Comparison of adsorption and conjugation of Herceptin on poly(lactic-co-glycolic acid) nanoparticles - Effect on cell internalization in breast cancer cells.

Surfaces of nanoparticles with are commonly modified to enhance the targeting effect. In this study, we performed surface modifications of docetaxel (DTX)-loaded poly(lactic-co-glycolic acid) nanoparticles (PNPs) with Herceptin® (HCT) to improve the internalization and cytotoxicity in breast cancer cells. The PNPs were prepared with surfactant, poly(ethylene-alt-maleic anhydride) (PEMA), including a number of carboxyl groups for conjugation. Three types of PNPs were prepared via different methods such as adsorption, charged adsorption, and bio-conjugation. The PNPs were evaluated in terms of physical properties, stability, cellular uptake and cytotoxicity. The docetaxel-loaded PNPs with HCT were successfully surface-modified with mean particle sizes of 338.4 ± 59.8 nm (DTX-PNPs), 353.9 ± 67.5 nm (HCT-A-DTX-PNPs), 544.8 ± 301.7 nm (HCT-C-DTX-PNPs), and 499.1 ± 71.9 nm (HCT-B-DTX-PNPs). Cellular uptake of HCT-B-PNPs was 5.0-, 4.4-, and 4.6-fold higher than that of PNPs in BT-474, SK-BR-3, and MCF-7 cells, respectively, at 2 h. At 40 μg/mL, HCT-B-DTX-PNPs showed a higher cytotoxicity toward BT-474, SK-BR-3, and MCF-7 cells than the other formulations. In conclusion, HCT-B-DTX-PNPs were found to possess a higher affinity for breast cancer cells and induce a stronger cytotoxicity than that of other PNPs.

• Publication year

  2018

• Venue

  Materials Science and Engineering C: Materials for Biological Applications

• Publication date

  2018-11-01

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1016/j.msec.2018.06.059PMID 30184775
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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