Increased chromosomal mobility after DNA damage is controlled by interactions between the recombination machinery and the checkpoint

In this study, Smith et al. investigated how cells modulate chromosome mobility in response to DNA damage. They show that global chromosome mobility is regulated by the Rad51 recombinase and its mediator, Rad52, and their findings indicate that interplay between recombination factors and the checkpoint restricts increased mobility until recombination proteins are assembled at damaged sites.

• Publication year

  2018

• Venue

  Genes & Development

• Publication date

  2018-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1101/gad.317966.118PMID 30181361PMCID 6120718
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Chromatin organization and dynamics in double-strand break repair.

  2017cited by this paper
• Nuclear Dynamics of Heterochromatin Repair.

  2017cited by this paper
• Histone degradation in response to DNA damage enhances chromatin dynamics and recombination rates

  2017cited by this paper
• Chromatin stiffening underlies enhanced locus mobility after DNA damage in budding yeast

  2017cited by this paper
• Visualization of Chromatin Decompaction and Break Site Extrusion as Predicted by Statistical Polymer Modeling of Single-Locus Trajectories.

  2017cited by this paper
• Poetry in motion: Increased chromosomal mobility after DNA damage.

  2017cited by this paper
• Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage

  2017cited by this paper
• Quantitative cell biology: uniting disciplines to understand the cell

  2017cited by this paper
• Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage

  2017cited by this paper
• DNA damage signalling targets the kinetochore to promote chromatin mobility

  2016influential reference
• Temporal and Spatial Uncoupling of DNA Double Strand Break Repair Pathways within Mammalian Heterochromatin.

  2016cited by this paper
• A Synthetic Dosage Lethal Genetic Interaction Between CKS1B and PLK1 Is Conserved in Yeast and Human Cancer Cells

  2016cited by this paper
• Evidence for a dual role of actin in regulating chromosome organization and dynamics in yeast

  2016cited by this paper
• 53BP1 and the LINC Complex Promote Microtubule-Dependent DSB Mobility and DNA Repair.

  2015cited by this paper
• Interchromosomal homology searches drive directional ALT telomere movement and synapsis.

  2014cited by this paper
• Protein dynamics during presynaptic complex assembly on individual ssDNA molecules

  2014cited by this paper
• A role for nuclear envelope–bridging complexes in homology-directed repair

  2014cited by this paper
• Mechanisms and Regulation of Mitotic Recombination in Saccharomyces cerevisiae

  2014cited by this paper
• Interplays between ATM/Tel1 and ATR/Mec1 in sensing and signaling DNA double-strand breaks.

  2013cited by this paper
• Cohesin and the nucleolus constrain the mobility of spontaneous repair foci

  2013cited by this paper
• DNA in motion during double-strand break repair.

  2013cited by this paper
• Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage

  2013influential reference
• Rad51 Is an Accessory Factor for Dmc1-Mediated Joint Molecule Formation During Meiosis

  2012cited by this paper
• Increased chromosome mobility facilitates homology search during recombination

  2012cited by this paper
• Increased mobility of double-strand breaks requires Mec1, Rad9 and the homologous recombination machinery

  2012influential reference
• Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair.

  2011cited by this paper
• Regulation of Rad51 function by phosphorylation

  2011cited by this paper
• Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium.

  2010cited by this paper
• Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo

  2009cited by this paper
• Rad 52 recruitment is DNA replication independent and regulated by Cdc 28 and the Mec 1 kinase

  2009cited by this paper
• Rad52 recruitment is DNA replication independent and regulated by Cdc28 and the Mec1 kinase

  2009cited by this paper
• Dynamic chromosome movements during meiosis: a way to eliminate unwanted connections?

  2009cited by this paper
• Colocalization of sensors is sufficient to activate the DNA damage checkpoint in the absence of damage.

  2008influential reference
• 53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility

  2008cited by this paper
• Apn1 and Apn2 endonucleases prevent accumulation of repair-associated DNA breaks in budding yeast as revealed by direct chromosomal analysis

  2008cited by this paper
• The organization of the core proteins of the yeast spindle pole body.

  2005cited by this paper
• Dynamics of DNA Double-Strand Breaks Revealed by Clustering of Damaged Chromosome Domains

  2004influential reference
• Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins.

  2004cited by this paper
• Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre

  2003cited by this paper
• Yeast Rad52 and Rad51 Recombination Proteins Define a Second Pathway of DNA Damage Assessment in Response to a Single Double-Strand Break

  2003cited by this paper
• Interaction with Rad51 Is Indispensable for Recombination Mediator Function of Rad52*

  2002cited by this paper
• Rad52 Protein Associates with Replication Protein A (RPA)-Single-stranded DNA to Accelerate Rad51-mediated Displacement of RPA and Presynaptic Complex Formation*

  2002cited by this paper
• Cloning-free genome alterations in Saccharomyces cerevisiae using adaptamer-mediated PCR.

  2002cited by this paper
• An ATR- and Chk1-Dependent S Checkpoint Inhibits Replicon Initiation following UVC-Induced DNA Damage

  2002cited by this paper
• Two checkpoint complexes are independently recruited to sites of DNA damage in vivo.

  2001cited by this paper
• How do meiotic chromosomes meet their homologous partners?: lessons from fission yeast

  2001cited by this paper
• Chromosome Dynamics in the Yeast Interphase Nucleus

  2001cited by this paper
• From the Cover: Rad52 forms DNA repair and recombination centers during S phase

  2001cited by this paper
• ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK

  1999cited by this paper
• A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools.

  1998cited by this paper
• Interphase chromosomes undergo constrained diffusional motion in living cells.

  1997cited by this paper
• Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways.

  1996influential reference
• Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination.

  1994cited by this paper
• The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene.

  1989cited by this paper
• Repair in Schizosaccharomyces pombe as measured by recovery from caffeine enhancement of radiation-induced lethality

  1975cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• On a Test of Whether one of Two Random Variables is Stochastically Larger than the Other

  1947cited by this paper

• The Role of Transient Crosslinks in the Chromatin Search Response to DNA Damage

  2025cites this paper
• RAD51 regulates eukaryotic chromatin motions in the absence of DNA damage

  2024cites this paper
• Closing the loops: chromatin loop dynamics after DNA damage

  2024cites this paper
• The SMC5/6 complex: folding chromosomes back into shape when genomes take a break

  2024cites this paper
• Distinct mobility patterns of BRCA2 molecules at DNA damage sites

  2023cites this paper
• Plant chromatin on the move: an overview of chromatin mobility during transcription and DNA repair.

  2023cites this paper
• Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generation

  2022cites this paper
• The Dynamic Behavior of Chromatin in Response to DNA Double-Strand Breaks

  2022influential citation
• Temporal coordination between chromosome mobility and homologous recombination

  2022cites this paper
• Global chromatin mobility induced by a DSB is dictated by chromosomal conformation and defines the HR outcome

  2022influential citation
• Measuring Chromosome Pairing During Homologous Recombination in Yeast.

  2021cites this paper
• Modified chromosome structure caused by phosphomimetic H2A modulates the DNA damage response by increasing chromatin mobility in yeast

  2021influential citation
• Damage-induced chromatome dynamics link Ubiquitin ligase and proteasome recruitment to histone loss and efficient DNA repair.

  2021cites this paper
• Chromosome structure due to phospho-mimetic H2A modulates DDR through increased chromatin mobility

  2020cites this paper
• Telomere relocalization to the nuclear pore complex in response to replication stress

  2020cites this paper
• Emerging non-canonical roles for the Rad51–Rad52 interaction in response to double-strand breaks in yeast

  2020cites this paper
• Monitoring global chromatin dynamics in response to DNA damage.

  2020cites this paper
• Complex Chromatin Motions for DNA Repair

  2020cites this paper
• Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore

  2020cites this paper
• Collaborations between chromatin and nuclear architecture to optimize DNA repair fidelity.

  2020influential citation
• In budding yeast and mammals, double strand breaks trigger global chromatin mobility

  2020influential citation
• Assembling nuclear domains: Lessons from DNA repair

  2019influential citation
• Mobility and Repair of Damaged DNA: Random or Directed?

  2019cites this paper
• RAD51 promotes non-homologous genetic rearrangements that are prevented by 53BP1

  2019cites this paper
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  2019cites this paper
• Radiation-induced DNA damage and repair effects on 3D genome organization

  2019cites this paper
• DNA damage triggers increased mobility of chromosomes in G1-phase cells

  2019cites this paper