Sobremesa L-type Amino Acid Transporter Expressed in Glia Is Essential for Proper Timing of Development and Brain Growth

Summary In Drosophila, ecdysone hormone levels determine the timing of larval development. Its production is regulated by the stereotypical rise in prothoracicotropic hormone (PTTH) levels. Additionally, ecdysone levels can also be modulated by nutrition (specifically by amino acids) through their action on Drosophila insulin-like peptides (Dilps). Moreover, in glia, amino-acid-sensitive production of Dilps regulates brain development. In this work, we describe the function of an SLC7 amino acid transporter, Sobremesa (Sbm). Larvae with reduced Sbm levels in glia remain in third instar for an additional 24 hr. These larvae show reduced brain growth with increased body size but do not show reduction in insulin signaling or production. Interestingly, Sbm downregulation in glia leads to reduced Ecdysone production and a surprising delay in the rise of PTTH levels. Our work highlights Sbm as a modulator of both brain development and the timing of larval development via an amino-acid-sensitive and Dilp-independent function of glia.

• Publication year

  2018

• Venue

  Cell Reports

• Publication date

  2018-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.celrep.2018.08.067PMID 30231999PMCID 6167638
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Prothoracicotropic hormone modulates environmental adaptive plasticity through the control of developmental timing

  2018cited by this paper
• JhI-21 plays a role in Drosophila insulin-like peptide release from larval IPCs via leucine transport

  2018cited by this paper
• Spatio-temporal pattern of neuronal differentiation in the Drosophila visual system: A user's guide to the dynamic morphology of the developing optic lobe.

  2017cited by this paper
• The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology, and Influences Locomotor Activity in Drosophila Larvae

  2016cited by this paper
• Drosophila insulin release is triggered by adipose Stunted ligand to brain Methuselah receptor

  2016cited by this paper
• Signaling from Glia and Cholinergic Neurons Controls Nutrient-Dependent Production of an Insulin-like Peptide for Drosophila Body Growth.

  2015influential reference
• Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells

  2015cited by this paper
• Sensing of amino acids in a dopaminergic circuitry promotes rejection of an incomplete diet in Drosophila.

  2014influential reference
• Methods for studying metabolism in Drosophila.

  2014cited by this paper
• The Drosophila insulin-degrading enzyme restricts growth by modulating the PI3K pathway in a cell-autonomous manner

  2014cited by this paper
• The developmental control of size in insects

  2014cited by this paper
• Gap Junction Proteins in the Blood-Brain Barrier Control Nutrient-Dependent Reactivation of Drosophila Neural Stem Cells

  2014influential reference
• A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila

  2013cited by this paper
• Protection of Neuronal Diversity at the Expense of Neuronal Numbers during Nutrient Restriction in the Drosophila Visual System

  2013cited by this paper
• Neuroendocrine Control of Drosophila Larval Light Preference

  2013cited by this paper
• The SLC3 and SLC7 families of amino acid transporters.

  2013influential reference
• Nerveless and gutsy: intestinal nutrient sensing from invertebrates to humans

  2012cited by this paper
• Direct sensing of systemic and nutritional signals by hematopoietic progenitors in Drosophila

  2012cited by this paper
• Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion.

  2012cited by this paper
• Fat cells reactivate quiescent neuroblasts via TOR and glial Insulin relays in Drosophila

  2011influential reference
• Anaplastic lymphoma kinase spares organ growth during nutrient restriction in Drosophila.

  2011influential reference
• Glial solute carrier transporters in drosophila and mice

  2011cited by this paper
• Refinement of Tools for Targeted Gene Expression in Drosophila

  2010cited by this paper
• The Insect Neuropeptide PTTH Activates Receptor Tyrosine Kinase Torso to Initiate Metamorphosis

  2009cited by this paper
• Ensheathing Glia Function as Phagocytes in the Adult Drosophila Brain

  2009cited by this paper
• Drosophila expresses a CD98 transporter with an evolutionarily conserved structure and amino acid-transport properties.

  2009influential reference
• Remote control of insulin secretion by fat cells in Drosophila.

  2009cited by this paper
• A glial amino-acid transporter controls synapse strength and courtship in Drosophila

  2008cited by this paper
• Organization and Postembryonic Development of Glial Cells in the Adult Central Brain of Drosophila

  2008cited by this paper
• Hemolymph amino acid analysis of individual Drosophila larvae.

  2008cited by this paper
• Nonvesicular Release of Glutamate by Glial xCT Transporters Suppresses Glutamate Receptor Clustering In Vivo

  2007cited by this paper
• Size assessment and growth control: how adult size is determined in insects

  2007cited by this paper
• Prothoracicotropic hormone regulates developmental timing and body size in Drosophila.

  2007influential reference
• microRNA miR-14 acts to modulate a positive autoregulatory loop controlling steroid hormone signaling in Drosophila.

  2007cited by this paper
• How flies get their size: genetics meets physiology

  2006cited by this paper
• The role of the prothoracic gland in determining critical weight for metamorphosis in Drosophila melanogaster.

  2005cited by this paper
• The genetics of heteromeric amino acid transporters.

  2005cited by this paper
• Antagonistic Actions of Ecdysone and Insulins Determine Final Size in Drosophila

  2005cited by this paper
• An axon scaffold induced by retinal axons directs glia to destinations in the Drosophila optic lobe

  2004cited by this paper
• CATs and HATs: the SLC7 family of amino acid transporters

  2004influential reference
• Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors

  2003cited by this paper
• A nutrient sensor mechanism controls Drosophila growth.

  2003influential reference
• Peripheral glia direct axon guidance across the CNS/PNS transition zone.

  2001cited by this paper

• Invertebrate glial barriers as a model for understanding blood–brain barrier evolution

  2025cites this paper
• Genetic Variation in Trophic Avoidance Behaviour Shows Fruit Flies are Generally Attracted to Bacterial Substrates

  2024cites this paper
• A LAT1-Like Amino Acid Transporter Regulates Neuronal Activity in the Drosophila Mushroom Bodies

  2024influential citation
• Genetic variation in trophic avoidance shows fruit flies are generally attracted to bacterial pathogens

  2024cites this paper
• Differential amino acid transporter expression in adult Drosophila melanogaster tissues

  2024cites this paper
• The Drosophila blood-brain barrier emerges as a model for understanding human brain diseases.

  2023cites this paper
• Phytoconstituent Profiles Associated with Relevant Antioxidant Potential and Variable Nutritive Effects of the Olive, Sweet Almond, and Black Mulberry Gemmotherapy Extracts

  2023cites this paper
• Endocrine Regulation of Lifespan in Insect Diapause

  2022cites this paper
• Non-autonomous regulation of neurogenesis by extrinsic cues: a Drosophila perspective

  2022cites this paper
• The Fly Blood-Brain Barrier Fights Against Nutritional Stress

  2022cites this paper
• Juvenile hormone receptor MET regulates sleep and neuronal morphology via glial-neuronal crosstalk.

  2021cites this paper
• Insulin signaling couples growth and early maturation to cholesterol intake

  2021cites this paper
• The Serine Protease Homolog, Scarface, Is Sensitive to Nutrient Availability and Modulates the Development of the Drosophila Blood–Brain Barrier

  2021cites this paper
• Growth regulation by amino acid transporters in Drosophila larvae

  2020cites this paper
• Interorgan communication in the control of metamorphosis.

  2020cites this paper
• The transcription factor CLAMP is required for neurogenesis in Drosophila melanogaster

  2020cites this paper
• Regulation of Body Size and Growth Control

  2020cites this paper
• Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation

  2020cites this paper
• Neuron–glia interaction in the Drosophila nervous system

  2020influential citation
• Metabolism and growth adaptation to environmental conditions in Drosophila

  2020cites this paper
• Growth and Maturation in Development: A Fly’s Perspective

  2020cites this paper
• Genetic variation in trophic avoidance shows fruit flies are 1 generally attracted to bacterial pathogens 2

  year unknowncites this paper