Development and evaluation of monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for the diagnosis of acute leptospirosis in humans.

Monoclonal antibody (MAb)-based ELISA was developed to detect leptospiral antigens from the plasma of febrile patients. The MAb reacts specifically with pathogenic leptospires and the assay possesses excellent diagnostic test parameters compared to PCR, indicating that this new ELISA is useful for the early diagnosis of leptospirosis with low operational cost.

• Publication year

  2018

• Venue

  JIM - Journal of Immunological Methods

• Publication date

  2018-12-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1016/j.jim.2018.09.006PMID 30217719
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents

  2015cited by this paper
• Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

  2013cited by this paper
• Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.

  2013cited by this paper
• BMC Infectious Diseases BioMed Central Research article Using high titer West Nile intravenous immunoglobulin from selected Israeli donors for treatment of West Nile virus infection

  2009cited by this paper
• Application of Monoclonal Antibodies in a Rapid Sandwich Dot-Enzyme Linked Immunosorbent Assay for Identification and Antigen Detection of Leptospira Serovars

  2008cited by this paper
• Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture.

  2008cited by this paper
• In LipL32, the Major Leptospiral Lipoprotein, the C Terminus Is the Primary Immunogenic Domain and Mediates Interaction with Collagen IV and Plasma Fibronectin

  2008cited by this paper
• Early diagnosis of human leptospirosis by antigen detection in blood.

  2006cited by this paper

• Performance of electrochemical aptasensor as antigen test in clinical samples for early diagnosis of leptospirosis

  2025cites this paper
• Diagnosis of leptospirosis in animals: challenges and perspectives

  2025cites this paper
• Integrated Lateral Flow Electrochemical Strip for Leptospirosis Diagnosis.

  2022cites this paper
• Development and evaluation of polyclonal antibody-based antigen detection ELISA and dot blot assays as a less costly diagnostic alternative for pathogenic leptospira infection.

  2022cites this paper
• Leptospirosis as an unusual culprit of acute pancreatitis and portal vein thrombosis in a New Yorker

  2020cites this paper
• Effective Use of PCR of Cerebrospinal Fluid for Diagnosis of Acute Phase Leptospirosis：A Case Report

  2020cites this paper