crucial for muscle regeneration

  year unknowncited by this paper

• Therapeutic strategies targeting muscle stem cells in satellite cell-opathies.

  2026cites this paper
• WDR62 is required for proper proliferation and early differentiation of skeletal myoblasts

  2026cites this paper
• Nuclear Deformities Minimally Affect Fiber‐Type‐Specific Disease Progression in Murine Models of Nuclear Envelope Myopathy

  2025cites this paper
• Hibernation as a model for skeletal muscle preservation

  2025cites this paper
• Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation

  2024cites this paper
• Loss of Tob1 promotes muscle regeneration through muscle stem cell expansion.

  2024cites this paper
• Fibroblast growth factor 8b (FGF-8b) enhances myogenesis and inhibits adipogenesis in rotator cuff muscle cell populations in vitro

  2023cites this paper
• Generation of a MyoD knock-in reporter mouse line to study muscle stem cell dynamics and heterogeneity

  2023cites this paper
• Pax7 reporter mouse models: a pocket guide for satellite cell research

  2023influential citation
• The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration

  2022cites this paper
• Transplantation to study satellite cell heterogeneity in skeletal muscle

  2022cites this paper
• Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells

  2021cites this paper
• RNU (Foxn1RNU-Nude) Rats Demonstrate an Improved Ability to Regenerate Muscle in a Volumetric Muscle Injury Compared to Sprague Dawley Rats

  2021cites this paper
• The mechanosensitive Ca2+-permeable ion channel PIEZO1 promotes satellite cell function in skeletal muscle regeneration

  2021cites this paper
• SMART approaches for genome-wide analyses of skeletal muscle stem and niche cells

  2021cites this paper
• Understanding the biology of volumetric muscle loss for an individualized exercise rehabilitation approach in breast cancer patients.

  2021cites this paper
• A Modified Pre-plating Method for High-Yield and High-Purity Muscle Stem Cell Isolation From Human/Mouse Skeletal Muscle Tissues

  2020cites this paper
• Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

  2020cites this paper
• Damaged Myofiber-Derived Metabolic Enzymes Act as Activators of Muscle Satellite Cells

  2020cites this paper
• Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle

  2020influential citation
• The body region specificity in murine models of muscle regeneration and atrophy

  2020cites this paper
• Inducible Rpt3, a Proteasome Component, Knockout in Adult Skeletal Muscle Results in Muscle Atrophy

  2020cites this paper
• Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice

  2019cites this paper
• The Ubiquitin-Proteasome System Is Indispensable for the Maintenance of Muscle Stem Cells

  2018cites this paper
• mecha-The neuronal nitric oxide synthase expression increases during satellite cell-derived primary myoblasts differentiation

  year unknowncites this paper